MCQOPTIONS
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MCQOPTIONS
This section includes 13 Mcqs, each offering curated multiple-choice questions to sharpen your Protein Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
An ideal affinity tag should display an easily controllable binding behavior with sufficient affinity. |
| A. | False |
| B. | True |
| Answer» C. | |
| 2. |
Strep-tag I can be used at which of the following positions? |
| A. | Carboxy or amino-terminal |
| B. | Amino-terminal |
| C. | In between two protein domains |
| D. | Carboxy terminal |
| Answer» E. | |
| 3. |
Which of the following is used in the column regeneration step of Twin-Strep-tag: Strep-Tactin XT purification, that displaces biotin? |
| A. | Desthiobiotin |
| B. | Buffer |
| C. | HABA |
| D. | NaOH |
| Answer» E. | |
| 4. |
Which of the following shows the highest binding affinity for Strep-Tactin? |
| A. | Biotin |
| B. | Strep-tag I |
| C. | Strep-tag II |
| D. | Twin-strep-tag |
| Answer» E. | |
| 5. |
Which of the following is an engineered streptavidin variant? |
| A. | Biotin |
| B. | Strep-tag II |
| C. | Strep-tag I |
| D. | Strep-Tactin |
| Answer» E. | |
| 6. |
Which of the following is used in the column regeneration step of Strep-tag purification, that displaces desthiobiotin? |
| A. | Buffer |
| B. | Biotin |
| C. | NaOH |
| D. | HABA |
| Answer» E. | |
| 7. |
The affinity of Strep-tag for streptavidin is very high when it is placed at the amino terminus or in between two protein domains. |
| A. | True |
| B. | False |
| Answer» C. | |
| 8. |
An ideal affinity tag should interfere with the folding or bioactivity of a recombinant protein. |
| A. | True |
| B. | False |
| Answer» C. | |
| 9. |
Streptavidin is naturally secreted by which of the following microorganisms? |
| A. | <em>Aspergillus niger</em> |
| B. | <em>Escherichia coli</em> |
| C. | <em>Bacillus anthrax</em> |
| D. | <em>Streptomyces avidinii</em> |
| Answer» E. | |
| 10. |
Strep-tag II is sensitive to cellular proteases. |
| A. | True |
| B. | False |
| Answer» C. | |
| 11. |
Which of the following is a homo-tetramer of 159 amino acids per subunit? |
| A. | Biotin |
| B. | Flag peptide |
| C. | S-tag |
| D. | Streptavidin |
| Answer» E. | |
| 12. |
Strep-tag II hampers protein folding or secretion and it usually interferes with protein function. |
| A. | True |
| B. | False |
| Answer» C. | |
| 13. |
Which of the following is an 8-residue minimal peptide sequence that exhibits intrinsic affinity towards streptavidin and can be fused to recombinant proteins in various combinations? |
| A. | Strep-tag I |
| B. | S-tag |
| C. | Flag peptide |
| D. | Strep-tag II |
| Answer» E. | |