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				This section includes 46 Mcqs, each offering curated multiple-choice questions to sharpen your Graduate Aptitude Test (GATE) knowledge and support exam preparation. Choose a topic below to get started.
| 1. | 
                                    What is the relationship between ∆G and ∆G‘°? | 
                            
| A. | ∆G = ∆G‘° + RTln([products]/[reactants]) | 
| B. | ∆G = ∆G‘° – RTln([products]/[reactants]) | 
| C. | ∆G = ∆G‘° + RTln([reactants]/[products]) | 
| D. | ∆G = ∆G‘° – RTln([reactants]/[products]) | 
| Answer» B. ∆G = ∆G‘° – RTln([products]/[reactants]) | |
| 2. | 
                                    If ∆G‘° of the reaction A → B is -40kJ/mol under standard conditions then the reaction | 
                            
| A. | Will never reach equilibrium | 
| B. | Will not occur spontaneously | 
| C. | Will proceed at a rapid rate | 
| D. | Will proceed from left to right spontaneously | 
| Answer» E. | |
| 3. | 
                                     The study of energy relationships and conversions in biological systems is called as | 
                            
| A. | Biophysics | 
| B. | Biotechnology | 
| C. | Bioenergetics | 
| D. | Microbiology | 
| Answer» D. Microbiology | |
| 4. | 
                                     Unfolding of regular secondary protein structure causes | 
                            
| A. | Large decrease in the entropy of the protein | 
| B. | Little increase in the entropy of protein | 
| C. | No change in the entropy of the protein | 
| D. | Large increase in the entropy of the protein | 
| Answer» E. | |
| 5. | 
                                     For a reaction if ∆G° is positive, then | 
                            
| A. | The products will be favored | 
| B. | The reactants will be favored | 
| C. | The concentration of the reactants and products will be equal | 
| D. | All of the reactant will be converted to products | 
| Answer» C. The concentration of the reactants and products will be equal | |
| 6. | 
                                     ∆G° is defined as the | 
                            
| A. | Residual energy present in the reactants at equilibrium | 
| B. | Residual energy present in the products at equilibrium | 
| C. | Difference in the residual energy of reactants and products at equilibrium | 
| D. | Energy required in converting one mole of reactants to one mole of products | 
| Answer» E. | |
| 7. | 
                                     Glycolysis converts | 
                            
| A. | Glucose into pyruvate | 
| B. | Glucose into phosphoenolpyruvate | 
| C. | Fructose into pyruvate | 
| D. | Fructose into phosphoenolpyruvate | 
| Answer» B. Glucose into phosphoenolpyruvate | |
| 8. | 
                                    The product formed in the first substrate level phosphorylation in glycolysis is | 
                            
| A. | Pyruvate | 
| B. | 3-phosphoglycerate | 
| C. | 1, 3-bisphosphoglycerate | 
| D. | 2-phosphoglycerate | 
| Answer» C. 1, 3-bisphosphoglycerate | |
| 9. | 
                                    High concentration of glucose 6-phosphate is inhibitory to | 
                            
| A. | Hexokinase | 
| B. | Pyruvate kinase | 
| C. | Glucokinase | 
| D. | Phosphofructokinase-1 | 
| Answer» B. Pyruvate kinase | |
| 10. | 
                                     The substrate used in the last step of glycolysis is | 
                            
| A. | Glyceraldehyde 3-phosphate | 
| B. | Pyruvate | 
| C. | Phosphoenolpyruvate | 
| D. | 1, 3-bisphosphoglycerate | 
| Answer» D. 1, 3-bisphosphoglycerate | |
| 11. | 
                                     The first step in the payoff phase of glycolysis is | 
                            
