MCQOPTIONS
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MCQOPTIONS
This section includes 41 Mcqs, each offering curated multiple-choice questions to sharpen your Cell Cultivations knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Vero cell line is obtained from Kidney cells of |
| A. | African green monkey |
| B. | Chinese hamster |
| C. | Calf |
| D. | Swine |
| Answer» B. Chinese hamster | |
| 2. |
Which of the following is easy and rapid method to interpret viability of cells in culture system |
| A. | Trypan blue dye exclusion |
| B. | Neutral red assay |
| C. | Fluorescein dye assay |
| D. | All of the following |
| Answer» B. Neutral red assay | |
| 3. |
Which of the following virus is used for cell fusion |
| A. | Sendai virus |
| B. | Herpes virus |
| C. | Myxovirus |
| D. | All of the following |
| Answer» E. | |
| 4. |
The process of dedifferentiation in cell culture can give rise to ________________________ |
| A. | induced-pluripotent stem cells |
| B. | carcinoma cells |
| C. | single protoplasts |
| D. | fused protoplasts |
| Answer» B. carcinoma cells | |
| 5. |
Under favorable conditions, the protoplasts can grow into a ____________________ |
| A. | callus culture |
| B. | organ culture |
| C. | root culture |
| D. | shoot culture |
| Answer» B. organ culture | |
| 6. |
HeLa cells are a cell line. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 7. |
EDTA binds the ______________ ions. |
| A. | magnesium |
| B. | iron |
| C. | carbon |
| D. | calcium |
| Answer» E. | |
| 8. |
In the secondary culture, cells are obtained from _______________________ |
| A. | primary culture |
| B. | the organism |
| C. | organ culture |
| D. | phenotypic culture |
| Answer» B. the organism | |
| 9. |
Which of the following is NOT the part of growth medium for animal culture? |
| A. | Starch |
| B. | Serum |
| C. | Carbon source |
| D. | Inorganic salts |
| Answer» B. Serum | |
| 10. |
Which of the following is NOT the major function of the serum? |
| A. | Promotion of tuber and bulb formation |
| B. | Stimulate cell growth |
| C. | Enhance cell attachment |
| D. | Provide transport proteins |
| Answer» B. Stimulate cell growth | |
| 11. |
Trypsin is used for dissociating the tissue into single cells. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 12. |
Name the cell line of the human embryonic lung? |
| A. | HeLa |
| B. | WISH |
| C. | L |
| D. | MRC-5 |
| Answer» E. | |
| 13. |
Name the organism on which first cell line was observed? |
| A. | E.coli |
| B. | Sheep |
| C. | Mouse |
| D. | Drosophila |
| Answer» D. Drosophila | |
| 14. |
Which of the following is the characteristics of a normal cell? |
| A. | Anchorage independent |
| B. | Continuous cell lines |
| C. | Dependent on external growth factor |
| D. | No contact inhibition |
| Answer» B. Continuous cell lines | |
| 15. |
What is a cell line? |
| A. | Multilayer culture |
| B. | Transformed cells |
| C. | Multiple growth of cells |
| D. | Sub culturing of primary culture |
| Answer» E. | |
| 16. |
Name the type of culture which is prepared by inoculating directly from the tissue of an organism to culture media? |
| A. | Primary cell culture |
| B. | Secondary cell culture |
| C. | Cell lines |
| D. | Transformed cell culture |
| Answer» B. Secondary cell culture | |
| 17. |
Cells from kidney tissues cannot survive independently, it requires other surface for attachment and survival. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 18. |
Which of the following is the structural fibre in cell culture system |
| A. | Collagen |
| B. | Elastin |
| C. | Fibronectin |
| D. | Both a and b |
| Answer» E. | |
| 19. |
Which of the following is true regarding animal cell culture technique |
| A. | Lactic acid is source of carbon |
| B. | Cells have high requirement of L-glutamine |
| C. | Cholin is necessary for cell adhesion and cytoskeleton |
| D. | All of the following |
| Answer» E. | |
| 20. |
Use of trypsin to free cells from tissue matrix for cell culture was described by |
| A. | Jolly and Koch |
| B. | Beebe and Ewing |
| C. | Sims and Stillman |
| D. | Gottieb Maberland |
| Answer» D. Gottieb Maberland | |
| 21. |
Cells used in feeder layer |
