Explore topic-wise MCQs in Molecular Biology.

This section includes 14 Mcqs, each offering curated multiple-choice questions to sharpen your Molecular Biology knowledge and support exam preparation. Choose a topic below to get started.

1.

The process by which every type of transformant can be identified is __________

A. Replica plating
B. Hybridization
C. Blotting
D. Insertional inactivation
Answer» E.
2.

Under the influence of which ion does E. coli takes up plasmid from the environment?

A. Nickel
B. Copper
C. Lead
D. Sulphur
Answer» C. Lead
3.

The vector and the DNA insert are cut by different enzymes for convenience.

A. True
B. False
Answer» C.
4.

DNA ligase can ligate restriction site ends produced by EcoRI to the ends of DNA insert cut by the same enzyme.

A. True
B. False
Answer» B. False
5.

UNDER_THE_INFLUENCE_OF_WHICH_ION_DOES_E._COLI_TAKES_UP_PLASMID_FROM_THE_ENVIRONMENT??$

A. Nickel
B. Copper
C. Lead
D. Sulphur
Answer» C. Lead
6.

The_process_by_which_every_type_of_transformant_can_be_identified_is___________$

A. Replica plating
B. Hybridization
C. Blotting
D. Insertional inactivation
Answer» E.
7.

Which of the following is the primary use of an expression vector?

A. DNA library
B. DNA purification
C. Protein production
D. DNA cloning
Answer» D. DNA cloning
8.

What is the major difference between cloning vectors and primary vectors?

A. Selectable marker
B. DNA inserts
C. Presence of promoter
D. Presence of two Ori
Answer» D. Presence of two Ori
9.

The vector and the DNA insert are cut by different enzymes for convenience __________

A. True
B. False
Answer» C.
10.

DNA ligase can ligate restriction site ends produced by EcoRI to the ends of DNA insert cut by the same enzyme _________

A. True
B. False
Answer» B. False
11.

Which of the following is an essential feature for being a perfect vector?

A. Origin of replication
B. Selectable marker
C. Restriction site
D. Virulent gene
Answer» E.
12.

Which is the most common organism considered for genetic manipulations?

A. E. coli
B. Saccharomyces cerevisiae
C. Cyanobacteria
D. Bacillus Subtilis
Answer» B. Saccharomyces cerevisiae
13.

Transformation does not involve ____________

A. Cutting
B. Recombination
C. Propagation
D. Expression
Answer» E.
14.

During DNA cloning which of the following is not a crucial requirement?

A. DNA inserts
B. Vector
C. Protein expression
D. Molecular cutter
Answer» D. Molecular cutter