MCQOPTIONS
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This section includes 8 Mcqs, each offering curated multiple-choice questions to sharpen your Biochemistry knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Cleavage of DNA by RI endonuclease results in the formation of |
| A. | two 3'-OH ends and two 5'-phosphate ends |
| B. | one 3'-OH end and one 5'-phosphate end |
| C. | two 3'-phosphate ends and two 3'-OH ends |
| D. | one 3'-OH end and two 5'-phosphate end |
| Answer» B. one 3'-OH end and one 5'-phosphate end | |
| 2. |
Cleavage of DNA by EcoRI endonuclease results in the formation of |
| A. | two 3'-OH ends and two 5'-phosphate ends |
| B. | one 3'-OH end and one 5'-phosphate end |
| C. | two 3'-phosphate ends and two 3'-OH ends |
| D. | one 3'-OH end and two 5'-phosphate end |
| Answer» B. one 3'-OH end and one 5'-phosphate end | |
| 3. |
Which of the following amino acids can interact with the DNA or RNA nucleotide bases via hydrogen bonding? |
| A. | Asn and Gln |
| B. | Cys and Met |
| C. | Lys and Leu |
| D. | Ile and Val |
| Answer» B. Cys and Met | |
| 4. |
The type II restriction endonucleases, bind to DNA sites |
| A. | using H-bonds for specificity |
| B. | that are usually palindromic |
| C. | using non-sequence-dependent backbone interactions |
| D. | all of the above |
| Answer» E. | |
| 5. |
The enzyme that joins DNA cuts is called |
| A. | joinase |
| B. | ligase |
| C. | DNA phosphorylase |
| D. | reverse transcriptase |
| Answer» C. DNA phosphorylase | |
| 6. |
Reverse transcripase from HIV does not |
| A. | uses RNA as a template |
| B. | has a 5' to 3' polymerase activity |
| C. | has a 3'- 5' exonuclease activity |
| D. | requires a primer |
| Answer» D. requires a primer | |
| 7. |
Different DNA polymerases play distinct roles in DNA replication and repair in both prokaryotic and eukaryotic cells. All known DNA polymerases synthesize DNA only in the __________ by the addition of dNTPs to a performed primer strand of DNA. |
| A. | positive direction |
| B. | 3' to 5' direction |
| C. | 5' to 3' direction |
| D. | negative direction |
| Answer» D. negative direction | |
| 8. |
Which of the following amino acids interact with the DNA or RNA backbone (i.e. phosphate)? |
| A. | Ile and Val |
| B. | Lys and Arg |
| C. | Leu and Ala |
| D. | Cys and Met |
| Answer» C. Leu and Ala | |