| A. | Reduction of 1, 3-bisphosphoglycerate to glyceraldehyde 3-phosphate | 
| B. | Oxidation of glyceraldehyde 3-phosphate to 1, 3-bisphosphoglycerate | 
| C. | Reversible conversion of dihydroxyacetone phosphate to glyceraldehyde 3-phosphate | 
| D. | Irreversible conversion of dihydroxyacetone phosphate to glyceraldehyde 3-phosphate | 
| Answer» C. Reversible conversion of dihydroxyacetone phosphate to glyceraldehyde 3-phosphate | |
| 12. | 
                                    Dihydroxyacetone phosphate is rapidly and reversibly converted to | 
                            
| A. | Glyceraldehyde 3-phosphate | 
| B. | 1, 3-bis-phosphoglycerate | 
| C. | Fructose 1, 6-bisphosphate | 
| D. | Fructose 6-phosphate | 
| Answer» B. 1, 3-bis-phosphoglycerate | |
| 13. | 
                                     Cleavage of Fructose 1, 6-biophosphate yields | 
                            
| A. | Two aldoses | 
| B. | Two ketoses | 
| C. | An aldose and a ketose | 
| D. | Only a ketose | 
| Answer» D. Only a ketose | |
| 14. | 
                                     Whenever the cell’s ATP supply is depleted, which of the following enzyme’s activity is increased? | 
                            
| A. | Hexokinase | 
| B. | Pyruvate kinase | 
| C. | Glucokinase | 
| D. | Phosphofructokinase-1 | 
| Answer» E. | |
| 15. | 
                                    The general term used for the anaerobic degradation of glucose to obtain energy is | 
                            
| A. | Anabolism | 
| B. | Oxidation | 
| C. | Oxidation | 
| D. | Metabolism | 
| Answer» D. Metabolism | |
| 16. | 
                                    Which of the following enzyme catalyzes the first step of glycolysis? | 
                            
| A. | Hexokinase | 
| B. | Pyruvate kinase | 
| C. | Glucokinase | 
| D. | Phosphofructokinase-1 | 
| Answer» B. Pyruvate kinase | |
| 17. | 
                                     Which out of the following enzymes can utilize both NAD+ and NADP+ as a coenzyme? | 
                            
| A. | Glutamate dehydrogenase | 
| B. | Alcohol dehydrogenase | 
| C. | Aldehyde dehydrogenase | 
| D. | Glycerol-3-P ddehydrogenase | 
| Answer» B. Alcohol dehydrogenase | |
| 18. | 
                                    Which of the following enzyme catalyzes the first step in the catabolism of ethanol? | 
                            
| A. | Alcohol dehydrogenase | 
| B. | Lactate dehydrogenase | 
| C. | Glutamate dehydrogenase | 
| D. | Malate dehydrogenase | 
| Answer» B. Lactate dehydrogenase | |
| 19. | 
                                    Gain of electrons can be termed as | 
                            
| A. | Metabolism | 
| B. | Anabolism | 
| C. | Oxidation | 
| D. | Reduction | 
| Answer» E. | |
| 20. | 
                                    Loss of electrons can be termed as | 
                            
| A. | Metabolism | 
| B. | Anabolism | 
| C. | Oxidation | 
| D. | Reduction | 
| Answer» D. Reduction | |
| 21. | 
                                    Which of the following statements is incorrect about oxidase? | 
                            
| A. | They catalyze reactions involving hydrogen peroxide | 
| B. | They catalyze reaction using O2 as a hydrogen acceptor | 
| C. | They catalyze reactions using niacin as coenzyme | 
| D. | They catalyze reactions of direct incorporation of O2 into the substrate | 
| Answer» C. They catalyze reactions using niacin as coenzyme | |
| 22. | 
                                    Which of the following enzyme catalyses the direct transfer and incorporation of O2 into a substrate molecule is | 
                            
| A. | Reductase | 
| B. | Oxidase | 
| C. | Oxygenase | 
| D. | Peroxidase | 
| Answer» D. Peroxidase | |
| 23. | 
                                    Which one out of the following is not a NAD+ requiring enzyme? | 
                            
