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This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Gene Manipulation knowledge and support exam preparation. Choose a topic below to get started.
1. |
What are the lectins? |
A. | tAntigen |
B. | tAntibody |
C. | tDNA |
D. | tRNA |
Answer» C. tDNA | |
2. |
Lectins identify which of the following in a Western blotting technique? |
A. | tGlucoprotein |
B. | tGlycoprotein |
C. | tAntibiotic |
D. | tRNA |
Answer» C. tAntibiotic | |
3. |
Which is the most common ligand in Western blotting? |
A. | tLactose |
B. | tToxins |
C. | tGenes |
D. | tAntibodies |
Answer» E. | |
4. |
Which interactions are made use of in Western blotting technique? |
A. | tCovalent |
B. | tHydrophobic |
C. | tProtein-ligand |
D. | tProtein-protein |
Answer» D. tProtein-protein | |
5. |
In Western blotting, proteins bind __________ to the membrane. |
A. | tLoosely |
B. | tCovalently |
C. | tIrreversibly |
D. | tStrongly |
Answer» E. | |
6. |
The protein bands transferred by the western blotting are previously ______________ |
A. | tElectrophoresed |
B. | tHeated |
C. | tCalibrated |
D. | tMixed |
Answer» B. tHeated | |
7. |
When was the Western blotting technique developed? |
A. | t1980 |
B. | t1961 |
C. | t1981 |
D. | t1971 |
Answer» D. t1971 | |
8. |
Western Blotting is used to transfer __________ |
A. | tGenes |
B. | tProteins |
C. | tDNA |
D. | tProbe |
Answer» C. tDNA | |
9. |
Which of the following techniques does not involve nucleic acids? |
A. | tNorthern blotting |
B. | tWestern blotting |
C. | tPFGE |
D. | tSouthern blotting |
Answer» C. tPFGE | |
10. |
Nylon membranes have superseded the need of ___________ in southern blotting techniques. |
A. | tProbe |
B. | tRNA |
C. | tDBM paper |
D. | tGel |
Answer» D. tGel | |
11. |
When was it found that RNA can be bound to nitrocellulose membranes under appropriate conditions? |
A. | t1980 |
B. | t1880 |
C. | t1940 |
D. | t1950 |
Answer» B. t1880 | |
12. |
How can hybridizing bands be located? |
A. | tRadiography |
B. | tAutoradiography |
C. | tUV radiation |
D. | tInfrared radiation |
Answer» C. tUV radiation | |
13. |
The reactive paper used is prepared by which technique? |
A. | tHeating |
B. | tHydrolysis |
C. | tAcidification |
D. | tDiazotization |
Answer» E. | |
14. |
Which of the following is found in Northern blotting but not in southern blotting? |
A. | tGel |
B. | tNitrocellulose |
C. | tReactive paper |
D. | tProbe |
Answer» D. tProbe | |
15. |
Northern Blotting is not a variant of Southern blotting. |
A. | tTrue |
B. | tFalse |
Answer» C. | |