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This section includes 11 Mcqs, each offering curated multiple-choice questions to sharpen your Molecular Biology knowledge and support exam preparation. Choose a topic below to get started.
1. |
The position of band during electrophoresis indicates the dNTP used as a chain terminator in that mixture. |
A. | True |
B. | False |
Answer» C. | |
2. |
Strand separation for DNA sequencing is done to ____________ |
A. | sort the fragments according to their size |
B. | sort the fragments according to their molecular weight |
C. | obtain consecutive sequence as per termination |
D. | obtain specific lanes of sequencing |
Answer» D. obtain specific lanes of sequencing | |
3. |
The four reaction mixtures that were prepared, contained ____________ |
A. | dCTP, dTTP, dGTP, dATP |
B. | dCTP, ddTTP, dGTP, ddATP |
C. | ddCTP, dTTP, ddGTP, dATP |
D. | dCTP, ddTTP, dGTP, dATP |
Answer» E. | |
4. |
With respect to Sanger s enzymatic method of sequencing pick the odd one out. |
A. | Radioactive dideoxyribonucleotides |
B. | Primers |
C. | Klenow fragment |
D. | Restriction digestion |
Answer» E. | |
5. |
Which enzyme is used for the replication in case of Sanger s method of sequencing? |
A. | Polymerase I |
B. | Smaller subunit polymerase I |
C. | Polymerase III |
D. | Larger subunit polymerase I |
Answer» E. | |
6. |
The dideoxy method is also known as _____________ |
A. | Maxem and Gilbert method |
B. | Autosequencing |
C. | Sanger s enzymatic sequencing |
D. | Pyrosequencing |
Answer» D. Pyrosequencing | |
7. |
The process of electrophoresis is the key to sequencing. |
A. | True |
B. | False |
Answer» B. False | |
8. |
Which step is not involved in base-specific cleavage of DNA fragment? |
A. | Modification of concerned base |
B. | Removal of modified base from DNA strand |
C. | Induction of random strand break |
D. | End labeled DNA fragments of variable lengths produced |
Answer» D. End labeled DNA fragments of variable lengths produced | |
9. |
What are the basic base-specific cleavage sites used in Maxem and Gilbert method? |
A. | A, T, G, C |
B. | C, T, A+G, T+C |
C. | A, G, A+T, G+C |
D. | G, C, A+G, C+T |
Answer» E. | |
10. |
The 32P is added at the 3 end by polynucleotidyl kinase. |
A. | True |
B. | False |
Answer» C. | |
11. |
End labeled DNA sequencing is known as dideoxy method of sequencing. |
A. | True |
B. | False |
Answer» C. | |