MCQOPTIONS
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MCQOPTIONS
This section includes 26 Mcqs, each offering curated multiple-choice questions to sharpen your Biochemistry knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Transformation means |
| A. | formation of a pilus |
| B. | acquiring DNA from the bacterial cell environment |
| C. | plasmid containing a F factor |
| D. | F+ and F- strains of bacteria |
| Answer» C. plasmid containing a F factor | |
| 2. |
A PCR cycle consists of |
| A. | three steps, denaturation, primer annealing and elongation |
| B. | three steps, denaturation, initiation and elongation |
| C. | three steps, primer annealing, elongation and termination |
| D. | three steps, initiation, elongation and termination |
| Answer» B. three steps, denaturation, initiation and elongation | |
| 3. |
Bacteriophages are |
| A. | cells in the blood that eat bacteria |
| B. | a class of bacteria |
| C. | bacterial viruses |
| D. | none of the above |
| Answer» D. none of the above | |
| 4. |
PCR can be used in |
| A. | cloning |
| B. | sequencing |
| C. | medical diagnosis and forensic medicine |
| D. | all of the above |
| Answer» E. | |
| 5. |
PCR (Polymerase Chain Reaction) can make multiple copies of |
| A. | DNA |
| B. | RNA |
| C. | protein |
| D. | carbohydrate |
| Answer» B. RNA | |
| 6. |
PCR is an abbreviation for |
| A. | Protein Change Reaction |
| B. | Polymerase Chain Reaction |
| C. | Positive Charge Reaction: |
| D. | Preliminary Change Rate |
| Answer» C. Positive Charge Reaction: | |
| 7. |
Southern blotting involves |
| A. | electrophoresis of DNA molecules and then blotting the separated DNA bands followed by incubation |
| B. | electrophoresis of DNA molecules and then blotting the separated RNA bands followed by incubation |
| C. | electrophoresis of DNA molecules followed by incubation |
| D. | electrophoresis of RNA molecules and then blotting the separated RNA bands followed by incubation |
| Answer» B. electrophoresis of DNA molecules and then blotting the separated RNA bands followed by incubation | |
| 8. |
PCR is used |
| A. | to diagnose genetic diseases |
| B. | to solve crimes |
| C. | to study gene function |
| D. | all of these |
| Answer» E. | |
| 9. |
To clone into a plasmid vector, both the plasmid and the foreign DNA are cut |
| A. | with the same restriction enzyme and mixed together |
| B. | with different restriction enzyme and mixed together |
| C. | with the combination of enzymes and then seperated |
| D. | with the combination of enzymes and mixed together |
| Answer» B. with different restriction enzyme and mixed together | |
| 10. |
PCR can be used to amplify a specific fragment of DNA from which of the following? |
| A. | A drop of blood |
| B. | A hair follicle |
| C. | A fragment of skin |
| D. | All of these |
| Answer» E. | |
| 11. |
Bactriophages absorb to a bacterial surface and inject the phage DNA through the |
| A. | cell wall into cytosol |
| B. | cell wall into plasmamembrane |
| C. | both (a) and (b) |
| D. | cell wall into endoplasmic reticulum |
| Answer» B. cell wall into plasmamembrane | |
| 12. |
Additional mono saccharides are added in Golgi to produce |
| A. | either high mannose type oligosaccharide or a complex type |
| B. | either fructose type monosaccharide or a complex type |
| C. | either high sucrose type polysaccharide or a complex type |
| D. | none of the above |
| Answer» B. either fructose type monosaccharide or a complex type | |
| 13. |
The PCR, polymerase chain reaction is becoming the method of choice for |
| A. | Alteration of gene |
| B. | screening gene |
| C. | sterilization of gene |
| D. | all of these |
| Answer» C. sterilization of gene | |
| 14. |
Specialized transduction occurs when |
| A. | the bacteriophage incorporates randomly in the bacterial chromosome |
| B. | the bacteriophage never incorporates into the bacterial chromosome |
| C. | the bacteriophage always incorporates at the same position in the bacterial chromosome |
| D. | none of the above |
| Answer» D. none of the above | |
| 15. |
DNA fragments in a restriction digest can be separated by electrophoresis in |
| A. | poly acrylamide |
| B. | agarose gel |
| C. | both (a) and (b) |
| D. | none of these |
| Answer» D. none of these | |
| 16. |
Double stranded DNA denaturation with specified limit of temperature is |
| A. | reversible reaction |
| B. | irreversible reaction |
| C. | either (a) or (b) |
| D. | none of these |
| Answer» B. irreversible reaction | |
| 17. |
Genomic libraries are made from |
| A. | genomic DNA of an organism |
| B. | genomic RNA of an organism |
| C. | genomic cDNA of an organism |
| D. | genomic mRNA of an organism |
| Answer» B. genomic RNA of an organism | |
| 18. |
The viruses that can achieve neoplastic transformation are called |
| A. | DNA tumor viruses |
| B. | RNA tumor viruses |
| C. | retroviruses HIV |
| D. | none of these |
| Answer» B. RNA tumor viruses | |
| 19. |
From a single molecule of DNA, PCR can make |
| A. | one additional copy |
| B. | hundreds of copies |
| C. | thousands of copies |
| D. | millions of copies |
| Answer» E. | |
| 20. |
For gene transfer to be effective, transforming DNA must be |
| A. | incorporated into the bacterial chromosome |
| B. | incorporated into a viral genome |
| C. | free in the bacterial cytoplasm |
| D. | none of the above |
| Answer» B. incorporated into a viral genome | |
| 21. |
PCR amplifies millions of copies of |
| A. | a short fragment of DNA |
| B. | the entire genome (all the DNA in a sample) |
| C. | both (a) and (b) |
| D. | short chain RNA |
| Answer» B. the entire genome (all the DNA in a sample) | |
| 22. |
Restriction maps |
| A. | allows comparison between DNA molecules without the need to determine nucleotide sequence |
| B. | allows comparison between DNA molecules but requires to determine nucleotide sequence |
| C. | does not allow comparison between DNA molecules |
| D. | none of the above |
| Answer» B. allows comparison between DNA molecules but requires to determine nucleotide sequence | |
| 23. |
How many ds DNA molecule can be produced, which comprise precisely the target region in double strand form during 4th cycle of PCR? |
| A. | Two ds DNA molecule |
| B. | Three ds DNA molecule |
| C. | Eight ds DNA molecule |
| D. | Four ds DNA molecule |
| Answer» E. | |
| 24. |
In a PCR reaction after four cycles, each molecule of a duplex DNA will give rise to |
| A. | 16 single strands of DNA |
| B. | 16 double stranded DNA |
| C. | 18 single strands of DNA |
| D. | 18 double stranded DNA |
| Answer» B. 16 double stranded DNA | |
| 25. |
Northern blotting is |
| A. | widely different than southern blotting |
| B. | another name for southern blotting |
| C. | analogous to southern blotting |
| D. | none of the above |
| Answer» D. none of the above | |
| 26. |
What technique can be used to determine the murderer who left blood with the victim? |
| A. | DNA sequencing |
| B. | PCR amplification |
| C. | Western blot |
| D. | RFLP mapping |
| Answer» C. Western blot | |