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This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Protein Engineering knowledge and support exam preparation. Choose a topic below to get started.
1. |
If we start with 10 double-stranded DNA molecules in PCR amplification, then how many molecules of dsDNA would be obtained after 10 cycles of PCR amplification? |
A. | 10,420 |
B. | 5,120 |
C. | 20,480 |
D. | 10,240 |
Answer» E. | |
2. |
What is the temperature of the reaction mixture in the extension/elongation step of PCR? |
A. | 90-94 C |
B. | 50-65 C |
C. | 100-110 C |
D. | 68-72 C |
Answer» E. | |
3. |
If we start with a single double-stranded DNA molecule in PCR amplification, then how many molecules of dsDNA would be obtained after 23 cycles of PCR amplification? |
A. | 23,258,369 |
B. | 5,456,987 |
C. | 7,256,458 |
D. | 8,388,608 |
Answer» E. | |
4. |
Which of the following cannot produce errors in error-prone PCR? |
A. | High concentration of dATPs in the reaction mixture |
B. | High concentration of Mg<sup>+2</sup> |
C. | High concentration of dTTPs in the reaction mixture |
D. | Taq polymerase with proof-reading ability |
Answer» E. | |
5. |
What is the temperature of the reaction mixture in the second (annealing) step of PCR? |
A. | 100-110 C |
B. | 90-94 C |
C. | 70-72 C |
D. | 50-65 C |
Answer» E. | |
6. |
In error-prone PCR the Taq polymerase anneals incompatible base-pairs to each other during amplification. |
A. | False |
B. | True |
Answer» C. | |
7. |
Which of the following technique allows, the initiation of DNA amplification, starting with tiny amounts of the parent molecule, and produces considerable amounts of mutated genes? |
A. | Southern Blotting |
B. | Western Blotting |
C. | PCR |
D. | Error-prone PCR |
Answer» E. | |
8. |
What is the temperature of the reaction mixture in the initiation step of PCR? |
A. | 70-72 C |
B. | 100-110 C |
C. | 68-70 C |
D. | 94-96 C |
Answer» E. | |
9. |
PCR machine is also known as thermo-cycler. |
A. | False |
B. | True |
Answer» C. | |
10. |
Which of the following is not supposed to be present in the PCR reaction mixture solution? |
A. | Deoxynucleotide triphosphates (dNTPs) |
B. | Magnesium ions |
C. | Buffer |
D. | Calcium ions |
Answer» E. | |
11. |
Which of the following component is not required in a basic PCR? |
A. | DNA template |
B. | Two primers |
C. | Thermostable DNA polymerase |
D. | Initiation and Elongation Factors |
Answer» E. | |
12. |
Which of the following technique is used to amplify a precise fragment of DNA from a complex mixture of starting material called template DNA? |
A. | Northern Blotting |
B. | Southern Blotting |
C. | Western Blotting |
D. | PCR |
Answer» E. | |
13. |
Who discovered PCR? |
A. | Charles Chamberland |
B. | Linus Pauling |
C. | Robert brown |
D. | Kary Mullis |
Answer» E. | |
14. |
Directed evolution screens for new enzyme activities by constructing a library of different enzymes derived from the same original protein. |
A. | False |
B. | True |
Answer» C. | |
15. |
Which of the following technique is used to alter the function of an enzyme without the need for exhaustive structural and functional information? |
A. | Slow evolution |
B. | Indirect evolution |
C. | Rational protein design |
D. | Directed evolution |
Answer» E. | |