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This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Gene Manipulation knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
What are the lectins? |
| A. | tAntigen |
| B. | tAntibody |
| C. | tDNA |
| D. | tRNA |
| Answer» C. tDNA | |
| 2. |
Lectins identify which of the following in a Western blotting technique? |
| A. | tGlucoprotein |
| B. | tGlycoprotein |
| C. | tAntibiotic |
| D. | tRNA |
| Answer» C. tAntibiotic | |
| 3. |
Which is the most common ligand in Western blotting? |
| A. | tLactose |
| B. | tToxins |
| C. | tGenes |
| D. | tAntibodies |
| Answer» E. | |
| 4. |
Which interactions are made use of in Western blotting technique? |
| A. | tCovalent |
| B. | tHydrophobic |
| C. | tProtein-ligand |
| D. | tProtein-protein |
| Answer» D. tProtein-protein | |
| 5. |
In Western blotting, proteins bind __________ to the membrane. |
| A. | tLoosely |
| B. | tCovalently |
| C. | tIrreversibly |
| D. | tStrongly |
| Answer» E. | |
| 6. |
The protein bands transferred by the western blotting are previously ______________ |
| A. | tElectrophoresed |
| B. | tHeated |
| C. | tCalibrated |
| D. | tMixed |
| Answer» B. tHeated | |
| 7. |
When was the Western blotting technique developed? |
| A. | t1980 |
| B. | t1961 |
| C. | t1981 |
| D. | t1971 |
| Answer» D. t1971 | |
| 8. |
Western Blotting is used to transfer __________ |
| A. | tGenes |
| B. | tProteins |
| C. | tDNA |
| D. | tProbe |
| Answer» C. tDNA | |
| 9. |
Which of the following techniques does not involve nucleic acids? |
| A. | tNorthern blotting |
| B. | tWestern blotting |
| C. | tPFGE |
| D. | tSouthern blotting |
| Answer» C. tPFGE | |
| 10. |
Nylon membranes have superseded the need of ___________ in southern blotting techniques. |
| A. | tProbe |
| B. | tRNA |
| C. | tDBM paper |
| D. | tGel |
| Answer» D. tGel | |
| 11. |
When was it found that RNA can be bound to nitrocellulose membranes under appropriate conditions? |
| A. | t1980 |
| B. | t1880 |
| C. | t1940 |
| D. | t1950 |
| Answer» B. t1880 | |
| 12. |
How can hybridizing bands be located? |
| A. | tRadiography |
| B. | tAutoradiography |
| C. | tUV radiation |
| D. | tInfrared radiation |
| Answer» C. tUV radiation | |
| 13. |
The reactive paper used is prepared by which technique? |
| A. | tHeating |
| B. | tHydrolysis |
| C. | tAcidification |
| D. | tDiazotization |
| Answer» E. | |
| 14. |
Which of the following is found in Northern blotting but not in southern blotting? |
| A. | tGel |
| B. | tNitrocellulose |
| C. | tReactive paper |
| D. | tProbe |
| Answer» D. tProbe | |
| 15. |
Northern Blotting is not a variant of Southern blotting. |
| A. | tTrue |
| B. | tFalse |
| Answer» C. | |