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				This section includes 50 Mcqs, each offering curated multiple-choice questions to sharpen your Microbiology knowledge and support exam preparation. Choose a topic below to get started.
| 1. | If a 1:600 dilution of a test compound kills a standard population of in 10 minutes but not 5 minutes while a 1:60 dilution of phenol kills the population in the same time, what is the phenol coefficient of the test compound? | 
| A. | 1 | 
| B. | 5 | 
| C. | 10 | 
| D. | 50 | 
| Answer» D. 50 | |
| 2. | Which disinfectant was the most effective against "? | 
| A. | Phenol | 
| B. | Cetylpyridinium chloride | 
| C. | Hexachlorophene | 
| D. | Merthiolate | 
| Answer» E. | |
| 3. | Which disinfectant would be most effective against found in a blood spill? | 
| A. | Phenol | 
| B. | Cetylpyridinium chloride | 
| C. | Hexachlorophene | 
| D. | None of these | 
| Answer» E. | |
| 4. | The time required to kill 90% of the microorganisms in a sample at a specific temperature is the | 
| A. | decimal reduction time | 
| B. | thermal death point | 
| C. | F value | 
| D. | D value | 
| Answer» E. | |
| 5. | Which of the following articles can be sterilized in an autoclave? | 
| A. | Gloves | 
| B. | Culture media | 
| C. | Dressing material | 
| D. | All of these | 
| Answer» E. | |
| 6. | Which of the following disinfectants act by disrupting microbial membranes? | 
| A. | Cationic detergents | 
| B. | Halogens | 
| C. | Heavy metals | 
| D. | Aldehydes | 
| Answer» B. Halogens | |
| 7. | Which of the following is best to sterilize heat labile solutions? | 
| A. | Dry heat | 
| B. | Autoclave | 
| C. | Membrane filtration | 
| D. | Pasteurization | 
| Answer» D. Pasteurization | |
| 8. | Which of the following is best used for long term storage of microbial samples when carried out properly? | 
| A. | Storage in a freezer at -10 C | 
| B. | Storage in a freezer at ultra low temperatures (-70 C) | 
| C. | Storage in a refrigerator on an agar slant | 
| D. | Storage on a petri plate at room temperature | 
| Answer» C. Storage in a refrigerator on an agar slant | |
| 9. | Which of the following compounds are commonly used as general antiseptics to treat cuts and scratches? | 
| A. | Aldehydes | 
| B. | Ethylene oxide | 
| C. | Halogens | 
| D. | None of these | 
| Answer» D. None of these | |
| 10. | Which of the following is a limitation of the autoclave? | 
| A. | It takes too long to sterilize | 
| B. | It lacks the ability to inactivate viruses | 
| C. | It lacks the ability to kill endospores | 
| D. | It will destroy heat labile materials | 
| Answer» E. | |
| 11. | Which of the following has most heat resistant spores? | 
| A. | <i>Clostridium histolyticum</i> | 
| B. | <i>Clostridium perfringens </i> | 
| C. | <i>Bacillus stearothermophilus</i> | 
| D. | <i> Bacillus cereus</i> | 
| Answer» D. <i> Bacillus cereus</i> | |
| 12. | The lowest temperature that kills all microorganisms in a liquid suspension in 10 minutes is known as the | 
| A. | decimal reduction time | 
| B. | thermal death point | 
| C. | thermal death temperature | 
| D. | thermal death time | 
| Answer» C. thermal death temperature | |
| 13. | Which of the following tests is designed to simulate the natural conditions under which the disinfectants are used in hospitals? | 
| A. | Phenol coefficient test | 
| B. | Minimum inhibitory concentration test | 
| C. | Capacity test | 
| D. | None of these | 
| Answer» D. None of these | |
| 14. | Which of the following was the first widely used antiseptic and disinfectant? | 
| A. | Chlorine | 
| B. | Phenol | 
| C. | Iodine | 
| D. | Alcohol | 
| Answer» C. Iodine | |
| 15. | Steam exposure of a material at 100 C for 20 minutes for three consecutive days is known as | 
| A. | inspissation | 
| B. | tyndallization | 
| C. | autoclaving | 
| D. | none of these | 
| Answer» C. autoclaving | |
