Explore topic-wise MCQs in Molecular Biology.

This section includes 10 Mcqs, each offering curated multiple-choice questions to sharpen your Molecular Biology knowledge and support exam preparation. Choose a topic below to get started.

1.

After the binding of polymerase to the DNA template the σ factor is ejected.

A. True
B. False
Answer» C.
Discussion
2.

The ternary complex is formed by the polymerase, DNA duplex and the nascent RNA.

A. True
B. False
Answer» C.
Discussion
3.

RNA polymerase adds the nucleotides de novo. The first nucleotide added by the polymerase is ____________

A. ATP
B. TTP
C. GTP
D. CTP
Answer» B. TTP
Discussion
4.

Region 1.1 of the σ factor mimics the DNA and is negatively charged.

A. True
B. False
Answer» B. False
Discussion
5.

The channel that allows the exit of the coding strand is known as ____________

A. NTP channel
B. NT channel
C. T channel
D. Downstream DNA channel
Answer» C. T channel
Discussion
6.

How many channels are found in the RNA polymerase clamp?

A. 3
B. 4
C. 5
D. 6
Answer» D. 6
Discussion
7.

With respect to the formation of a complex between polymerase and DNA template which of the following is correct?

A. Open: reversible; Closed: irreversible
B. Open: irreversible; Closed: reversible
C. Open and closed: reversible
D. Open and closed: irreversible
Answer» C. Open and closed: reversible
Discussion
8.

The isomerisation of σ70 of bacterial polymerase is an energy dependent process.

A. True
B. False
Answer» C.
Discussion
9.

To facilitate binding of σ70 the melting of the template and non – template strands occurs between the positions ____________

A. -11 to +3
B. -9 to +5
C. -10 to +1
D. -10 to +2
Answer» B. -9 to +5
Discussion
10.

Without using primers for RNA synthesis the polymerase produces small stretches of RNA of _____________ nucleotides.

A. 8
B. 10
C. 12
D. 14
Answer» C. 12
Discussion