 
			 
			MCQOPTIONS
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				This section includes 195 Mcqs, each offering curated multiple-choice questions to sharpen your Biochemistry knowledge and support exam preparation. Choose a topic below to get started.
| 1. | In what form does the product of glycolysis enter the TCA cycle? | 
| A. | AcetylCoA | 
| B. | Pyruvate | 
| C. | NADH | 
| D. | Glucose | 
| Answer» B. Pyruvate | |
| 2. | Which of the following takes place in substrate level phosphorylation? | 
| A. | Oxidation of one molecule of substrate is linked to synthesis of more than one ATP molecule | 
| B. | High energy intermediate compounds cannot be isolated | 
| C. | Only mitochondrial reactions participate in ATP formation | 
| D. | Substrate reacts to form a product containing a high energy bond | 
| Answer» E. | |
| 3. | The pigment used in human vision is | 
| A. | Retinal | 
| B. | Vitamin A | 
| C. | Ferredoxin | 
| D. | Beta-carotene | 
| Answer» B. Vitamin A | |
| 4. | The followings are the products of the light reactions of photosynthesis except | 
| A. | ATP | 
| B. | oxygen | 
| C. | NADPH | 
| D. | glucose | 
| Answer» E. | |
| 5. | The regulation of oxidative phosphorylation depends on | 
| A. | Magnitude of ion motive force | 
| B. | Magnitude of electron motive force | 
| C. | Magnitude of proton motive force | 
| D. | None of the mentioned | 
| Answer» D. None of the mentioned | |
| 6. | A water-soluble protein that carries one electron present in mitochondria is | 
| A. | PQʙ | 
| B. | Q | 
| C. | Cytochrome c | 
| D. | Plastocyanin | 
| Answer» D. Plastocyanin | |
| 7. | Carbon dioxide is reduced in | 
| A. | noncyclic photophosphorylation | 
| B. | the Calvin cycle | 
| C. | the light reactions | 
| D. | both light and dark reactions | 
| Answer» C. the light reactions | |
| 8. | Maximum energy per gram on oxidation is yielded from | 
| A. | Starch | 
| B. | Fat | 
| C. | Glycogen | 
| D. | Protein | 
| Answer» C. Glycogen | |
| 9. | Which of the following serves as a reactant in photosynthesis and a product in cellular respiration? | 
| A. | O₂ | 
| B. | CO₂ | 
| C. | Sunlight | 
| D. | ATP | 
| Answer» C. Sunlight | |
| 10. | Oxidative phosphorylation is to respiration as __________ is to photosynthesis | 
| A. | carbon fixation | 
| B. | electron transport chain | 
| C. | light capture by chlorophyll | 
| D. | reduction of NADPH | 
| Answer» C. light capture by chlorophyll | |
| 11. | What process in cellular respiration is essentially the reverse of carbon fixation in photosynthesis? | 
| A. | Glycolysis | 
| B. | Citric acid cycle | 
| C. | Oxidative phosphorylation | 
| D. | Alcohol fermentation | 
| Answer» C. Oxidative phosphorylation | |
| 12. | Which of the following describes the complete route by which electrons flow H₂O to NADP⁺? | 
| A. | Z scheme | 
| B. | A scheme | 
| C. | B scheme | 
| D. | X scheme | 
| Answer» B. A scheme | |
| 13. | Oxidative stress (in humans) is not involved in the development of | 
| A. | Cancer | 
| B. | Parkinson’s disease | 
| C. | Heart failure | 
| D. | Kidney failure | 
| Answer» E. | |
| 14. | What is the proportion of ATP produced by oxidative phosphorylation? | 
| A. | 60% | 
| B. | 70% | 
| C. | 80% | 
| D. | 90% | 
| Answer» B. 70% | |
| 15. | Which one of the following is a product of both cyclic and noncyclic photophosphorylation? | 
| A. | NADPH | 
| B. | O₂ | 
| C. | ATP | 
| D. | Carbohydrate | 
| Answer» D. Carbohydrate | |
| 16. | The end products of noncyclic photophosphorylation are | 
| A. | O₂, ATP and NADPH | 
| B. | carbon dioxide, PGAL (phosphoglyceraldehyde), and H2 | 
| C. | water, ADP and NADP+ | 
| D. | carbon dioxide, ATP and water | 
| Answer» B. carbon dioxide, PGAL (phosphoglyceraldehyde), and H2 | |
| 17. | The effect of increased levels of hydrogen ions in the inter-membrane space of the mitochondria is | 
