Explore topic-wise MCQs in Protein Engineering.

This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Protein Engineering knowledge and support exam preparation. Choose a topic below to get started.

1.

Families of proteins with homologous tertiary structures that have diverged from a common ancestor show amino acid sequence differences.

A. False
B. True
Answer» C.
2.

Which of the following is not a step of the protein engineering cycle?

A. Gene cloning
B. Protein assay and characterization
C. 2D NMR
D. Mass spectrometry
Answer» E.
3.

Which of the following is not true for protein engineering?

A. Protein assay is a step in protein engineering
B. It requires a knowledge-based design
C. Preparative biochemistry is performed before protein assay
D. It does not require gene cloning
Answer» E.
4.

Which of the following technique is of no use in protein engineering?

A. X-ray crystallography
B. Gene cloning
C. Protein sequencing
D. Lyophilization
Answer» E.
5.

Which of the following step is performed before site-directed mutagenesis?

A. Expression
B. Protein assay
C. Preparative biochemistry
D. Knowledge-based design of novel protein
Answer» E.
6.

Which step is performed after the protein assay and characterization in the protein engineering cycle?

A. Gene cloning
B. Site-directed mutagenesis
C. Preparative biochemistry
D. Protein-ligand complex analysis
Answer» E.
7.

Which of the following is the process of developing proteins with the desired function by manipulating the stability and specificity of a protein?

A. Protein amplification
B. Protein assay
C. Protein expression
D. Protein engineering
Answer» E.
8.

Which of the following step is followed by the 2D NMR step in the protein engineering cycle?

A. Site-directed mutagenesis
B. Gene cloning
C. Protein assay
D. Display of 3D structure on computer graphics
Answer» E.
9.

Usually, only one cycle is sufficient to reach an optimally designed protein in sufficient quantities.

A. True
B. False
Answer» C.
10.

Which step is performed after the directed mutagenesis of the gene?

A. Gene recombination
B. 2D NMR
C. Gene editing
D. Expression
Answer» E.
11.

In the protein engineering cycle, the three-dimensional structure of the protein-ligand complex is defined by which of the following techniques?

A. HPLC
B. Mass spectrometry
C. Optical spectroscopy
D. High-resolution X-ray analysis
Answer» E.
12.

Which of the following step is performed just after the protein from a natural organism is isolated?

A. 2D NMR studies
B. Gene cloning
C. Site-directed mutagenesis
D. Preparative biochemistry
Answer» E.
13.

Random mutagenesis followed by selection at the level of the whole organism is a quick process.

A. True
B. False
Answer» C.
14.

Which of the following is not an application of protein engineering?

A. Synthesis of chimeric proteins
B. Modification of natural proteins
C. Construction of novel proteins
D. Multiplication of natural proteins
Answer» E.
15.

Which of the following technology offers new possibilities for modification of natural proteins for specific industrial, clinical, or agricultural purposes?

A. Protein structural studies
B. Protein sequencing
C. Protein assay
D. Protein engineering
Answer» E.