 
			 
			MCQOPTIONS
 Saved Bookmarks
				This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Biochemistry knowledge and support exam preparation. Choose a topic below to get started.
| 1. | Which of the following is Edman reagent? | 
| A. | Phenylisothiocyanate | 
| B. | CF3 COOH | 
| C. | FDNB | 
| D. | Phenylthiocarbonyl | 
| Answer» B. CF3 COOH | |
| 2. | What best summarizes the MALDI method by which gas phase ions are produced for mass spectrometry? | 
| A. | Sample is hit by a low energy xenon beam | 
| B. | Sample is forced through a narrow capillary tube and the solvent rapidly evaporates | 
| C. | Sample is embedded in a crystalline matrix and bombarded by laser beams | 
| D. | Sample is heated and then bombarded by electrons | 
| Answer» D. Sample is heated and then bombarded by electrons | |
| 3. | Edman degradation is used for _________ | 
| A. | Identifying N-terminal amino acids | 
| B. | Identifying C-terminal amino acids | 
| C. | Identifying amino acid | 
| D. | Identifying carbohydrates | 
| Answer» B. Identifying C-terminal amino acids | |
| 4. | Cleaving of peptide chain is done by _________ | 
| A. | Trypsin | 
| B. | Tyrosine | 
| C. | Tryptophan | 
| D. | Arginine | 
| Answer» B. Tyrosine | |
| 5. | The amino acid sequences of thousands of different proteins from many species have been determined using principles first developed by? | 
| A. | Edman | 
| B. | Sanger | 
| C. | Mendel | 
| D. | Watson and Crick | 
| Answer» C. Mendel | |
| 6. | Which of the following is a Sanger’s reagent? | 
| A. | 1-fluoro-2, 4-dinitrobenzene | 
| B. | 1-fluoro-2, 3-dinitrobenzene | 
| C. | 1-fluoro-2, 4-trinitrobenzene | 
| D. | 1-fluoro-2, 3-trinitrobenzene | 
| Answer» B. 1-fluoro-2, 3-dinitrobenzene | |
| 7. | Amino acids sequence in DNA can be determined by the order of their _________ | 
| A. | rRNA | 
| B. | tRNA | 
| C. | Nucleotides | 
| D. | mRNA | 
| Answer» D. mRNA | |
| 8. | Which_of_the_following_is_Edman_reagent?$ | 
| A. | Phenylisothiocyanate | 
| B. | CF<sub>3</sub> COOH | 
| C. | FDNB | 
| D. | Phenylthiocarbonyl | 
| Answer» B. CF<sub>3</sub> COOH | |
| 9. | Edman degradation is used fo? | 
| A. | Identifying N-terminal amino acids | 
| B. | Identifying C-terminal amino acids | 
| C. | Identifying amino acid | 
| D. | Identifying carbohydrates | 
| Answer» B. Identifying C-terminal amino acids | |
| 10. | Which of the following is the correct order of sequencing? | 
| A. | Cleaving, sequencing and ordering | 
| B. | Sequencing, ordering and cleaving | 
| C. | Ordering, cleaving and sequencing | 
| D. | Ordering, sequencing and cleaving | 
| Answer» B. Sequencing, ordering and cleaving | |
| 11. | Cleaving of peptide chain is done by | 
| A. | Trypsin | 
| B. | Tyrosine | 
| C. | Tryptophan | 
| D. | Arginine | 
| Answer» B. Tyrosine | |
| 12. | Which of the following compound is not involved in Edman degradation? | 
| A. | Phenylisothiocyanate | 
| B. | CF<sub>3</sub> COOH | 
| C. | FDNB | 
| D. | Phenylthiocarbonyl | 
| Answer» D. Phenylthiocarbonyl | |
| 13. | The amino acid sequences of thousands of different proteins from many species have been determined using principles first developed by | 
| A. | Edman | 
| B. | Sanger | 
| C. | Mendel | 
| D. | Watson and Crick | 
| Answer» C. Mendel | |
| 14. | Which of the following is a Sanger’s reagent?$ | 
| A. | 1-fluoro-2, 4-dinitrobenzene | 
| B. | 1-fluoro-2, 3-dinitrobenzene | 
| C. | 1-fluoro-2, 4-trinitrobenzene | 
| D. | 1-fluoro-2, 3-trinitrobenzene | 
| Answer» B. 1-fluoro-2, 3-dinitrobenzene | |
| 15. | Amino acids sequence in DNA can be determined by the order of their | 
| A. | rRNA | 
| B. | tRNA | 
| C. | Nucleotides | 
| D. | mRNA | 
| Answer» D. mRNA | |