MCQOPTIONS
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MCQOPTIONS
This section includes 18 Mcqs, each offering curated multiple-choice questions to sharpen your Gene Manipulation knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
How many types of deoxynucleoside triphosphates are used in Sanger sequencing? |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» E. | |
| 2. |
____________ is a chemically synthesized oligonucleotide. |
| A. | Klenow fragment |
| B. | DNA |
| C. | Primer |
| D. | RNA |
| Answer» D. RNA | |
| 3. |
The Klenow fragment is basically a _______________ |
| A. | DNA hybrid |
| B. | DNA polymerase |
| C. | RNA polymerase |
| D. | Promoter |
| Answer» C. RNA polymerase | |
| 4. |
THE_KLENOW_FRAGMENT_IS_BASICALLY_A________________?$ |
| A. | DNA hybrid |
| B. | DNA polymerase |
| C. | RNA polymerase |
| D. | Promoter |
| Answer» C. RNA polymerase | |
| 5. |
How many types of deoxynucleoside triphosphates are used in Sanger sequencing?$ |
| A. | 1 |
| B. | 2 |
| C. | 3 |
| D. | 4 |
| Answer» E. | |
| 6. |
____________ is a chemically synthesized oligonucleotide.$ |
| A. | Klenow fragment |
| B. | DNA |
| C. | Primer |
| D. | RNA |
| Answer» D. RNA | |
| 7. |
The function of urea in the sequencing gels is to promote adherence. |
| A. | True |
| B. | False |
| Answer» C. | |
| 8. |
What is the molarity of urea used in sequencing gels? |
| A. | 1 M |
| B. | 3 M |
| C. | 5 M |
| D. | 7 M |
| Answer» E. | |
| 9. |
A sequencing gel is a ________________ gel. |
| A. | Toxic |
| B. | Highly-polymerized |
| C. | High resolution |
| D. | Low resolution |
| Answer» D. Low resolution | |
| 10. |
Prior to getting electrophoresed in the sequencing gel, DNA is ____________ |
| A. | Purified |
| B. | Denatured |
| C. | Synthesized |
| D. | Fragmented |
| Answer» C. Synthesized | |
| 11. |
Which of the following act as chain terminator? |
| A. | Exogenous |
| B. | DNA |
| C. | Deoxynucleotides |
| D. | Dideoxynucleotides |
| Answer» E. | |
| 12. |
Which of the following is used by DNA polymerase as a substrate? |
| A. | Sucrose |
| B. | Lactose |
| C. | Nucleotide |
| D. | Nucleoside |
| Answer» D. Nucleoside | |
| 13. |
What is the main enzyme component of Sanger sequencing? |
| A. | Helicase |
| B. | Polymerase |
| C. | Nuclease |
| D. | Gyrase |
| Answer» C. Nuclease | |
| 14. |
Sequence of which of the following cannot be determined using the Maxam Gilbert method? |
| A. | Bacteria |
| B. | Plants |
| C. | Bacteriophage T7 |
| D. | Plasmid |
| Answer» D. Plasmid | |
| 15. |
Which type of DNA cleavage is done in the Maxam Gilbert method? |
| A. | Edge |
| B. | Interstitial |
| C. | Base-specific |
| D. | Gene-specific |
| Answer» D. Gene-specific | |
| 16. |
Plus and minus sequencing is the other name for Sanger sequencing. |
| A. | True |
| B. | False |
| Answer» B. False | |
| 17. |
The first significant DNA sequence to be obtained was that of ________ |
| A. | Lambda |
| B. | Plasmid |
| C. | Lactose |
| D. | Mammals |
| Answer» B. Plasmid | |
| 18. |
Chain-termination is a type of ______________ |
| A. | Sequencing |
| B. | Vector generation |
| C. | Antibiotic production |
| D. | Gene manipulation |
| Answer» B. Vector generation | |