| A. | Lactate dehydrogenase | 
| B. | Pyruvate dehydrogenase complex | 
| C. | Maltate dehydrogenase | 
| D. | Acyl co-A dehydrogenase | 
| Answer» E. | |
| 24. | 
                                    Which out of the following has the highest redox potential? | 
                            
| A. | NAD | 
| B. | FMN | 
| C. | FAD | 
| D. | O2 | 
| Answer» E. | |
| 25. | 
                                     Which out of the following is not a flavoprotein? | 
                            
| A. | Succinate dehydrogenase | 
| B. | Cytochrome c | 
| C. | Xanthine oxidase | 
| D. | NADH dehydrogenase-Qreductase | 
| Answer» C. Xanthine oxidase | |
| 26. | 
                                     Which of the following statements about redox potential is false? | 
                            
| A. | NADH/NAD+ redox pair has the least redox potential | 
| B. | Oxygen/H2O redox pair has the highest redox potential | 
| C. | The components of electron transport chain are organized in terms of their redox potential | 
| D. | The redox potential of a system is usually compared with the potential of the hydrogen electrode | 
| Answer» B. Oxygen/H2O redox pair has the highest redox potential | |
| 27. | 
                                    An example for chemical to electromagnetic energy conversion that occurs in living organisms | 
                            
| A. | ATP-driven muscle contraction | 
| B. | ATP-dependent photon emission in fireflies | 
| C. | light-induced electron flow in chloroplasts | 
| D. | ATP-driven active transport across a membrane | 
| Answer» C. light-induced electron flow in chloroplasts | |
| 28. | 
                                    An example for chemical to osmotic energy conversion that occurs in living organisms | 
                            
| A. | ATP-driven muscle contraction | 
| B. | ATP-dependent photon emission in fireflies | 
| C. | light-induced electron flow in chloroplasts | 
| D. | ATP-driven active transport across a membrane | 
| Answer» E. | |
| 29. | 
                                    Which of the following is false about acetyl-coA? | 
                            
| A. | It is a thioester | 
| B. | It has a large, negative standard free energy of hydrolysis | 
| C. | It contains phosphorous atom in the position occupied by an oxygen atom in oxygen esters | 
| D. | It contains sulfur atom in the position occupied by an oxygen tom in oxygen esters | 
| Answer» D. It contains sulfur atom in the position occupied by an oxygen tom in oxygen esters | |
| 30. | 
                                    The phosphate groups used during glycolysis are | 
                            
| A. | Organic | 
| B. | Inorganic | 
| C. | Neutral | 
| D. | None | 
| Answer» C. Neutral | |
| 31. | 
                                     Water does a nucleophilic attack on phosphate monoester producing | 
                            
| A. | Phosphorous chloride | 
| B. | Phosphorous sulfide | 
| C. | Inorganic phosphate | 
| D. | Organic phosphate | 
| Answer» D. Organic phosphate | |
| 32. | 
                                     Phosphoryl groups are derivatives of | 
                            
| A. | Phosphorous acid | 
| B. | Phosphoric acid | 
| C. | Acetic acid | 
| D. | Citric acid | 
| Answer» C. Acetic acid | |
| 33. | 
                                    An exergonic reaction | 
                            
| A. | Proceeds spontaneously | 
| B. | Does not require activation energy | 
| C. | Releases energy | 
| D. | Requires energy | 
| Answer» D. Requires energy | |
| 34. | 
                                    An endergonic reaction | 
                            
| A. | Proceeds spontaneously | 
| B. | Does not require activation energy | 
| C. | Releases energy | 
| D. | Requires energy | 
| Answer» E. | |
| 35. | 
                                    Which of the following statements is false about ATP hydrolysis? | 
                            
