Which of the following statements is true about affinity chromatography?

Question

TuteeHUB · Accepted Answer

During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted last

TuteeHUB · Answer

During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted first

TuteeHUB · Answer

During the separation of a mixture of proteins, the protein which binds to ligand is eluted first

TuteeHUB · Answer

Unwanted proteins are eluted by ligand solution

1.	Which of the following statements is true about affinity chromatography?
A.	During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted first
B.	During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted last
C.	During the separation of a mixture of proteins, the protein which binds to ligand is eluted first
D.	Unwanted proteins are eluted by ligand solution
Answer» B. During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted last

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