Explore topic-wise MCQs in Biochemistry.

This section includes 7 Mcqs, each offering curated multiple-choice questions to sharpen your Biochemistry knowledge and support exam preparation. Choose a topic below to get started.

1.

Which enzyme used to join bits of DNA?

A. DNA polymerase
B. DNA ligase
C. Endonuclease
D. Primase
Answer» C. Endonuclease
Discussion
2.

Which of the following enzymes is the principal replication enzyme in E. coli?

A. DNA polymerase I
B. DNA polymerase II
C. DNA polymerase III
D. None of the mentioned
Answer» D. None of the mentioned
Discussion
3.

DNA unwinding is done by ____________

A. Ligase
B. Helicase
C. Topoisomerase
D. Hexonuclease
Answer» C. Topoisomerase
Discussion
4.

What is the reaction in DNA replication catalyzed by DNA ligase?

A. Addition of new nucleotides to the leading strand
B. Addition of new nucleotide to the lagging strand
C. Formation of a phosphodiester bond between the 3 -OH of one Okazaki fragment and the 5 -phosphate of the next on the lagging strand
D. Base pairing of the template and the newly formed DNA strand
Answer» D. Base pairing of the template and the newly formed DNA strand
Discussion
5.

Eukaryotes differ from prokaryote in mechanism of DNA replication due to ____________

A. Use of DNA primer rather than RNA primer
B. Different enzyme for synthesis of lagging and leading strand
C. Discontinuous rather than semi-discontinuous replication
D. Unidirectional rather than semi-discontinuous replication
Answer» D. Unidirectional rather than semi-discontinuous replication
Discussion
6.

Semi-conservative DNA replication was first demonstrated in ____________

A. Drosophila melanogaster
B. Escherichia coli
C. Streptococcus pneumonae
D. Drosophila melanogaster
Answer» B. Escherichia coli
Discussion
7.

What is DNA replication?

A. Conservative
B. Non-conservative
C. Semi-conservative
D. None of the mentioned
Answer» D. None of the mentioned
Discussion