MCQOPTIONS
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MCQOPTIONS
This section includes 22 Mcqs, each offering curated multiple-choice questions to sharpen your Biochemistry knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Immunoaffinity chromatography can be used in biochemical applications to |
| A. | break down antibody structure |
| B. | purify protein antigen |
| C. | break down antigen and analyze quantitatively |
| D. | none of the above |
| Answer» C. break down antigen and analyze quantitatively | |
| 2. |
What is the ELISA test intended to measure? |
| A. | Antibody to HIV only |
| B. | Antigen to HIV only |
| C. | Presence of free, circulating virus in the patient |
| D. | Antibodies directed against HLA molecules |
| Answer» B. Antigen to HIV only | |
| 3. |
Different form of a plasmid, on an agarose gel, after electrophoresis may be in which of the following sequence in decreasing molecular weight? |
| A. | Open circular, super coiled, linear plasmid DNA |
| B. | Linear plasmid, open circular, super coiled plasmid |
| C. | Open circular, super coiled, linear plasmid DNA |
| D. | Open circular, linear plasmid, super coiled plasmid |
| Answer» C. Open circular, super coiled, linear plasmid DNA | |
| 4. |
Radial Immunodiffusion is similar to |
| A. | double-diffusion |
| B. | gel diffusion |
| C. | Ouchterloney technique |
| D. | all of these |
| Answer» B. gel diffusion | |
| 5. |
Agarose gel electrophoresis and pulsed field gel electrophoresis may be used to resolve respectively |
| A. | 2000 kb and 20kb DNA |
| B. | 1000 kb and l0kb DNA |
| C. | 20 kb and 2000 kb DNA |
| D. | 10 kb and 1000 kb DNA |
| Answer» D. 10 kb and 1000 kb DNA | |
| 6. |
The IPTG is a gratuitous inducers of ( -galactosidase, and x-gal is a chromogenic substrate. When -complementation occurs which colour is produced? |
| A. | Blue |
| B. | White |
| C. | Brown |
| D. | No colour is produced |
| Answer» B. White | |
| 7. |
The competitive immunoassay can be used |
| A. | to detect very small amounts of antigen |
| B. | to detect antibody associated with allergies (IgE) |
| C. | both (a) and (b) |
| D. | commonly to detect trace amounts of drugs. |
| Answer» D. commonly to detect trace amounts of drugs. | |
| 8. |
In Counter immunoelectrophoresis |
| A. | electrophoresis will drive the antibody and antigen parallel to each other |
| B. | electrophoresis will drive the antibody and antigen toward each other |
| C. | the antibody will migrate towards anode |
| D. | the antibody will migrate towards cathode |
| Answer» C. the antibody will migrate towards anode | |
| 9. |
Which of the following is an immunodiffusion test? |
| A. | Double-diffusion |
| B. | Gel diffusion |
| C. | Ouchterloney technique |
| D. | All of these |
| Answer» E. | |
| 10. |
Immunoelectrophoresis techniques are designed to separate the mixture components from each other by using electrophoresis |
| A. | prior to reaction with antibody |
| B. | prior to reaction with antigen |
| C. | after reaction with antibody |
| D. | after reaction with antigen |
| Answer» B. prior to reaction with antigen | |
| 11. |
What is approximatively the half life of 32-Phosphorus? |
| A. | 14 days |
| B. | 28 days |
| C. | 42 days |
| D. | 30 days |
| Answer» B. 28 days | |
| 12. |
Which of the following is a positively charged matrix for ion exchange chromatography? |
| A. | CM cellulose |
| B. | DEAE cellulose |
| C. | Phosphocellulose |
| D. | None of these |
| Answer» C. Phosphocellulose | |
| 13. |
RAST test (radio allergosorbent test) is often used to detect |
| A. | antibodies associated with allergies (IgE) |
| B. | antigen associated with allergies (IgE) |
| C. | bacteriophages |
| D. | None of the above |
| Answer» B. antigen associated with allergies (IgE) | |
| 14. |
Which of the following is an immunoelectrophoresis technique? |
| A. | Two-dimensional immunoelectrophoresis |
| B. | Counterimmunoelectrophoresis |
| C. | Coimmunoelectrophoresis |
| D. | Both (a) and (b) |
| Answer» E. | |
| 15. |
What would happen if the anti-human Ig-conjugate is not washed free of the well before the substrate is added? |
| A. | The ELISA would not develop when the substrate was added |
| B. | The ELISA would develop normally |
| C. | All wells would show uniform over-development due to unbound and excess anti-human Ig enzyme conjugate |
| D. | None of the above |
| Answer» D. None of the above | |
| 16. |
Which of the following radioactive element is generally involved in RIA? |
| A. | Tritium |
| B. | Carbon-14 |
| C. | Iodine-125 |
| D. | All of these |
| Answer» E. | |
| 17. |
Agarose a porous matrix used in various molecular biology technique is made by repetitive units of |
| A. | maltose |
| B. | agarobiose |
| C. | agar |
| D. | none of these |
| Answer» B. agarobiose | |
| 18. |
In Rocket Immunodiffusion the length of the rocket is |
| A. | proportional to the amount of antibody placed in each well |
| B. | inversely proportional to the amount of antibody placed in each well |
| C. | inversely proportional to the amount of antigen placed in each well |
| D. | proportional to the amount of antigen placed in each well |
| Answer» E. | |
| 19. |
Rocket Immunodiffusion is also known as |
| A. | gel diffusion |
| B. | electroimmunodiffusion |
| C. | double-diffusion |
| D. | none of these |
| Answer» C. double-diffusion | |
| 20. |
An antigen preparation and an antibody preparation are tested by immunodiffusion. Three bands are found, indicating that |
| A. | there was more than one antigen |
| B. | the antibody was impure |
| C. | the temperature was too high |
| D. | there was one antibody and one antigen |
| Answer» B. the antibody was impure | |
| 21. |
DNA- footprinting is a suitable technique for identifying which of the following? |
| A. | Particular mRNA in mixture |
| B. | Particular t-RNA in mixture |
| C. | Introns within DNA |
| D. | Protein binding site within DNA |
| Answer» E. | |
| 22. |
What would happen if serum is omitted from the ELISA, but all other steps remain same? |
| A. | Anti-human Ig-conjugate would not bind and be washed away |
| B. | The OD values would be nearly the same as the assay control |
| C. | Both (a) and (b) |
| D. | Anti-human Ig-conjugate would bind non-specifically to the ELISA plate |
| Answer» D. Anti-human Ig-conjugate would bind non-specifically to the ELISA plate | |