| A. | Should have ability to divide |
| B. | Have ability to metabolize |
| C. | These properties are obtained by exposing cells to irradiation |
| D. | All of the above |
| Answer» E. | |
| 22. |
Cell culture technique became simpler only after advent of |
| A. | Antibiotics |
| B. | Trypsin |
| C. | Cell culture media |
| D. | All of the following |
| Answer» E. | |
| 23. |
The growth of animal cells in vitro in a suitable culture medium is called___________ |
| A. | Gene expression |
| B. | Transgenesis |
| C. | Plant tissue culture |
| D. | Animal cell culture |
| Answer» E. | |
| 24. |
Excess CO2 suppress cell growth and productivity by |
| A. | inhibiting respiration |
| B. | altering intracellular pH by diffusing across cell membrane |
| C. | both (a) and (b) |
| D. | altering pH of the medium |
| Answer» D. altering pH of the medium | |
| 25. |
Range of osmolarity tolerated/accepted in mOsm/Kg of H2O by mammalian cells is |
| A. | 150-300 |
| B. | 280-360 |
| C. | 300-325 |
| D. | 360-400 |
| Answer» C. 300-325 | |
| 26. |
Which of the followings are the metabolic products of glucose and glutamine? |
| A. | CO2 and NH3 |
| B. | CO2 and lactate |
| C. | Lactate and ammonium |
| D. | Lactate only |
| Answer» D. Lactate only | |
| 27. |
What is the concentration of CO2 required for culturing animal cells? |
| A. | 2-5% |
| B. | 1-10% |
| C. | 10-15% |
| D. | 15-20% |
| Answer» C. 10-15% | |
| 28. |
How does CO2 help in the cell metabolism during cell culture? |
| A. | It participates in the de novo synthesis of purines and pyrimidines |
| B. | Helps in the cells respiration |
| C. | For monitoring pH of the culture |
| D. | All of the above |
| Answer» B. Helps in the cells respiration | |
| 29. |
Specific oxygen consumption rates for mammalian cells are in the range of (where n and prepresents to nano and pico respectively) |
| A. | 0.05-5 nmol of O2/cell/h |
| B. | 5 10 nmol of O2 /cell/h |
| C. | 0.05-5 pmol of O2/cell/h |
| D. | 5 10 pmol of O2/cell/h |
| Answer» D. 5 10 pmol of O2/cell/h | |
| 30. |
Which of the following is HGPRT+ and survives in HAT medium |
| A. | B cells |
| B. | Myeloma cells |
| C. | Hybrid cells |
| D. | Both a and c |
| Answer» E. | |
| 31. |
HAT medium used for the selection of fused hybrid cells in hybridoma culture was introduced by |
| A. | Littlefield and Miller |
| B. | Kohler and Milstein |
| C. | Frish and Jentoft |
| D. | Eagle and Karl |
| Answer» B. Kohler and Milstein | |
| 32. |
The limited replicative capacity of human cells in culture is called |
| A. | Hayflick effect |
| B. | Brownian effect |
| C. | Contact inhibition |
| D. | None of the above |
| Answer» B. Brownian effect | |
| 33. |
The foundation for the development of cell culture technique was laid by |
| A. | Roux |
| B. | Arnold |
| C. | Ross |
| D. | Harrison |
| Answer» B. Arnold | |
| 34. |
Who is regarded as the father of tissue culture |
| A. | Harrison |
| B. | Arnold |
| C. | Ross |
| D. | Roux |
| Answer» B. Arnold | |
| 35. |
The ratio of CO2 : O2 used in cell culture system should be |
| A. | 1:5 |
| B. | 1:13 |
| C. | 1:19 |
| D. | 1:25 |
| Answer» D. 1:25 | |
| 36. |
Which of the following is most commonly used cell fusing agent |
| A. | PEG |
| B. | NaNO3 |
| C. | Sendai virus |
| D. | Polyvinyl alcohal |
| Answer» B. NaNO3 | |
| 37. |
Which cell line is used for production of recombinant sex hormones |
| A. | BHK cell line |
| B. | Vero cell line |
| C. | Hela cell line |
| D. | CHO cell line |
| Answer» E. | |
| 38. |
Which of the following is the oldest cell line |
| A. | Hela cell line |
| B. | Vero cell line |
| C. | CHO cell line |
| D. | BHK cell line |
| Answer» B. Vero cell line | |
| 39. |
For culture of avian cells the optimum temperature requirement is |
| A. | 37 C |
| B. | 40 C |
| C. | 42 C |
| D. | 35 C |
| Answer» C. 42 C | |
| 40. |
The optimum temperature of any cell or organ is called |
| A. | Critical temperature |
| B. | Threshold temperature |
| C. | Ceiling temperature |
| D. | None of the above |
| Answer» D. None of the above | |
| 41. |
Optimum pH required for the growth of mammalian cells is |
| A. | 5.3-7.0 |
| B. | 6.5-7.0 |
| C. | 7.2-7.4 |
| D. | 8.1-8.9 |
| Answer» D. 8.1-8.9 | |