| 16. | The organisms retained in the fluids filtered by Seitz filter is | 
| A. | <i>Proteus</i> | 
| B. | <i>Staphylococcus</i> | 
| C. | <i>Clostridium</i> | 
| D. | None of these | 
| Answer» E. | |
| 17. | For sterilization of which material gamma rays can be used? | 
| A. | Catheters | 
| B. | Plastic syringes | 
| C. | Canulas | 
| D. | None of these | 
| Answer» E. | |
| 18. | The time in minutes at a specific temperature needed to kill a population of cells is the | 
| A. | decimal reduction time | 
| B. | thermal death point | 
| C. | thermal death temperature | 
| D. | F value | 
| Answer» E. | |
| 19. | Which of the following is sterilized with the help of glutaraldehyde? | 
| A. | Thermometers | 
| B. | Bronchoscopes | 
| C. | Cystoscopes | 
| D. | All of these | 
| Answer» E. | |
| 20. | Microbes can be removed from a liquid solution by the process of | 
| A. | filtration | 
| B. | freeze-drying | 
| C. | osmosis | 
| D. | desiccation | 
| Answer» B. freeze-drying | |
| 21. | Which of the following process can be efficiently carried out by incineration? | 
| A. | Sterilization of scalpel blades and needles | 
| B. | Sterilization of all glass syringes | 
| C. | Sterilization of points of forceps | 
| D. | Destruction of contaminated materials | 
| Answer» E. | |
| 22. | The process of making an object free from living organisms including bacterial and fungal spores and viruses is known as | 
| A. | pasteurization | 
| B. | antisepsis | 
| C. | disinfection | 
| D. | sterilization | 
| Answer» E. | |
| 23. | Media containing spores and thermolabile constituents are sterilized by | 
| A. | pasteurization | 
| B. | UV radiation | 
| C. | dry heat | 
| D. | tyndalization | 
| Answer» C. dry heat | |
| 24. | A(n)____is used to prevent infection by killing or inhibiting pathogen growth on animal tissues. | 
| A. | bacteriostatic agent | 
| B. | sanitizer | 
| C. | disinfectant | 
| D. | antiseptic | 
| Answer» E. | |
| 25. | One drawback to the use of ultraviolet light as a sterilizing agent is its | 
| A. | failure to kill bacteria | 
| B. | failure to bacterial spores | 
| C. | failure to kill microbes in a closed environment | 
| D. | failure to kill microbes located in the center of an object | 
| Answer» E. | |
| 26. | Ethylene oxide is used to destroy or kill which of the following microbes? | 
| A. | Bacteria | 
| B. | Spores | 
| C. | Fungi | 
| D. | All of these | 
| Answer» E. | |
| 27. | Bactericidal concentration of phenol is | 
| A. | 0.25% | 
| B. | 0.5% | 
| C. | 0.75% | 
| D. | 1% | 
| Answer» E. | |
| 28. | Which of the following skin disinfectant(s) is/are most frequently used? | 
| A. | Isopropyl alcohol | 
| B. | Ethyl alcohol | 
| C. | Both (a) and (b) | 
| D. | none of these | 
| Answer» D. none of these | |
| 29. | Preservation of foods by using salts and sugars works by | 
| A. | raising pH | 
| B. | lowering osmotic pressure | 
| C. | creating a hypertonic environment | 
| D. | creating a hypotonic environment | 
| Answer» D. creating a hypotonic environment | |
| 30. | Which of the following items could be sterilized by dry heat sterilization? | 
| A. | Intravenous (IV) solution | 
| B. | Plastic IV bags | 
| C. | Glass pipettes | 
| D. | rubber gloves | 
| Answer» D. rubber gloves | |
| 31. | Which of the following does not kill endospores? | 
| A. | Autoclave | 
| B. | Incineration | 
| C. | Hot air sterilization | 
| D. | Pasteurization | 
| Answer» E. | |
| 32. | Which of the following describes the effect of ionizing radiation on microbes? | 
| A. | Changes the 3-dimensional structure of proteins to a 2-dimen-sional form | 
| B. | Substantially decreases enzymatic activity, slowing microbial reproduction | 
| C. | Induces electrons and protons to jump out of the molecules of the microbe | 
| D. | None of the above | 
| Answer» D. None of the above | |
| 33. | Which is the most important surface active disinfectants? | 
