

MCQOPTIONS
This section includes 19 Mcqs, each offering curated multiple-choice questions to sharpen your Biotechnology knowledge and support exam preparation. Choose a topic below to get started.
1. |
During which phase of growth of Penicillium chrysogenum maximum antibiotic production takes place |
A. | during the first phase |
B. | during the second phase |
C. | during the third phase |
D. | same in all the phases |
Answer» C. during the third phase | |
2. |
The yield of the antibiotic depends upon |
A. | pH of the medium |
B. | age of the inoculum |
C. | composition of the medium |
D. | all of these |
Answer» E. | |
3. |
Which of the following event occurs during second phase of growth of P. chrysogenum? |
A. | Synthesis of penicillin is high |
B. | Mycelial mass increases |
C. | pH increase |
D. | Both (a) and (b) |
Answer» E. | |
4. |
Inoculum preparation for the fermentation medium for penicillin takes place on |
A. | wheat seeds |
B. | barley seeds |
C. | rice seeds |
D. | all of these |
Answer» C. rice seeds | |
5. |
Vegetable oil, (corn oil or soybean oil) added in the fermentation medium for penicillin, act as |
A. | source of nutrients |
B. | carbon source |
C. | antifoam agent |
D. | both (a) and (c) |
Answer» D. both (a) and (c) | |
6. |
Which of the following organism(s) produce(s) tetracycline? |
A. | <i>Steptomyces aureofaciens</i> |
B. | <i>Steptomyces ramosus</i> |
C. | <i>Nocardia sulphurea</i> |
D. | All of these |
Answer» E. | |
7. |
High yield of chlorotetracyclin requires |
A. | no aeration |
B. | continuous aeration |
C. | aeration which does not affect the yield |
D. | controlled aeration |
Answer» C. aeration which does not affect the yield | |
8. |
At acidic pH, penicillin remains in |
A. | aqueous phase |
B. | solvent phase |
C. | both (a) and (b) |
D. | precipitates |
Answer» C. both (a) and (b) | |
9. |
Chlorotetracyclin is soluble in |
A. | organic solvents |
B. | water |
C. | ether |
D. | all of these |
Answer» C. ether | |
10. |
Penicillin is recovered after fermentation as |
A. | potassium penicillin |
B. | calcium penicillin |
C. | sodium penicillin |
D. | penicillin only |
Answer» B. calcium penicillin | |
11. |
pH of the fermentation medium for chlorotetracyclins is |
A. | 5-6 |
B. | 6-7 |
C. | 7-8 |
D. | 8-9 |
Answer» B. 6-7 | |
12. |
Antibiotics are typically produced in fed batch reactors because |
A. | antibiotic yields are generally higher when cells enter the stationary phase |
B. | the precursors are often toxic to the cells |
C. | antibiotic yields are generally higher when cell growth slows |
D. | All of the above |
Answer» E. | |
13. |
Which of the following event occurs during third phase of growth of P chrysogenum? |
A. | Concentration of antibiotic increases in the medium |
B. | Autolysis of the medium starts |
C. | Slight rise in pH due to liberation of ammonia |
D. | All of the above |
Answer» E. | |
14. |
At normal pH, penicillin remains in |
A. | aqueous phase |
B. | solvent phase |
C. | both (a) and (b) |
D. | precipitates |
Answer» B. solvent phase | |
15. |
Which of the following precursor is added in the medium to get penicillin G? |
A. | Phenyl carbamic acid |
B. | Phenyl acetic acid |
C. | Ammonium sulphate |
D. | Ammonium chloride |
Answer» C. Ammonium sulphate | |
16. |
Which of the following changes occur during first phase of growth of Penicillium chrysogenum? |
A. | Growth of mycelia occurs |
B. | Ammonia is liberated in the medium |
C. | Lactic acid present in the corn steep liquor is utilized at the maximum rate |
D. | All of the above |
Answer» E. | |
17. |
Penicillin is produced by |
A. | aerobic fermentation |
B. | anaerobic fermentation |
C. | aerobic fermentation followed by anaerobic fermentation |
D. | anaerobic fermentation followed by aerobic fermentation |
Answer» B. anaerobic fermentation | |
18. |
Media composition for the production of streptomycin is |
A. | soybean meal, glucose, peptone, malt extract, sodium chloride |
B. | soybean meal, glucose, peptone, malt extract, ammonium chloride |
C. | soybean meal, glucose, peptone, malt extract, calcium carbonate |
D. | soybean meal, glucose, peptone, malt extract, ammonium sulphate |
Answer» B. soybean meal, glucose, peptone, malt extract, ammonium chloride | |
19. |
Streptomycin is produced by |
A. | <i>S. griseus</i> |
B. | <i>S. griseoflavus</i> |
C. | <i>S. aerofaciens</i> |
D. | <i>S. ramosus</i> |
Answer» B. <i>S. griseoflavus</i> | |