Explore topic-wise MCQs in Molecular Biology.

This section includes 13 Mcqs, each offering curated multiple-choice questions to sharpen your Molecular Biology knowledge and support exam preparation. Choose a topic below to get started.

1.

The gaps formed by the hydrolyzing the RNA from RNA:DNA hybrid is filled by DNA polymerase I and the nicks are ligated by T4 ligase.

A. True
B. False
Answer» C.
2.

Which of the following is not used for degrading RNA from RNA:DNA hybrid in replication?

A. RNase A
B. RNase H
C. Polymerase I
D. Exonuclease
Answer» B. RNase H
3.

Primer synthesis short stretches of DNA for replication.

A. True
B. False
Answer» C.
4.

WHICH_OF_THE_FOLLOWING_IS_NOT_USED_FOR_DEGRADING_RNA_FROM_RNA:DNA_HYBRID_IN_REPLICATION??$

A. RNase A
B. RNase H
C. Polymerase I
D. Exonuclease
Answer» B. RNase H
5.

The_gaps_formed_by_the_hydrolyzing_the_RNA_from_RNA:DNA_hybrid_is_filled_by_DNA_polymerase_I_and_the_nicks_are_ligated_by_T4_ligase.$

A. True
B. False
Answer» C.
6.

Primer synthesis short stretches of DNA for replication?

A. True
B. False
Answer» C.
7.

In the case of a circular DNA synthesis how many replication forks are observed?

A. 1
B. 2
C. 3
D. 4
Answer» C. 3
8.

Who was the first person to analyse the process of replication and on which organism?

A. Arthur Kornberg: E. coli
B. John Cairns: E. coli
C. Arthur Kornberg: Bacillus subtilis
D. John Cairns: Bacillus subtilis
Answer» C. Arthur Kornberg: Bacillus subtilis
9.

Which of the following does not affect DNA replication?

A. Antiparallel nature of DNA
B. End specificity of polymerase
C. SSB protein
D. Helicase
Answer» D. Helicase
10.

Replication fork is the junction between the two ___________

A. Unreplicated DNA
B. Newly synthesized DNA
C. Newly separated DNA strands and newly synthesized DNA strands
D. Newly separated DNA strands and the unreplicated DNA
Answer» E.
11.

Which of the following is correctly matched with its subsequent role?

A. Topoisomerase II- can remove both positive and negative supercoil in the DNA duplex
B. Polymerase I- larger fragment responsible for exonuclease activity
C. DnaA protein- responsible for “melting” of the DNA double helix during replication
D. DnaB protein- attaches to the newly unwounded single strand of DNA to prevent folding of the strand
Answer» D. DnaB protein- attaches to the newly unwounded single strand of DNA to prevent folding of the strand
12.

Pick the correct pair with respect to primers used in DNA replication.

A. RNA primer- for prokaryotes only
B. DNA primer-for eukaryotes only
C. DNA primer- for both prokaryotes and eukaryotes
D. RNA primer- for both prokaryotes and eukaryotes
Answer» E.
13.

Semiconservative nature of replication of eukaryotic chromosome was first demonstrated by _______________

A. Walter Flemming on root tip cells of Vicia faba
B. J. Herbert Taylor, Philip Wood and Walter Hughes on root tip cells of Vicia faba
C. Walter Flemming on root tip cells of Phaseolus vulgaris
D. J. Herbert Taylor, Philip Wood and Walter Hughes on root tip cells of Phaseolus vulgaris
Answer» C. Walter Flemming on root tip cells of Phaseolus vulgaris