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This section includes 10 Mcqs, each offering curated multiple-choice questions to sharpen your Molecular Biology knowledge and support exam preparation. Choose a topic below to get started.
1. |
A third generation sequencing can sequence upto ____________nucleotides per day. |
A. | 750000 |
B. | 75000 |
C. | 570000 |
D. | 57000 |
Answer» B. 75000 | |
2. |
No chain termination takes place in automated sequencing. |
A. | True |
B. | False |
Answer» C. | |
3. |
A third generation sequencing can sequence upto ____________nucleotides per day? |
A. | 750000 |
B. | 75000 |
C. | 570000 |
D. | 57000 |
Answer» B. 75000 | |
4. |
Automated sequencing is a real time one trial method of sequencing and is highly handy. |
A. | True |
B. | False |
Answer» C. | |
5. |
Which of the following feature is required for the high sensitivity of the tags used for autosequencing? |
A. | Strong absorption at UV spectrum |
B. | Maximum emittion at different wavelengths |
C. | Different mobility shifts |
D. | Induce circularization of DNA fragments |
Answer» C. Different mobility shifts | |
6. |
With respect to automated sequencing which of the following is true? |
A. | Detected by radioactive tags |
B. | Multiple plane sequencing |
C. | Horizontal scanning one sequence per lane |
D. | Vertical reading software incorporation |
Answer» D. Vertical reading software incorporation | |
7. |
As different fluorescent tags affect the mobility of fragments thus only one type of tag is used. |
A. | True |
B. | False |
Answer» C. | |
8. |
No chain termination takes place in automated sequencing ____________ |
A. | True |
B. | False |
Answer» C. | |
9. |
Automated sequencing uses _____________ |
A. | Enzymatic process |
B. | Radiolabelling |
C. | Chain termination |
D. | Fluorescent tagging |
Answer» E. | |
10. |
Automated sequencing is defined as _________ |
A. | Chain termination sequencing |
B. | Radio labeled sequencing |
C. | Real time fluorescence sequencing |
D. | Pyrosequencing |
Answer» D. Pyrosequencing | |