| A. | Increase ATP production | 
| B. | Decreased levels of oxidative phosphorylation | 
| C. | Increased levels of water in inter-membrane space | 
| D. | Decreased levels of chemiosmosis | 
| Answer» B. Decreased levels of oxidative phosphorylation | |
| 18. | Which of the following accepts only one electron? | 
| A. | Cytochrome b | 
| B. | Coenzyme Q | 
| C. | FMN | 
| D. | FAD | 
| Answer» B. Coenzyme Q | |
| 19. | NADP is a cofactor used in | 
| A. | Catabolic reactions | 
| B. | Anabolic reactions | 
| C. | Elimination reaction | 
| D. | Redox reactions | 
| Answer» C. Elimination reaction | |
| 20. | NADP⁺ in its reduced form is | 
| A. | NAD | 
| B. | NADH | 
| C. | NADPH | 
| D. | None of the mentioned | 
| Answer» D. None of the mentioned | |
| 21. | Which of the following is the prosthetic group of NADH dehydrogenase? | 
| A. | NADH | 
| B. | FAD | 
| C. | NADPH | 
| D. | FMN | 
| Answer» E. | |
| 22. | If rotenone is added to the mitochondrial electron transport chain | 
| A. | Succinate oxidation remains normal | 
| B. | P: O ratio of NADH is reduced from 3:1 to 2:1 | 
| C. | Oxidative phosphorylation is uncoupled at site I | 
| D. | Rate of NADH oxidation is diminished to two-thirds of its initial value | 
| Answer» B. P: O ratio of NADH is reduced from 3:1 to 2:1 | |
| 23. | The transport of acyl co-A for oxidation using a shuttle involves formation of the intermediate | 
| A. | Acyl coenzyme A | 
| B. | 3 acetyl co-A | 
| C. | Acyl cartinine | 
| D. | None of the mentioned | 
| Answer» D. None of the mentioned | |
| 24. | What do NAD+, NADP+, and FAD+ all have in common? | 
| A. | They are reduced | 
| B. | They have a full complement of electrons | 
| C. | They are oxidized | 
| D. | They are what is used during carbon fixation in photosynthesis | 
| Answer» D. They are what is used during carbon fixation in photosynthesis | |
| 25. | The proposal of chemiosmotic hypothesis was by | 
| A. | Peter D. Mitchell | 
| B. | Charles Darwin | 
| C. | Mendele | 
| D. | Alfred Russell | 
| Answer» B. Charles Darwin | |
| 26. | Complex 3 is also called _________ | 
| A. | NADH dehydrogenase | 
| B. | Succinate dehydrogenase | 
| C. | Cytochrome bc1 complex | 
| D. | Cytochrome oxidase | 
| Answer» D. Cytochrome oxidase | |
| 27. | The catabolism of sugars and fatty acids is similar because | 
| A. | both of these compounds are funnelled through the TC A/citric acid cycle | 
| B. | both of these compounds generate redox energy during catabolism | 
| C. | both of these compounds generate chemical energy during catabolism | 
| D. | all of the above | 
| Answer» B. both of these compounds generate redox energy during catabolism | |
| 28. | Where do the protons come from that make up the proton gradient used in the light reactions of photosynthesis? | 
| A. | Glucose | 
| B. | ATP | 
| C. | H₂O | 
| D. | NADPH | 
| Answer» D. NADPH | |
| 29. | A water-soluble protein that carries one electron present in chloroplast is | 
| A. | PQʙ | 
| B. | Q | 
| C. | Cytochrome c | 
| D. | Plastocyanin | 
| Answer» E. | |
| 30. | A mobile, lipid soluble carrier of two electrons present in chloroplasts is | 
| A. | PQʙ | 
| B. | Q | 
| C. | Cytochrome c | 
| D. | Plastocyanin | 
| Answer» B. Q | |
| 31. | One of the following is the source of electrons in photosynthesis | 
| A. | Carbohydrates | 
| B. | CO₂ | 
| C. | Water | 
| D. | NADH | 
| Answer» D. NADH | |
| 32. | Which of the following structures or processes are logically associated with chloroplasts? | 
| A. | Plant cells | 
| B. | Chlorophyll | 
| C. | Thylakoid membranes | 
| D. | All of these | 
| Answer» E. | |
| 33. | Hydrogen is transferred from the light reaction to the dark reaction in photosynthesis by | 
| A. | DPN | 
| B. | NAD | 
| C. | ATP | 
| D. | NADP | 
| Answer» B. NAD | |
| 34. | The size of plant mitochondrial DNA is | 
| A. | 16kb | 
