Real time PCR utilises …… for detection and estimation of DNA products.
(a) methyl orange
(b) SYBR green
(c) phenolphthalein
(d) ethidium bromide
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Answer is : (b)
Real time PCR utilises SYBR green for detection and estimation of DNA products. SYBR green is a cyanine dye used to stain DNA. It binds with DNA molecules by intercalating between the DNA bases.