| A. | It is highly exergonic | 
| B. | Activation energy is relatively high | 
| C. | ∆G‘°=-30.5kJ/mol | 
| D. | ∆G‘°=30.5kJ/mol | 
| Answer» E. | |
| 36. | 
                                     Anabolism and catabolism are chemically linked in the form of | 
                            
| A. | ADP | 
| B. | ATP | 
| C. | Phosphodiester linkage | 
| D. | ASP | 
| Answer» C. Phosphodiester linkage | |
| 37. | 
                                    Which of the following statements is false? | 
                            
| A. | The reaction tends to go in the forward direction if ∆G is large and positive | 
| B. | The reaction tends to move in the backward direction if ∆G is large and negative | 
| C. | The system is at equilibrium if ∆G = 0 | 
| D. | The reaction tends to move in the backward direction if ∆G is large and positive | 
| Answer» E. | |
| 38. | 
                                    What is the relationship between ∆G and ∆G‘°? | 
                            
| A. | ∆G = ∆G‘° + RTln([products]/[reactants]) | 
| B. | ∆G = ∆G‘° – RTln([products]/[reactants]) | 
| C. | ∆G = ∆G‘° + RTln([reactants]/[products]) | 
| D. | ∆G = ∆G‘° – RTln([reactants]/[products]) | 
| Answer» B. ∆G = ∆G‘° – RTln([products]/[reactants]) | |
| 39. | 
                                     If ∆G‘° of the reaction A → B is -40kJ/mol under standard conditions then the reaction | 
                            
| A. | Will never reach equilibrium | 
| B. | Will not occur spontaneously | 
| C. | Will proceed at a rapid rate | 
| D. | Will proceed from left to right spontaneously | 
| Answer» E. | |
| 40. | 
                                    What does first law of thermodynamics state? | 
                            
| A. | Energy can neither be destroyed nor created | 
| B. | Energy cannot be 100 percent efficiently transformed from one type to another | 
| C. | All living organisms are composed of cells | 
| D. | Input of heat energy increases the rate of movement of atoms and molecules | 
| Answer» B. Energy cannot be 100 percent efficiently transformed from one type to another | |
| 41. | 
                                    The relationship between K‘eq and ∆G‘° is | 
                            
| A. | ∆G‘° = RTlnK‘eq | 
| B. | ∆G‘° = -RTK‘eq | 
| C. | ∆G‘° = RK‘eq | 
| D. | ∆G‘° = -RK‘eq | 
| Answer» C. ∆G‘° = RK‘eq | |
| 42. | 
                                    The study of energy relationships and conversions in biological systems is called as | 
                            
| A. | Biophysics | 
| B. | Biotechnology | 
| C. | Bioenergetics | 
| D. | Microbiology | 
| Answer» D. Microbiology | |
| 43. | 
                                    Unfolding of regular secondary protein structure causes | 
                            
| A. | Large decrease in the entropy of the protein | 
| B. | Little increase in the entropy of protein | 
| C. | No change in the entropy of the protein | 
| D. | Large increase in the entropy of the protein | 
| Answer» E. | |
| 44. | 
                                    For a reaction if ∆G° is positive, then | 
                            
| A. | The products will be favored | 
| B. | The reactants will be favored | 
| C. | The concentration of the reactants and products will be equal | 
| D. | All of the reactant will be converted to products | 
| Answer» C. The concentration of the reactants and products will be equal | |
| 45. | 
                                    ∆G° is defined as the | 
                            
| A. | Residual energy present in the reactants at equilibrium | 
| B. | Residual energy present in the products at equilibrium | 
| C. | Difference in the residual energy of reactants and products at equilibrium | 
| D. | Energy required in converting one mole of reactants to one mole of products | 
| Answer» E. | |
| 46. | 
                                     If enthalpy change for a reaction is zero, then ∆G° equals to | 
                            
| A. | -T∆S° | 
| B. | T∆S° | 
| C. | -∆H° | 
| D. | lnkeq | 
| Answer» B. T∆S° | |