Explore topic-wise MCQs in Protein Engineering.

This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Protein Engineering knowledge and support exam preparation. Choose a topic below to get started.

1.

If we start with 10 double-stranded DNA molecules in PCR amplification, then how many molecules of dsDNA would be obtained after 10 cycles of PCR amplification?

A. 10,420
B. 5,120
C. 20,480
D. 10,240
Answer» E.
2.

What is the temperature of the reaction mixture in the extension/elongation step of PCR?

A. 90-94 C
B. 50-65 C
C. 100-110 C
D. 68-72 C
Answer» E.
3.

If we start with a single double-stranded DNA molecule in PCR amplification, then how many molecules of dsDNA would be obtained after 23 cycles of PCR amplification?

A. 23,258,369
B. 5,456,987
C. 7,256,458
D. 8,388,608
Answer» E.
4.

Which of the following cannot produce errors in error-prone PCR?

A. High concentration of dATPs in the reaction mixture
B. High concentration of Mg<sup>+2</sup>
C. High concentration of dTTPs in the reaction mixture
D. Taq polymerase with proof-reading ability
Answer» E.
5.

What is the temperature of the reaction mixture in the second (annealing) step of PCR?

A. 100-110 C
B. 90-94 C
C. 70-72 C
D. 50-65 C
Answer» E.
6.

In error-prone PCR the Taq polymerase anneals incompatible base-pairs to each other during amplification.

A. False
B. True
Answer» C.
7.

Which of the following technique allows, the initiation of DNA amplification, starting with tiny amounts of the parent molecule, and produces considerable amounts of mutated genes?

A. Southern Blotting
B. Western Blotting
C. PCR
D. Error-prone PCR
Answer» E.
8.

What is the temperature of the reaction mixture in the initiation step of PCR?

A. 70-72 C
B. 100-110 C
C. 68-70 C
D. 94-96 C
Answer» E.
9.

PCR machine is also known as thermo-cycler.

A. False
B. True
Answer» C.
10.

Which of the following is not supposed to be present in the PCR reaction mixture solution?

A. Deoxynucleotide triphosphates (dNTPs)
B. Magnesium ions
C. Buffer
D. Calcium ions
Answer» E.
11.

Which of the following component is not required in a basic PCR?

A. DNA template
B. Two primers
C. Thermostable DNA polymerase
D. Initiation and Elongation Factors
Answer» E.
12.

Which of the following technique is used to amplify a precise fragment of DNA from a complex mixture of starting material called template DNA?

A. Northern Blotting
B. Southern Blotting
C. Western Blotting
D. PCR
Answer» E.
13.

Who discovered PCR?

A. Charles Chamberland
B. Linus Pauling
C. Robert brown
D. Kary Mullis
Answer» E.
14.

Directed evolution screens for new enzyme activities by constructing a library of different enzymes derived from the same original protein.

A. False
B. True
Answer» C.
15.

Which of the following technique is used to alter the function of an enzyme without the need for exhaustive structural and functional information?

A. Slow evolution
B. Indirect evolution
C. Rational protein design
D. Directed evolution
Answer» E.