 
			 
			MCQOPTIONS
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				This section includes 18 Mcqs, each offering curated multiple-choice questions to sharpen your Biochemistry knowledge and support exam preparation. Choose a topic below to get started.
| 1. | Each cycle of β-oxidation produces | 
| A. | 1 FADH2, 1 NAD+, and 1 acetyl-CoA | 
| B. | 1 FADH2, 1 NADH and 1 acetyl-CoA | 
| C. | 1 FADH2, 1 NADH and 2 CO2 molecules | 
| D. | 1 FAD, 1 NAD+ and 2 CO2 molecules | 
| Answer» C. 1 FADH2, 1 NADH and 2 CO2 molecules | |
| 2. | Which of the following accepts only one electron? | 
| A. | Cytochrome b | 
| B. | Coenzyme Q | 
| C. | FMN | 
| D. | FAD | 
| Answer» B. Coenzyme Q | |
| 3. | Which one of the following is the one having highest redox potential? | 
| A. | Ubiquinone | 
| B. | O2 | 
| C. | FMN | 
| D. | NAD | 
| Answer» C. FMN | |
| 4. | Chemiosmotic hypothesis does not involve ____________ | 
| A. | Only proton transport is strictly regulated, other positively charged ions can diffuse freely across the mitochondrial membrane | 
| B. | ATPase activity is reversible | 
| C. | Proton flow into the mitochondria depends on the presence of ADP and Pi | 
| D. | Electron transport by the respiratory chain pumps protons out of the mitochondria | 
| Answer» B. ATPase activity is reversible | |
| 5. | If rotenone is added to the mitochondrial electron transport chain then ____________ | 
| A. | Succinate oxidation remains normal | 
| B. | P: O ratio of NADH is reduced from 3:1 to 2:1 | 
| C. | Oxidative phosphorylation is uncoupled at site I | 
| D. | Rate of NADH oxidation is diminished to two-thirds of its initial value | 
| Answer» B. P: O ratio of NADH is reduced from 3:1 to 2:1 | |
| 6. | If the oxidative phosphorylation was uncoupled in the mitochondria then there is a/an ____________ | 
| A. | Decreased concentration of ADP in the mitochondria | 
| B. | Decreased oxidative rate | 
| C. | Increased inorganic phosphate in the mitochondria | 
| D. | Decreased production of heat | 
| Answer» D. Decreased production of heat | |
| 7. | If 2, 4-dinitrophenol is added to tightly coupled mitochondria that are actively oxidizing succinate ____________ | 
| A. | Electron flow will continue but ATP synthesis will not occur | 
| B. | Electron flow will continue but ATP synthesis will be increased | 
| C. | Electron flow will cease but ATP synthesis will continue | 
| D. | Both electron flow and ATP synthesis will be ceased | 
| Answer» B. Electron flow will continue but ATP synthesis will be increased | |
| 8. | Effect of valinomycin on oxidative phosphorylation does not involve _____________ | 
| A. | pH gradient across the inner mitochondrial membrane decreases | 
| B. | Rate of flow of electrons increases | 
| C. | Rate of oxygen consumption increases | 
| D. | Net yield of ATP decreases | 
| Answer» B. Rate of flow of electrons increases | |
| 9. | OUT_OF_THE_FOLLOWING_THE_ONE_HAVING_HIGHEST_REDOX_POTENTIAL_IS?$ | 
| A. | Ubiquinone | 
| B. | O<sub>2</sub> | 
| C. | FMN | 
| D. | NAD | 
| Answer» C. FMN | |
| 10. | Which_of_the_following_accepts_only_one_electron?$ | 
| A. | Cytochrome b | 
| B. | Coenzyme Q | 
| C. | FMN | 
| D. | FAD | 
| Answer» B. Coenzyme Q | |
| 11. | Chemiosmotic hypothesis does not involv? | 
| A. | Only proton transport is strictly regulated, other positively charged ions can diffuse freely across the mitochondrial membrane | 
| B. | ATPase activity is reversible | 
| C. | Proton flow in to the mitochondria depends on the presence of ADP and Pi | 
| D. | Electron transport by the respiratory chain pumps protons out of the mitochondria | 
| Answer» B. ATPase activity is reversible | |
| 12. | Which of the following takes place in substrate level phosphorylation? | 
| A. | Oxidation of one molecule of substrate is linked to synthesis of more than one ATP molecule | 
| B. | High energy intermediate compounds cannot be isolated | 
| C. | Only mitochondrial reactions participate in ATP formation | 
| D. | Substrate reacts to form a product containing a high energy bond | 
| Answer» E. | |
| 13. | If rotenone is added to the mitochondrial electron transport chain | 
| A. | Succinate oxidation remains normal | 
| B. | P: O ratio of NADH is reduced from 3:1 to 2:1 | 
| C. | Oxidative phosphorylation is uncoupled at site I | 
| D. | Rate of NADH oxidation is diminished to two-thirds of its initial value | 
| Answer» B. P: O ratio of NADH is reduced from 3:1 to 2:1 | |
| 14. | If the oxidative phosphorylation was uncoupled in the mitochondria then there is a/an | 
| A. | Decreased concentration of ADP in the mitochondria | 
| B. | Decreased oxidative rate | 
| C. | Increased inorganic phosphate in the mitochondria | 
| D. | Decreased production of heat | 
| Answer» D. Decreased production of heat | |
| 15. | If 2, 4-dinitrophenol is added to tightly coupled mitochondria that are actively oxidizing succinate | 
| A. | Electron flow will continue but ATP synthesis will not occur | 
| B. | Electron flow will continue but ATP synthesis will be increased | 
| C. | Electron flow will cease but ATP synthesis will continue | 
| D. | Both electron flow and ATP synthesis will be ceased | 
| Answer» B. Electron flow will continue but ATP synthesis will be increased | |
| 16. | If mitochondria were blocked at the site of NADH oxidation and were treated with succinate as substrate, what would the P : O ratio is? | 
| A. | Same as that normally produced by succinate | 
| B. | One more than normally produced by succinate | 
| C. | One less than normally produced by succinate | 
| D. | Zero | 
| Answer» B. One more than normally produced by succinate | |
| 17. | Effect of valinomycin on oxidative phosphorylation does not involve | 
| A. | pH gradient across the inner mitochondrial membrane decreases | 
| B. | Rate of flow of electrons increases | 
| C. | Rate of oxygen consumption increases | 
| D. | Net yield of ATP decreases | 
| Answer» B. Rate of flow of electrons increases | |
| 18. | Which of the following is the prosthetic group of NADH dehydrogenase? | 
| A. | NADH | 
| B. | FAD | 
| C. | NADPH | 
| D. | FMN | 
| Answer» E. | |