MCQOPTIONS
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MCQOPTIONS
This section includes 14 Mcqs, each offering curated multiple-choice questions to sharpen your Protein Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Which of the following is a correct feature for the free-interface diffusion method of protein crystallization? |
| A. | Only one layer of precipitant solution |
| B. | Only one layer of protein solution |
| C. | The upper layer of a precipitant solution and lower layer of protein solution |
| D. | The upper layer of protein solution and lower layer of precipitant solution |
| Answer» E. | |
| 2. |
Which of the following method uses different conditions for nucleation and growth to achieve improved crystal quality? |
| A. | Vapour diffusion |
| B. | Microdialysis |
| C. | Free-interface diffusion |
| D. | Micro-batch crystallization |
| Answer» E. | |
| 3. |
In which of the following method, the droplet must be covered with paraffin oil to prevent evaporation? |
| A. | Microdialysis |
| B. | Free-interface diffusion |
| C. | Vapour diffusion |
| D. | Micro-batch crystallization |
| Answer» E. | |
| 4. |
Which of the following method utilizes free diffusion between a concentrated protein solution and a precipitant solution? |
| A. | Micro-batch crystallization |
| B. | Vapour diffusion |
| C. | Microdialysis |
| D. | Free-interface diffusion |
| Answer» E. | |
| 5. |
Which of the following method takes advantage of a semi-permeable membrane for protein crystallization? |
| A. | Vapour diffusion |
| B. | Micro-batch crystallization |
| C. | Free-interface diffusion |
| D. | Microdialysis |
| Answer» E. | |
| 6. |
In which of the following method, the protein drop is on the elevated platform? |
| A. | Hanging-drop vapour diffusion method |
| B. | Elevated-drop vapour diffusion method |
| C. | Standing-drop vapour diffusion method |
| D. | Sitting-drop vapour diffusion method |
| Answer» E. | |
| 7. |
In the sitting drop vapour diffusion method the protein drop is suspended from a coverslip over the reservoir. |
| A. | True |
| B. | False |
| Answer» C. | |
| 8. |
Which of the following does not make the protein solution supersaturated? |
| A. | Addition of salt |
| B. | Change of pH |
| C. | Addition of bridging ions |
| D. | Addition of water |
| Answer» E. | |
| 9. |
Which of the following does not promote protein crystal formation? |
| A. | Addition of organic solvents |
| B. | Direct mixing of protein and precipitant solutions |
| C. | Altering the temperature |
| D. | Addition of a solubilizing agent |
| Answer» E. | |
| 10. |
Which of the following is not a method for creating supersaturation? |
| A. | Salting in |
| B. | Salting out |
| C. | Evaporation |
| D. | Dilution |
| Answer» E. | |
| 11. |
Nucleation represents which order phase transition? |
| A. | Zero-order |
| B. | Third-order |
| C. | Second-order |
| D. | First-order |
| Answer» E. | |
| 12. |
Crystallization of proteins proceeds in two distinct steps. Which are these steps? |
| A. | Solidification and crystallization |
| B. | Crystallization and expansion |
| C. | Growth and solidification |
| D. | Nucleation and growth |
| Answer» E. | |
| 13. |
The extent of the diffraction pattern from a crystal is inversely correlated with its degree of internal order. |
| A. | True |
| B. | False |
| Answer» C. | |
| 14. |
Macromolecular crystals are temperature sensitive. |
| A. | False |
| B. | True |
| Answer» C. | |