| B. | 200-2500kb | 
| C. | 100- 2550kb | 
| D. | 100-2500kb | 
| Answer» C. 100- 2550kb | |
| 35. | Plastoquinol formed in PSII is oxidized by | 
| A. | Cytochrome b₆f | 
| B. | Cytochrome c | 
| C. | Cytochrome f | 
| D. | None of the mentioned | 
| Answer» B. Cytochrome c | |
| 36. | In algae, photosynthesis takes place in | 
| A. | choloroplasts | 
| B. | cell membrane | 
| C. | mitochondria | 
| D. | none of the above | 
| Answer» B. cell membrane | |
| 37. | Assume the combined processes of photosynthesis and cellular respiration, the electrons that start as part of H2O at the beginning of the light reactions end up attaching to | 
| A. | O₂ to make new H₂O | 
| B. | NADPH to make new glucose | 
| C. | pyruvate to make ethanol | 
| D. | electron transport carriers to make O₂ | 
| Answer» B. NADPH to make new glucose | |
| 38. | Every cycle of β-oxidation produces | 
| A. | 1 FAD, 1 NAD⁺ and 2 CO₂ molecules | 
| B. | 1 FADH₂, 1 NADH and 1 acetyl co-A | 
| C. | 1 FADH₂, 1 NAD⁺ and 1 acetyl co-A | 
| D. | 1 FAD, 1 NADH and 2 CO₂ molecules | 
| Answer» C. 1 FADH₂, 1 NAD⁺ and 1 acetyl co-A | |
| 39. | Which enzyme is involved in carbon-fixation reaction? | 
| A. | NADP reductase | 
| B. | Cytochrome reductase | 
| C. | Ribulose bisphosphate carboxylase | 
| D. | Glycerol kinase | 
| Answer» D. Glycerol kinase | |
| 40. | Calvin cycle occurs in | 
| A. | Cytoplasm | 
| B. | Chloroplast | 
| C. | Mitochondria | 
| D. | Golgi body | 
| Answer» B. Chloroplast | |
| 41. | Complex 2 is also called _________ | 
| A. | NADH dehydrogenase | 
| B. | Succinate dehydrogenase | 
| C. | Cytochrome bc1 complex | 
| D. | Cytochrome oxidase | 
| Answer» C. Cytochrome bc1 complex | |
| 42. | Energy that is released from glucose during respiration but not transferred to ATP bonds can be detected as | 
| A. | CO₂ | 
| B. | AMP | 
| C. | ADP | 
| D. | heat | 
| Answer» E. | |
| 43. | The size of human mitochondrial DNA is | 
| A. | 16kb | 
| B. | 200kb | 
| C. | 2500kb | 
| D. | 100kb | 
| Answer» B. 200kb | |
| 44. | A soluble Cu-containing electron transfer protein is | 
| A. | Pheophytin | 
| B. | Cytochrome c | 
| C. | Plastocyanin | 
| D. | Ferredoxin | 
| Answer» D. Ferredoxin | |
| 45. | During the light-dependent reactions of photosynthesis, which of the following does not occur? | 
| A. | Splitting of water | 
| B. | Carbon dioxide fixation | 
| C. | Release of oxygen | 
| D. | Absorption of light energy by photosystems | 
| Answer» C. Release of oxygen | |
| 46. | Complex 1 is also called _________ | 
| A. | NADH dehydrogenase | 
| B. | Succinate dehydrogenase | 
| C. | Cytochrome bc1 complex | 
| D. | Cytochrome oxidase | 
| Answer» B. Succinate dehydrogenase | |
| 47. | Cholesterol can be synthesized de novo in | 
| A. | pancreas | 
| B. | intestine | 
| C. | liver | 
| D. | cell membrane | 
| Answer» D. cell membrane | |
| 48. | Photosystem II is a | 
| A. | Pheophytin-quinone type | 
| B. | Ferredoxin type | 
| C. | Plastocyanin type | 
| D. | Plastocyanin-ferredoxin type | 
| Answer» B. Ferredoxin type | |
| 49. | Which one of the following is true about C₄ plants? | 
| A. | They minimize rubisco oxygenase activity by fixing CO₂ into oxaloacetate | 
| B. | They mainly occur in areas of high temperature and light intensity | 
| C. | They minimize the rubisco oxygenase activity by fixing CO₂ into phosphoenolpyruvic acid | 
| D. | C₃ plants are more efficient in photosynthesis than C₄ plants | 
| Answer» B. They mainly occur in areas of high temperature and light intensity | |
| 50. | When compared to retinal, chlorophyll can be described as a pigment that has a | 
| A. | Narrow absorption range but high efficiency | 
| B. | Narrow absorption range but low efficiency | 
| C. | Wide absorption range but high efficiency | 
| D. | Wide absorption range but low efficiency | 
| Answer» B. Narrow absorption range but low efficiency | |