| A. | Amphoteric compounds | 
| B. | Cationic compounds | 
| C. | Non-ionic compounds | 
| D. | Anionic compounds | 
| Answer» C. Non-ionic compounds | |
| 34. | Milk is pasteurized in batch method by keeping it at | 
| A. | 63 C for 30 minutes | 
| B. | 72 C for 60 seconds | 
| C. | 73 C for 30 minutes | 
| D. | 72 C for 6 minutes | 
| Answer» B. 72 C for 60 seconds | |
| 35. | Which disinfectant was the most effective against Salmonella"? | 
| A. | Phenol | 
| B. | Cetylpyridinium chloride | 
| C. | Hexachlorophene | 
| D. | Merthiolate | 
| Answer» E. | |
| 36. | If a canning procedure is not properly followed, which type of microbe is most likely to grow in the canned food? | 
| A. | Obligate Aerobe | 
| B. | Acidophile | 
| C. | Mesophile | 
| D. | Obligate Anaerobe | 
| Answer» E. | |
| 37. | Which of the following is not a disinfectant containing a heavy metal? | 
| A. | Silver nitrate | 
| B. | Mercurochrome | 
| C. | Copper sulfate | 
| D. | Chlorine | 
| Answer» E. | |
| 38. | Which disinfectant would be most effective against Staphylococcus found in a blood spill? | 
| A. | Phenol | 
| B. | Cetylpyridinium chloride | 
| C. | Hexachlorophene | 
| D. | None of these | 
| Answer» E. | |
| 39. | Complete destruction of the microorganisms is commonly known as | 
| A. | disinfection | 
| B. | sterilization | 
| C. | antisepsis | 
| D. | none of these | 
| Answer» D. none of these | |
| 40. | Which of the following material is sterilized by use of red heat? | 
| A. | Soiled dressings | 
| B. | Inoculating wires and loops | 
| C. | Glass slides | 
| D. | All-glass syringes | 
| Answer» C. Glass slides | |
| 41. | Which of the following methods will not sterilize? | 
| A. | Aqueous glutaraldehyde for 10 hours | 
| B. | Dry heat at 171 C for 1 to 2 hours | 
| C. | 121 C at 15 lb/in pressure for 15 to 20 minutes | 
| D. | 100 C boiling water for 30 minutes | 
| Answer» E. | |
| 42. | Which of the following is/are vapour-phase disinfectant/s? | 
| A. | Formaldehyde | 
| B. | Ethylene oxide | 
| C. | Both (a) and (b) | 
| D. | None of these | 
| Answer» D. None of these | |
| 43. | Sweet and salty foods frequently don't require refrigeration to prevent spoilage because they have | 
| A. | insufficient nutrients | 
| B. | low pH | 
| C. | high concentration of solutes | 
| D. | toxic alkaline chemicals | 
| Answer» D. toxic alkaline chemicals | |
| 44. | Iodophores are mixture of | 
| A. | iodine and aldehydes | 
| B. | iodine and surface active agents | 
| C. | iodine and alcohols | 
| D. | iodine and phenols | 
| Answer» C. iodine and alcohols | |
| 45. | If a 1:600 dilution of a test compound kills a standard population of Staphylococcus aureus in 10 minutes but not 5 minutes while a 1:60 dilution of phenol kills the population in the same time, what is the phenol coefficient of the test compound? | 
| A. | 1 | 
| B. | 5 | 
| C. | 10 | 
| D. | 50 | 
| Answer» D. 50 | |
| 46. | Which of the following substances can sterilize? | 
| A. | Alcohol | 
| B. | Cetylpyridinium chloride | 
| C. | Ethylene oxide | 
| D. | Chlorine | 
| Answer» D. Chlorine | |
| 47. | Which of the following is most effective for sterilizing mattresses and plastic petri plates? | 
| A. | Chlorine | 
| B. | Ethylene oxide | 
| C. | Glutaraldehyde | 
| D. | Ultraviolet radiation | 
| Answer» C. Glutaraldehyde | |
| 48. | All of the following are sporicidal except | 
| A. | glutaraldehyde | 
| B. | ethylene oxide | 
| C. | formaldehyde | 
| D. | alcohol | 
| Answer» E. | |
| 49. | Which of the following is bactericidal? | 
| A. | Membrane filtration | 
| B. | Ionizing radiation | 
| C. | Freeze-drying | 
| D. | Deep freezing | 
| Answer» C. Freeze-drying | |
| 50. | Which of the following material is sterilized by heating at 160 C in a hot air oven for one hour? | 
| A. | Swab sticks | 
| B. | All-glass syringes | 
| C. | Oils and jellies | 
| D. | All of these | 
| Answer» E. | |