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This section includes 14 Mcqs, each offering curated multiple-choice questions to sharpen your Molecular Biology knowledge and support exam preparation. Choose a topic below to get started.
1. |
The process by which every type of transformant can be identified is __________ |
A. | Replica plating |
B. | Hybridization |
C. | Blotting |
D. | Insertional inactivation |
Answer» E. | |
2. |
Under the influence of which ion does E. coli takes up plasmid from the environment? |
A. | Nickel |
B. | Copper |
C. | Lead |
D. | Sulphur |
Answer» C. Lead | |
3. |
The vector and the DNA insert are cut by different enzymes for convenience. |
A. | True |
B. | False |
Answer» C. | |
4. |
DNA ligase can ligate restriction site ends produced by EcoRI to the ends of DNA insert cut by the same enzyme. |
A. | True |
B. | False |
Answer» B. False | |
5. |
UNDER_THE_INFLUENCE_OF_WHICH_ION_DOES_E._COLI_TAKES_UP_PLASMID_FROM_THE_ENVIRONMENT??$ |
A. | Nickel |
B. | Copper |
C. | Lead |
D. | Sulphur |
Answer» C. Lead | |
6. |
The_process_by_which_every_type_of_transformant_can_be_identified_is___________$ |
A. | Replica plating |
B. | Hybridization |
C. | Blotting |
D. | Insertional inactivation |
Answer» E. | |
7. |
Which of the following is the primary use of an expression vector? |
A. | DNA library |
B. | DNA purification |
C. | Protein production |
D. | DNA cloning |
Answer» D. DNA cloning | |
8. |
What is the major difference between cloning vectors and primary vectors? |
A. | Selectable marker |
B. | DNA inserts |
C. | Presence of promoter |
D. | Presence of two Ori |
Answer» D. Presence of two Ori | |
9. |
The vector and the DNA insert are cut by different enzymes for convenience __________ |
A. | True |
B. | False |
Answer» C. | |
10. |
DNA ligase can ligate restriction site ends produced by EcoRI to the ends of DNA insert cut by the same enzyme _________ |
A. | True |
B. | False |
Answer» B. False | |
11. |
Which of the following is an essential feature for being a perfect vector? |
A. | Origin of replication |
B. | Selectable marker |
C. | Restriction site |
D. | Virulent gene |
Answer» E. | |
12. |
Which is the most common organism considered for genetic manipulations? |
A. | E. coli |
B. | Saccharomyces cerevisiae |
C. | Cyanobacteria |
D. | Bacillus Subtilis |
Answer» B. Saccharomyces cerevisiae | |
13. |
Transformation does not involve ____________ |
A. | Cutting |
B. | Recombination |
C. | Propagation |
D. | Expression |
Answer» E. | |
14. |
During DNA cloning which of the following is not a crucial requirement? |
A. | DNA inserts |
B. | Vector |
C. | Protein expression |
D. | Molecular cutter |
Answer» D. Molecular cutter | |