MCQOPTIONS
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MCQOPTIONS
This section includes 199 Mcqs, each offering curated multiple-choice questions to sharpen your Biochemistry knowledge and support exam preparation. Choose a topic below to get started.
| 151. |
Restriction maps |
| A. | allows comparison between DNA molecules without the need to determine nucleotide sequence |
| B. | allows comparison between DNA molecules but requires to determine nucleotide sequence |
| C. | does not allow comparison between DNA molecules |
| D. | none of the above |
| Answer» B. allows comparison between DNA molecules but requires to determine nucleotide sequence | |
| 152. |
ATP is from which general category of molecules? |
| A. | Polysaccharides |
| B. | Proteins |
| C. | Nucleotides |
| D. | Amino acids |
| Answer» D. Amino acids | |
| 153. |
In glycolysis, ATP is formed by the transfer of a high-energy phosphate from 1,3-bisphosphoglycerate to ADR No such high-energy phosphate donor has ever been isolated in mitochondria because |
| A. | the techniques for isolating the phosphate donor are not refined enough |
| B. | no such phosphate donor exists |
| C. | the high-energy phosphate donor is very short-lived and difficult to isolate |
| D. | None of the above |
| Answer» C. the high-energy phosphate donor is very short-lived and difficult to isolate | |
| 154. |
Which of the following is not true of glycolysis? |
| A. | ADP is phosphorylated to ATP via substrate level phosphorylation |
| B. | The pathway does not require oxygen |
| C. | The pathway oxidizes two moles of NADH to NAD+ for each mole of glucose that enters |
| D. | The pathway requires two moles of ATP to get started catabo-lizing each mole of glucose |
| Answer» D. The pathway requires two moles of ATP to get started catabo-lizing each mole of glucose | |
| 155. |
When concentration of the reactants is higher than the equilibrium concentration then |
| A. | the gibbs free energy will be positive |
| B. | the gibbs free energy will be negative |
| C. | more products will be formed |
| D. | both (b) and (c) |
| Answer» E. | |
| 156. |
The glycolytic pathway (glucose → 2 pyruvate) is found |
| A. | in all living organisms |
| B. | primarily in animals excluding particles |
| C. | only in eukaryotes |
| D. | only in yeast |
| Answer» B. primarily in animals excluding particles | |
| 157. |
DNA fragments in a restriction digest can be separated by electrophoresis in |
| A. | poly acrylamide |
| B. | agarose gel |
| C. | both (a) and (b) |
| D. | none of these |
| Answer» D. none of these | |
| 158. |
What technique can be used to determine the murderer who left blood with the victim? |
| A. | DNA sequencing |
| B. | PCR amplification |
| C. | Western blot |
| D. | RFLP mapping |
| Answer» C. Western blot | |
| 159. |
Double stranded DNA denaturation with specified limit of temperature is |
| A. | reversible reaction |
| B. | irreversible reaction |
| C. | either (a) or (b) |
| D. | none of these |
| Answer» B. irreversible reaction | |
| 160. |
The viruses that can achieve neoplastic transformation are called |
| A. | DNA tumor viruses |
| B. | RNA tumor viruses |
| C. | retroviruses HIV |
| D. | none of these |
| Answer» B. RNA tumor viruses | |
| 161. |
For gene transfer to be effective, transforming DNA must be |
| A. | incorporated into the bacterial chromosome |
| B. | incorporated into a viral genome |
| C. | free in the bacterial cytoplasm |
| D. | none of the above |
| Answer» B. incorporated into a viral genome | |
| 162. |
Which of the following regulates glycolysis steps? |
| A. | Phosphofructokinase |
| B. | Hexose kinase |
| C. | Pyruvate kinase |
| D. | All of these |
| Answer» E. | |
| 163. |
Fructose-2,6-bisphosphate |
| A. | activates fructose-1,6-bisphosphatase |
| B. | activates phosphofructokinase |
| C. | inhibits fructose-1,6-bisphosphatase |
| D. | both (b) and (c) |
| Answer» E. | |
| 164. |
Glycogen has |
| A. | α-1,4 linkage |
| B. | α-1,6 linkages |
