 
			 
			MCQOPTIONS
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				This section includes 16 Mcqs, each offering curated multiple-choice questions to sharpen your Biochemistry knowledge and support exam preparation. Choose a topic below to get started.
| 1. | Binding of the prokaryotic DNA dependent RNA polymerase to promoter site is inhibited by ____________ | 
| A. | Rifampicin | 
| B. | Tetracycline | 
| C. | Puromycin | 
| D. | Streptomycin | 
| Answer» B. Tetracycline | |
| 2. | RNA primer is removed from the Okazaki fragment by ____________ | 
| A. | DNA polymerase I | 
| B. | DNA polymerase II | 
| C. | DNA polymerase III | 
| D. | RNA polymerase | 
| Answer» B. DNA polymerase II | |
| 3. | Repressors bind to ____________ | 
| A. | Promoter | 
| B. | Enhancer | 
| C. | Operator | 
| D. | Hormone response element | 
| Answer» D. Hormone response element | |
| 4. | Actinomycin D is an inhibitor of ____________ | 
| A. | Transcription | 
| B. | Translation | 
| C. | Replication | 
| D. | None of the mentioned | 
| Answer» B. Translation | |
| 5. | What is the role of sigma factor in bacterial RNA polymerase? | 
| A. | Catalyzing RNA synthesis | 
| B. | Positioning RNA polymerase correctly on the DNA template | 
| C. | Terminating RNA synthesis | 
| D. | Unwinding DNA template | 
| Answer» C. Terminating RNA synthesis | |
| 6. | Transcription is catalyzed by ____________ | 
| A. | DNA-dependent RNA polymerases | 
| B. | RNA-dependent DNA polymerases | 
| C. | Reverse transcriptases | 
| D. | DNA ligases | 
| Answer» B. RNA-dependent DNA polymerases | |
| 7. | RNA_PRIMER_IS_REMOVED_FROM_THE_OKAZAKI_FRAGMENT_BY?$ | 
| A. | DNA polymerase I | 
| B. | DNA polymerase II | 
| C. | DNA polymerase III | 
| D. | RNA polymerase | 
| Answer» B. DNA polymerase II | |
| 8. | Binding_of_the_prokaryotic_DNA_dependent_RNA_polymerase_to_promoter_site_is_inhibited_by$ | 
| A. | Rifampicin | 
| B. | Tetracycline | 
| C. | Puromycin | 
| D. | Streptomycin | 
| Answer» B. Tetracycline | |
| 9. | Repressors bind t? | 
| A. | Promoter | 
| B. | Enhancer | 
| C. | Operator | 
| D. | Hormone response element | 
| Answer» D. Hormone response element | |
| 10. | Number of hydrogen bonds that form between U and A in a Watson-Crick base pair interactions? | 
| A. | 0 | 
| B. | 1 | 
| C. | 2 | 
| D. | 3 | 
| Answer» D. 3 | |
| 11. | Actinomycin D is an inhibitor of | 
| A. | Transcription | 
| B. | Translation | 
| C. | Replication | 
| D. | None | 
| Answer» B. Translation | |
| 12. | TBP stands for? | 
| A. | TATA box polymerase | 
| B. | TATA-box binding protein | 
| C. | Transcription associated factor | 
| D. | Transcription factor binding protein | 
| Answer» C. Transcription associated factor | |
| 13. | Role of sigma factor in bacterial RNA polymerase is | 
| A. | Catalyzing RNA synthesis | 
| B. | Positioning RNA polymerase correctly on the DNA template | 
| C. | Terminating RNA synthesis | 
| D. | Unwinding DNA template | 
| Answer» C. Terminating RNA synthesis | |
| 14. | Which of the following is true about RNA synthesis? | 
| A. | Synthesis of RNA is always in the 5’ to 3’ direction | 
| B. | RNA polymerase requires a primer for initiating transcription | 
| C. | U is inserted opposite T in transcription | 
| D. | New nucleotides are added on the 2’-OH of the ribose sugar | 
| Answer» B. RNA polymerase requires a primer for initiating transcription | |
| 15. | Where does RNA polymerase bind DNA? | 
| A. | Promoter | 
| B. | Operator | 
| C. | Enhancer | 
| D. | None | 
| Answer» B. Operator | |
| 16. | Transcription is catalyzed by | 
| A. | DNA-dependent RNA polymerases | 
| B. | RNA-dependent DNA polymerases | 
| C. | Reverse transcriptases | 
| D. | DNA ligases | 
| Answer» B. RNA-dependent DNA polymerases | |