MCQOPTIONS
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MCQOPTIONS
This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Protein Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
A polypeptide chain contains two terminals – one carboxyl-terminal (C terminal) and the other amino-terminal (N terminal). Which of the following is true? |
| A. | N terminal is synthesized last during translation |
| B. | C terminal is synthesized first during translation |
| C. | N terminal is represented on the right side and C terminal on the left side |
| D. | N terminal is represented on the left side and C terminal on the right side |
| Answer» E. | |
| 2. |
A protein molecule contains amino acid residues and not amino acids because when a peptide bond is formed _________ is lost. |
| A. | nitrogen molecule |
| B. | hydrogen molecule |
| C. | oxygen molecule |
| D. | water molecule |
| Answer» E. | |
| 3. |
The primary structure of a protein is held together by non-covalent interactions such as peptide bonds, made during transcription. |
| A. | True |
| B. | False |
| Answer» C. | |
| 4. |
Which of the following is related to the primary structure of proteins? |
| A. | Alpha helix |
| B. | Beta-sheets |
| C. | Loops |
| D. | Amino acid sequence |
| Answer» E. | |
| 5. |
______________ is used for sequencing of peptides. |
| A. | X-ray crystallography |
| B. | Spectrophotometry |
| C. | NMR spectroscopy |
| D. | Mass spectrometry |
| Answer» E. | |
| 6. |
Cyanogen bromide (CNBr) cleaves the peptide bond on the ‘C’ terminal side of the _______ residues. |
| A. | Trp |
| B. | Ser |
| C. | Ala |
| D. | Met |
| Answer» E. | |
| 7. |
Trypsin cleaves peptide bonds on the ‘c’ terminal side of the positively charged residues Arg and Lys, if the next residues is not _____ |
| A. | His |
| B. | Gly |
| C. | Ala |
| D. | Pro |
| Answer» E. | |
| 8. |
__________ is used as a reducing agent in SDS PAGE. |
| A. | Sodium dodecyl sulfate (SDS) |
| B. | Ammonium persulphate (APS) |
| C. | Bisacrylamide |
| D. | 2-mercaptoethanol |
| Answer» E. | |
| 9. |
The polyacrylamide gel electrophoresis (PAGE) of proteins is based on the following properties of proteins. |
| A. | Size and charge only |
| B. | Size and shape only |
| C. | Electric charge only |
| D. | Size, shape and electric charge |
| Answer» E. | |
| 10. |
In metal chelate affinity chromatography, divalent cations such as Zn+2 or Ni+2 is attached on the electrophoretic matrix and it binds to ___________ |
| A. | Trp tag |
| B. | Ala tag |
| C. | Gly tag |
| D. | His tag |
| Answer» E. | |
| 11. |
The minimum number of residues that appear to be necessary for a polypeptide to fold into a discrete and stable shape that allows it to carry out a function is near 40. |
| A. | False |
| B. | True |
| Answer» C. | |
| 12. |
For a protein with 100 amino acids, how many possible sequences are there? |
| A. | (100)20 |
| B. | (2)100 |
| C. | (100)2 |
| D. | (20)100 |
| Answer» E. | |
| 13. |
Which of the following represents the two-dimensional structure of proteins? |
| A. | Quaternary |
| B. | Tertiary |
| C. | Secondary |
| D. | Primary |
| Answer» E. | |
| 14. |
Which of the following interactions is crucial for the primary structure of proteins? |
| A. | Hydrogen bond |
| B. | Di-sulfide bond |
| C. | Vander Waals interactions |
| D. | Peptide bond |
| Answer» E. | |
| 15. |
The structure formed by joining the amino acids by a peptide bond is called ________ structure of a protein. |
| A. | quaternary |
| B. | tertiary |
| C. | secondary |
| D. | primary |
| Answer» E. | |