| C. | α-1,4 and α-1,6 linkages |
| D. | α-1,4 and β-1,6 linkage |
| Answer» D. α-1,4 and β-1,6 linkage | |
| 165. |
Glucose from the breakdown of glycogen is obtained in |
| A. | the liver by phosphorolysis |
| B. | the muscles by phosphorolysis |
| C. | the muscles by hydrolysis |
| D. | both (a) and (b) |
| Answer» E. | |
| 166. |
What condition is caused by iodine deficiency during pregnancy and is characterized by stunted growth, deafness, and mental retardation? |
| A. | Cretinism |
| B. | Keshan disease |
| C. | Multiple sclerosis |
| D. | Crohn's disease |
| Answer» B. Keshan disease | |
| 167. |
Chronic consumption of excess selenium can lead to |
| A. | brittle hair and nails. |
| B. | muscle weakness, |
| C. | TPN |
| D. | diarrhea. |
| Answer» C. TPN | |
| 168. |
During glycolysis, the major energy generating step involves |
| A. | pyruvate kinase |
| B. | phosphoglycerate kinase |
| C. | glyceraldehyde-3 -dehydrogenase |
| D. | Phosphofructokinase |
| Answer» D. Phosphofructokinase | |
| 169. |
The general term used for the anaerobic degradation of glucose to obtain energy is |
| A. | Anabolism |
| B. | Oxidation |
| C. | Fermentation |
| D. | Metabolism |
| Answer» D. Metabolism | |
| 170. |
Unfolding of regular secondary protein structure causes |
| A. | Large decrease in the entropy of the protein |
| B. | Little increase in the entropy of protein |
| C. | No change in the entropy of the protein |
| D. | Large increase in the entropy of the protein |
| Answer» E. | |
| 171. |
The study of energy relationships and conversions in biological systems is called as |
| A. | Biophysics |
| B. | Biotechnology |
| C. | Bioenergetics |
| D. | Microbiology |
| Answer» D. Microbiology | |
| 172. |
The PCR, polymerase chain reaction is becoming the method of choice for |
| A. | Alteration of gene |
| B. | screening gene |
| C. | sterilization of gene |
| D. | all of these |
| Answer» C. sterilization of gene | |
| 173. |
Site directed mutagenesis facilitated research on |
| A. | Carbohydrates |
| B. | Proteins |
| C. | Lipids |
| D. | Fats |
| Answer» C. Lipids | |
| 174. |
Dextrin hydrolysis yields |
| A. | D-galactose and D-glucose |
| B. | 2-Dglucose |
| C. | n-Dglucose |
| D. | 2-Dfructose |
| Answer» D. 2-Dfructose | |
| 175. |
Glycolysis converts |
| A. | Glucose into pyruvate |
| B. | Glucose into phosphoenolpyruvate |
| C. | Fructose into pyruvate |
| D. | Fructose into phosphoenolpyruvate |
| Answer» B. Glucose into phosphoenolpyruvate | |
| 176. |
The product formed in the first substrate level phosphorylation in glycolysis is |
| A. | Pyruvate |
| B. | 3-phosphoglycerate |
| C. | 1, 3-bisphosphoglycerate |
| D. | 2-phosphoglycerate |
| Answer» C. 1, 3-bisphosphoglycerate | |
| 177. |
An endemic disease of cardiomyopathy in children is attributed to |
| A. | low Se in the environment |
| B. | low intake of iron |
| C. | high Se in the environment |
| D. | high intake of iron |
| Answer» B. low intake of iron | |
| 178. |
Manganese is a key component of all of the following enzymes except |
| A. | arginase |
| B. | pyruvate carboxylase |
| C. | ceruloplasmin |
| D. | Mn-superoxide dismutase |
| Answer» D. Mn-superoxide dismutase | |
| 179. |
In a PCR reaction after four cycles, each molecule of a duplex DNA will give rise to |
| A. | 16 single strands of DNA |
| B. | 16 double stranded DNA |
| C. | 18 single strands of DNA |
| D. | 18 double stranded DNA |
| Answer» B. 16 double stranded DNA | |
| 180. |
Consumption of which nutrient leads to the multiplication of oral bacteria? |
| A. | Fat |
| B. | Carbohydrate |
| C. | Protein |
| D. | Fluoride |
| Answer» C. Protein | |
| 181. |
The mineral which is considered important in maintaining electrical potential in nerves and membranes is |
| A. | magnesium |
| B. | manganese |
| C. | calcium |
| D. | iron |
| Answer» B. manganese | |
| 182. |
TPP (thiamine pyrophosphate) is derived from |
| A. | Vitamin A |
| B. | Vitamin B1 |
| C. | Vitamin C |
| D. | Vitamin B2 |
| Answer» C. Vitamin C | |
