Explore topic-wise MCQs in Molecular Biology.

This section includes 11 Mcqs, each offering curated multiple-choice questions to sharpen your Molecular Biology knowledge and support exam preparation. Choose a topic below to get started.

1.

The position of band during electrophoresis indicates the dNTP used as a chain terminator in that mixture.

A. True
B. False
Answer» C.
2.

Strand separation for DNA sequencing is done to ____________

A. sort the fragments according to their size
B. sort the fragments according to their molecular weight
C. obtain consecutive sequence as per termination
D. obtain specific lanes of sequencing
Answer» D. obtain specific lanes of sequencing
3.

The four reaction mixtures that were prepared, contained ____________

A. dCTP, dTTP, dGTP, dATP
B. dCTP, ddTTP, dGTP, ddATP
C. ddCTP, dTTP, ddGTP, dATP
D. dCTP, ddTTP, dGTP, dATP
Answer» E.
4.

With respect to Sanger s enzymatic method of sequencing pick the odd one out.

A. Radioactive dideoxyribonucleotides
B. Primers
C. Klenow fragment
D. Restriction digestion
Answer» E.
5.

Which enzyme is used for the replication in case of Sanger s method of sequencing?

A. Polymerase I
B. Smaller subunit polymerase I
C. Polymerase III
D. Larger subunit polymerase I
Answer» E.
6.

The dideoxy method is also known as _____________

A. Maxem and Gilbert method
B. Autosequencing
C. Sanger s enzymatic sequencing
D. Pyrosequencing
Answer» D. Pyrosequencing
7.

The process of electrophoresis is the key to sequencing.

A. True
B. False
Answer» B. False
8.

Which step is not involved in base-specific cleavage of DNA fragment?

A. Modification of concerned base
B. Removal of modified base from DNA strand
C. Induction of random strand break
D. End labeled DNA fragments of variable lengths produced
Answer» D. End labeled DNA fragments of variable lengths produced
9.

What are the basic base-specific cleavage sites used in Maxem and Gilbert method?

A. A, T, G, C
B. C, T, A+G, T+C
C. A, G, A+T, G+C
D. G, C, A+G, C+T
Answer» E.
10.

The 32P is added at the 3 end by polynucleotidyl kinase.

A. True
B. False
Answer» C.
11.

End labeled DNA sequencing is known as dideoxy method of sequencing.

A. True
B. False
Answer» C.