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This section includes 8 Mcqs, each offering curated multiple-choice questions to sharpen your Molecular Biology knowledge and support exam preparation. Choose a topic below to get started.
1. |
The repressor for the -galactosidase gene is encoded by ___________ |
A. | XmaI |
B. | PstI |
C. | Lac I |
D. | PvuII |
Answer» D. PvuII | |
2. |
Which one of the following is the first engineered plasmid vector? |
A. | pBR322 |
B. | pBR320 |
C. | pUC18 |
D. | pSC101 |
Answer» E. | |
3. |
What is the characteristic of lacZ gene of pUC18 vector among the following? |
A. | Encodes for antibiotic resistance |
B. | Encodes for -galactosidase enzyme |
C. | Encodes for -lactamase enzyme |
D. | Encodes for -galactoside transferase enzyme. |
Answer» C. Encodes for -lactamase enzyme | |
4. |
Which gene in the pUC18 vector confers antibiotic resistance to the transformed cells? |
A. | LacZ |
B. | PvuII |
C. | AmpR |
D. | AntII |
Answer» D. AntII | |
5. |
What is the temperature at which bacteria can takes up the plasmid? |
A. | 42 C |
B. | -42 C |
C. | 40 C |
D. | -40 C |
Answer» C. 40 C | |
6. |
The host bacterium takes up a plasmid in presence of ______________ |
A. | Monovalent cations |
B. | Monovalent anions |
C. | Divalent cations |
D. | Divalent anions |
Answer» D. Divalent anions | |
7. |
What is the length of the polylinker segment of plasmid? |
A. | Less than 100 base pair |
B. | Less than 10 base pair |
C. | Less than 70 base pair |
D. | Less than 50 base pair |
Answer» B. Less than 10 base pair | |
8. |
Under relaxed conditions how many copies of plasmid are present in the cell? |
A. | 10 100 copies |
B. | 100 500 copies |
C. | 1 300 copies |
D. | 10 700 copies |
Answer» E. | |