Explore topic-wise MCQs in Biotechnology.

This section includes 29 Mcqs, each offering curated multiple-choice questions to sharpen your Biotechnology knowledge and support exam preparation. Choose a topic below to get started.

1.

Cryptic plasmids
A. do not exhibit any phenotypic trait
B. exhibit many phenotypic traits
C. exhibit one phenotypic traits
D. exhibit antibiotic resistance
Answer» B. exhibit many phenotypic traits
Discussion
2.

Phagemid consist of
A. plasmid vector carrying phage's cos site
B. plasmid vector carrying attachment ( att) site
C. plasmid vector carrying origin of replication of phage only
D. plasmid vector carrying origin of replication of plasmid only
Answer» C. plasmid vector carrying origin of replication of phage only
Discussion
3.

Maximum size of foreign DNA that can be inserted into an insertion vector is
A. 35 kb
B. 18 kb
C. 50 kb
D. 27 kb
Answer» C. 50 kb
Discussion
4.

Plasmids which are maintained as multiple copy number per cell are known as
A. stringent plasmids
B. relaxed plasmids
C. cryptic plasmids
D. none of these
Answer» C. cryptic plasmids
Discussion
5.

EMBL 3 and EMBL 4 are replacement vectors, which can clone DNA up to
A. 6-7 kb
B. 15-25 kb
C. 40-44 kb
D. 1-2 kb
Answer» C. 40-44 kb
Discussion
6.

Size of the DNA that can be packaged into a phage is
A. 50 kb
B. 35-53 kb
C. 40-50 kb
D. any size
Answer» C. 40-50 kb
Discussion
7.

Cosmid vectors are used for
A. cloning small fragments of DNA
B. cloning large fragments of DNA
C. cloning prokaryotic DNA only
D. cloning eukaryotic DNA only
Answer» C. cloning prokaryotic DNA only
Discussion
8.

Plasmids which are maintained as limited number of copies per cell are known as
A. stringent plasmids
B. relaxed plasmids
C. cryptic plasmids
D. all of these
Answer» B. relaxed plasmids
Discussion
9.

M 13 is an example of
A. filamentous phage
B. single stranded DNA vector
C. both (a) and (b)
D. plasmid
Answer» D. plasmid
Discussion
10.

Phagemid vectors are
A. combination of plasmid and phage
B. combination of phages and cosmid
C. phages carrying properties of plasmids
D. all of the above
Answer» B. combination of phages and cosmid
Discussion
11.

Cosmid vectors are
A. plasmids that contain fragment of DNA including the cos site
B. phages that lack cos site
C. plasmids that have no selection marker
D. cryptic plasmids
Answer» B. phages that lack cos site
Discussion
12.

P1 cloning vector is the example of
A. plasmid
B. cosmid
C. bacteriophage
D. phagemid
Answer» D. phagemid
Discussion
13.

ZAP vector is an example of
A. phage
B. phagemid
C. cosmid
D. plasmid
Answer» C. cosmid
Discussion
14.

Stuffer is
A. the right arm of the vector DNA
B. the left arm of the vector DNA
C. central fragment of the vector DNA
D. none of the above
Answer» D. none of the above
Discussion
15.

Cosmids lack
A. genes coding for viral proteins
B. origin of replication
C. marker genes coding for replication
D. cleavage site for the insertion of foreign DNA
Answer» B. origin of replication
Discussion
16.

Maximum size of foreign DNA that can be inserted into a replacement vector is
A. 25-30 kb
B. 18-20 kb
C. 20-25 kb
D. 40-50 kb
Answer» D. 40-50 kb
Discussion
17.

A plasmid can be considered as a suitable cloning vector if
A. it can be readily isolated from the cells
B. it possesses a single restriction site for one or more restriction enzymes
C. insertion of foreign DNA does not alter its replication properties
D. All of the above
Answer» E.
Discussion
18.

Inserted DNA in gt 11 can be expressed as
A. -galactosidase fused protein
B. free protein in the cytoplasm
C. free protein that is secreted out
D. all of the above
Answer» B. free protein in the cytoplasm
Discussion
19.

P1 cloning vector allow cloning of DNA of the length of
A. 100 kbp
B. 50 kbp
C. 20 kbp
D. 10 kbp
Answer» B. 50 kbp
Discussion
20.

Difference between gt 10 and gt 11 vectors is that
A. gt 11 is an expression vector
B. gt 10 is an expression vector
C. gt 10 is a replacement vector
D. gt 11 is a replacement vector
Answer» B. gt 10 is an expression vector
Discussion
21.

Charon 34 and Charon 35 are the examples of
A. plasmid vector
B. cosmid vector
C. phage vector
D. phagemid vector
Answer» D. phagemid vector
Discussion
22.

Which of the following is not true about phagemid?
A. Contain functional origin of replication of the plasmid and phage
B. May be propagated as a plasmid or as phage in appropriate strain
C. Contain att site
D. Can only be propagated as phage
Answer» E.
Discussion
23.

Plasmid incompatibility is
A. inability of a plasmid to grow in the host
B. inability of two different plasmids to coexist in the same host cell in the absence of selection pressure.
C. both (a) and (b)
D. none of the above
Answer» C. both (a) and (b)
Discussion
24.

gt 10 and gt 11 vectors can propagate cloned fragments up to
A. 6-7 kb
B. 1-2 kb
C. 40-44 kb
D. 20-23 kb
Answer» B. 1-2 kb
Discussion
25.

Single stranded vectors are useful
A. for sequencing of cloned DNA
B. for oligonucleotide directed mutagenesis
C. for probe preparation
D. all of the above
Answer» E.
Discussion
26.

Cos site of the cosmids
A. consists of 12 bases
B. helps whole genome in circularization and ligation
C. both (a) and (b)
D. contains cleavage site
Answer» D. contains cleavage site
Discussion
27.

Select the wrong statement about plasmids?
A. It is extrachromosomal
B. It is double stranded
C. Its replication depends upon host cell
D. It is closed and circular DNA
Answer» D. It is closed and circular DNA
Discussion
28.

Charon 34 and Charon 35 can clone DNA upto
A. 1-2 kb
B. 6-7 kb
C. 9-20 kb
D. 30-35 kb
Answer» D. 30-35 kb
Discussion
29.

Charon vectors are different from EMBL vectors because
A. they have more extensive range of restriction targets with in their polylinkers.
B. physical separation of lambda arm from central fragment is required
C. both (a) and (b)
D. physical separation of lambda arm from central fragment is not required
Answer» D. physical separation of lambda arm from central fragment is not required
Discussion