MCQOPTIONS
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MCQOPTIONS
This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Protein Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
The three steps of denaturation, annealing, and polymerase extension are repeated for multiple cycles. |
| A. | False |
| B. | True |
| Answer» C. | |
| 2. |
Which step comes after DNA fragmentation in DNA shuffling technique? |
| A. | Polymerase extension |
| B. | Annealing |
| C. | DNase I treatment |
| D. | Denaturation |
| Answer» E. | |
| 3. |
The fragmentation of DNA in the DNA shuffling technique occurs at specific sites in DNA. |
| A. | True |
| B. | False |
| Answer» C. | |
| 4. |
Which of the following can be used to create random fragments of DNA? |
| A. | DNA ligase |
| B. | EcoR I |
| C. | BamH I |
| D. | DNase I |
| Answer» E. | |
| 5. |
Which of the following is not a step of the DNA shuffling technique? |
| A. | Polymerase extension |
| B. | Denaturation |
| C. | Annealing |
| D. | Phage display |
| Answer» E. | |
| 6. |
DNA shuffling is also known as sexual PCR. |
| A. | False |
| B. | True |
| Answer» C. | |
| 7. |
Which of the following is a method to recombine fragments generated in DNA shuffling? |
| A. | RT-PCR |
| B. | Error-prone PCR |
| C. | Random PCR |
| D. | Overlap PCR |
| Answer» E. | |
| 8. |
Which step is performed before the partial digestion of the library of genes by restriction enzymes? |
| A. | Reassembly |
| B. | X-ray crystallography |
| C. | Protein assay |
| D. | Random mutagenesis |
| Answer» E. | |
| 9. |
Which of the following is not true for DNA shuffling? |
| A. | It involves random DNA fragmentation |
| B. | It recombines beneficial mutations from different molecules |
| C. | It involves many cycles of PCR |
| D. | It involves the addition of primers |
| Answer» E. | |
| 10. |
A more powerful variant of DNA polymerase is to start with several closely related (i.e. homologous) versions of the same gene from different organisms. |
| A. | False |
| B. | True |
| Answer» C. | |
| 11. |
In DNA shuffling the gene to be improved is cut into random segments. What is the length of these segments? |
| A. | 10-50 base pairs long |
| B. | 2-10 base pairs long |
| C. | 500-1000 base pairs long |
| D. | 100-300 base pairs long |
| Answer» E. | |
| 12. |
Which of the following is a method of artificial evolution achieved by reassembling different fragments and includes the creation of novel mutations as well as recombination? |
| A. | Phage display |
| B. | Rational protein design |
| C. | Error-prone PCR |
| D. | DNA shuffling |
| Answer» E. | |
| 13. |
What happens with the length of fragments in each PCR cycle? |
| A. | It can either increase or decrease |
| B. | It decreases |
| C. | It remains constant |
| D. | It increases |
| Answer» E. | |
| 14. |
Irrational protein design is also known as directed evolution. |
| A. | False |
| B. | True |
| Answer» C. | |
| 15. |
Which of the following strategy of protein engineering is based on high throughput protein engineering? |
| A. | Slow evolution |
| B. | Rational evolution |
| C. | Natural evolution |
| D. | Irrational protein design |
| Answer» E. | |