MCQOPTIONS
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MCQOPTIONS
This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Protein Engineering knowledge and support exam preparation. Choose a topic below to get started.
| 1. |
Which of the following affinity tag is a 26 amino acid residue peptide which can be bound to calmodulin? |
| A. | Myc-tag |
| B. | His-tag |
| C. | S-tag |
| D. | Calmodulin-binding peptide (CBP) |
| Answer» E. | |
| 2. |
Most of the interactions between the strep-tag and streptavidin are of non-polar nature. |
| A. | True |
| B. | False |
| Answer» C. | |
| 3. |
Strep-tag affinity chromatography requires high salt concentrations, extreme pH, chelator compounds for washing or elution. |
| A. | True |
| B. | False |
| Answer» C. | |
| 4. |
Which of the following affinity tag is a 15 amino acid residue peptide which can bind to S-fragment of RNaseA? |
| A. | His-tag |
| B. | Myc-tag |
| C. | Calmodulin-binding peptide (CBP) |
| D. | S-tag |
| Answer» E. | |
| 5. |
Strep-tag is only used for the purification of recombinant proteins. |
| A. | True |
| B. | False |
| Answer» C. | |
| 6. |
Which of the following conserved consensus sequence is considered crucial for streptavidin-biotin binding? |
| A. | Ala-Gln-Gly |
| B. | Pro-His-Gln |
| C. | Gly-His-Gln |
| D. | His-Pro-Gln |
| Answer» E. | |
| 7. |
Which of the following is not true for streptavidin? |
| A. | It is extremely stable against proteases |
| B. | It is extremely stable against elevated temperatures |
| C. | It is devoid of sulfur-containing amino acids |
| D. | It is highly glycosylated |
| Answer» E. | |
| 8. |
Which of the following affinity tag is a 6 amino acid residue peptide which can be immobilized by transition metal ions? |
| A. | S-tag |
| B. | Myc tag |
| C. | Flag peptide |
| D. | His-tag |
| Answer» E. | |
| 9. |
Which of the following technique made it possible to shorten the laborious procedure of protein purification? |
| A. | Precipitation |
| B. | Column chromatography |
| C. | Adsorption/desorption |
| D. | Affinity chromatography |
| Answer» E. | |
| 10. |
Which of the following affinity tag is an 8 amino acid residue peptide that can be a target of monoclonal antibody? |
| A. | S-tag |
| B. | Myc tag |
| C. | His-tag |
| D. | Flag-peptide |
| Answer» E. | |
| 11. |
Which of the following is a ligand of streptavidin? |
| A. | Oxalic acid |
| B. | Pantothenic acid |
| C. | Zinc ion |
| D. | Biotin |
| Answer» E. | |
| 12. |
Which of the following affinity tag does not require harsh conditions for elution of the tagged protein from a column with the immobilized antibody? |
| A. | Flag-peptide |
| B. | His-tag |
| C. | Calmodulin-binding peptide (CBP) |
| D. | Strep-tag |
| Answer» E. | |
| 13. |
Which of the following is a short sequence of amino acids that is recognized as an epitope by a monoclonal antibody and is appended to a recombinant protein on the genetic level? |
| A. | Antigenic peptide |
| B. | Peptide tag |
| C. | Sequence tag |
| D. | Affinity tag |
| Answer» E. | |
| 14. |
Which of the following affinity tag is an 11 amino acid residue peptide that can be a target of monoclonal antibody? |
| A. | Flag-peptide |
| B. | S-tag |
| C. | His-tag |
| D. | Myc tag |
| Answer» E. | |
| 15. |
Which of the following is not associated with obtaining a pure protein? |
| A. | Precipitation |
| B. | Adsorption/desorption |
| C. | Column chromatography |
| D. | Solubilization |
| Answer» E. | |