| 183. |
In the reduction of pyruvate to lactate, which of the following is regenerated? |
| A. | H⁺ |
| B. | NADH |
| C. | NAD⁺ |
| D. | Na⁺ |
| Answer» D. Na⁺ | |
| 184. |
For its activity, pyruvate decarboxylase requires |
| A. | Mg⁺² |
| B. | Ca⁺² |
| C. | Na⁺ |
| D. | H⁺ |
| Answer» B. Ca⁺² | |
| 185. |
For every one molecule of sugar glucose which is oxidized __________ molecule of pyruvic acid are produced. |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» C. 3 | |
| 186. |
The enzymes of glycolysis in a eukaryotic cell are located in the |
| A. | intermembrane space |
| B. | plasma membrane |
| C. | cytosol |
| D. | mitochondrial matrix |
| Answer» D. mitochondrial matrix | |
| 187. |
The amount of energy received from one ATP is |
| A. | 76 kcal |
| B. | 7.3 kcal |
| C. | 760 kcal |
| D. | 1000 kcal |
| Answer» C. 760 kcal | |
| 188. |
The active form of glycogen phosphorylase is phosphorylated, while the dephosphorylation of which active form occurs? |
| A. | Glycogen synthase |
| B. | Glycogen semisynthase |
| C. | Glycogen hydrolase |
| D. | Glycogen dehydrogenase |
| Answer» B. Glycogen semisynthase | |
| 189. |
In the glycogen synthase reaction, the precursor to glycogen is |
| A. | glucose-6-P |
| B. | UTP-glucose |
| C. | UDP-glucose |
| D. | glucose-1-P |
| Answer» D. glucose-1-P | |
| 190. |
Phosphofructokinase, the major flux-controlling enzyme of glycolysis is allosterically inhibited and activated respectively by |
| A. | ATP and PEP |
| B. | AMP and Pi |
| C. | ATP and ADP |
| D. | Citrate and ATP |
| Answer» D. Citrate and ATP | |
| 191. |
From a single molecule of DNA, PCR can make |
| A. | one additional copy |
| B. | hundreds of copies |
| C. | thousands of copies |
| D. | millions of copies |
| Answer» E. | |
| 192. |
Which of the following is not a mechanism for altering the flux of metabolites through the rate-determining step of a pathway? |
| A. | Allosteric control of the enzyme activity |
| B. | Diffusional coupling between adjacent active sites |
| C. | Genetic control of the enzyme concentration |
| D. | Covalent modification of the enzyme |
| Answer» C. Genetic control of the enzyme concentration | |
| 193. |
Genomic libraries are made from |
| A. | genomic DNA of an organism |
| B. | genomic RNA of an organism |
| C. | genomic cDNA of an organism |
| D. | genomic mRNA of an organism |
| Answer» B. genomic RNA of an organism | |
| 194. |
Which of the following could act as an uncoupler of electron transport and ATP synthesis? |
| A. | The Fo base-piece of ATP synthase (without the Fl subunit) |
| B. | Dinitrophenol |
| C. | neither (a) nor (b) |
| D. | Both (a) and (b) |
| Answer» E. | |
| 195. |
To clone into a plasmid vector, both the plasmid and the foreign DNA are cut |
| A. | with the same restriction enzyme and mixed together |
| B. | with different restriction enzyme and mixed together |
| C. | with the combination of enzymes and then seperated |
| D. | with the combination of enzymes and mixed together |
| Answer» B. with different restriction enzyme and mixed together | |
| 196. |
PCR is used |
| A. | to diagnose genetic diseases |
| B. | to solve crimes |
| C. | to study gene function |
| D. | all of these |
| Answer» E. | |
| 197. |
Bacteriophages are |
| A. | cells in the blood that eat bacteria |
| B. | a class of bacteria |
| C. | bacterial viruses |
| D. | none of the above |
| Answer» D. none of the above | |
| 198. |
Specialized transduction occurs when |
| A. | the bacteriophage incorporates randomly in the bacterial chromosome |
| B. | the bacteriophage never incorporates into the bacterial chromosome |
| C. | the bacteriophage always incorporates at the same position in the bacterial chromosome |
| D. | none of the above |
| Answer» D. none of the above | |
| 199. |
PCR can be used to amplify a specific fragment of DNA from which of the following? |
| A. | A drop of blood |
| B. | A hair follicle |
| C. | A fragment of skin |
| D. | All of these |
| Answer» E. | |