Explore topic-wise MCQs in Polymerase Chain Reaction.

This section includes 29 Mcqs, each offering curated multiple-choice questions to sharpen your Polymerase Chain Reaction knowledge and support exam preparation. Choose a topic below to get started.

1.

Why are vent polymerase and Pfu more efficient than the Taq polymerase?
A. Because of proofreading activity
B. Because of more efficient polymerase activity
C. Both a and b
D. None of the above
Answer» B. Because of more efficient polymerase activity
Discussion
2.

Which of the following is the basic requirement of PCR reaction?
A. Two oligonucleotide primers
B. DNA segment to be amplified
C. A heat-stable DNA polymerase
D. All of the above
Answer» E.
Discussion
3.

Thermus aquatics is the source of _________.
A. Vent polymerase
B. Primase enzyme
C. Taq polymerase
D. Both a and c
Answer» D. Both a and c
Discussion
4.

Which of the following is not a thermostable polymerase?
A. pfu polymerase
B. Taq polymerase
C. Vent polymerase
D. DNA polymerase III
Answer» E.
Discussion
5.

Which of the following statements is accurate for the PCR polymerase chain reaction?
A. Automated PCR machines are called thermal cyclers
B. A thermostable DNA polymerase is required
C. Millions to billions of desired DNA copies can be produced from microgram quantities of DNA
D. All of the above
Answer» E.
Discussion
6.

The polymerase chain reaction is_________.
A. It is a DNA sequencing technique.
B. It is a DNA degradation technique
C. It is a DNA amplification technique
D. All of the above
Answer» D. All of the above
Discussion
7.

The PCR technique was developed by_________.
A. Kohler
B. Altman
C. Milstein
D. Kary Mullis
Answer» E.
Discussion
8.

How many DNA duplex is obtained from one DNA duplex after 4 cycles of PCR?
A. 4
B. 8
C. 16
D. 32
Answer» D. 32
Discussion
9.

Polymerase used for PCR is extracted from _____________
A. Escherichia coli
B. Homo sapiens
C. Thermus aquaticus
D. Saccharomyces cerevisiae
Answer» D. Saccharomyces cerevisiae
Discussion
10.

PCR is a DNA amplifying in vivo method.
A. True
B. False
Answer» C.
Discussion
11.

Primer used for the process of polymerase chain reaction are ______________
A. Single stranded DNA oligonucleotide
B. Double stranded DNA oligonucleotide
C. Single stranded RNA oligonucleotide
D. Double stranded RNA oligonucleotide
Answer» B. Double stranded DNA oligonucleotide
Discussion
12.

Annealing of primer is facilitated by complementary region.
A. True
B. False
Answer» B. False
Discussion
13.

Which of the following is a mismatch?
A. Polymerase Taq polymerase
B. Template double stranded DNA
C. Primer oligonucleotide
D. Synthesis 5 to 3 direction
Answer» C. Primer oligonucleotide
Discussion
14.

The polymer chain reaction is used for_________.
A. Amplifying gene of interest
B. Constructing RAPD maps
C. Detecting the presence of the transgene in an organism
D. All of the above
Answer» E.
Discussion
15.

Which of the following is true for asymmetric PCR?
A. Used for generating double-stranded copies for DNA sequence
B. Used for generating single-stranded copies for DNA sequence
C. Both a and b
D. None of the above
Answer» C. Both a and b
Discussion
16.

Which of the following is an application of polymer chain reaction?
A. Site-directed mutagenesis
B. Site-specific recombination
C. Site-specific translocation
D. All of the above
Answer» B. Site-specific recombination
Discussion
17.

Reverse transcription PCR uses _________.
A. RNA as a template to form DNA
B. mRNA as a template to form cDNA
C. DNA as a template to form ssDNA
D. All of the above
Answer» C. DNA as a template to form ssDNA
Discussion
18.

How many DNA duplexes are obtained from one DNA duplex after 4 cycles of PCR?
A. 8
B. 4
C. 32
D. 16
Answer» E.
Discussion
19.

At what temperature does annealing of DNA and primer take place?
A. 54 C
B. 96 C
C. 42 C
D. 74 C
Answer» B. 96 C
Discussion
20.

Primers used for the process of polymerase chain reaction are _________.
A. Single-stranded RNA oligonucleotide
B. Single-stranded DNA oligonucleotide
C. Double-stranded RNA oligonucleotide
D. Single-stranded DNA oligonucleotide
Answer» C. Double-stranded RNA oligonucleotide
Discussion
21.

At what temperature does denaturation of DNA double helix takes place?
A. 54 C
B. 74 C
C. 94 C
D. 60 C
Answer» D. 60 C
Discussion
22.

Polymerase used for PCR is extracted from _____________ .
A. Homo sapiens
B. Thermus aquaticus
C. Escherichia coli
D. Saccharomyces cerevisiae
Answer» C. Escherichia coli
Discussion
23.

Which of the following statements are true regarding PCR?
A. Primer extension occurs at 72 C
B. Denaturation involves heating at 90 to 98 C
C. Annealing involves the binding of primer between 40 to 60C C
D. All of the above
Answer» E.
Discussion
24.

Denaturation is the process of _________.
A. Heating between 72 C
B. Heating between 40 to 60 C
C. Heating between 90 to 98 C
D. None of the above
Answer» D. None of the above
Discussion
25.

What is the process of binding of primer to the denatured strand called?
A. Annealing
B. Renaturation
C. Denaturation
D. None of the above
Answer» B. Renaturation
Discussion
26.

Which of the following is the first and the most important step in the polymerase chain reaction?
A. Annealing
B. Primer extension
C. Denaturation
D. None of the above
Answer» D. None of the above
Discussion
27.

At what temperature do annealing of DNA and primer takes place?
A. 42
B. 54
C. 74
D. 96
Answer» E.
Discussion
28.

The temperature at which DNA synthesis takes place is 74 C.
A. True
B. False
Answer» B. False
Discussion
29.

At what temperature do denaturation of DNA double helix takes place?
A. 60
B. 54
C. 74
D. 94
Answer» E.
Discussion