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1. |
Which of the following is used to determine the appropriate stringency (Wallace rule)? |
A. | Tm = 2 x (No.of G:C base pair) + 4 x (No.of A:T base pair) |
B. | Tm = 4 x (No.of A:T base pair) + 2 x (No.of G:C base pair) |
C. | Tm = 4 x (No.of G:C base pair) + 2 x (No.of A:T base pair) |
D. | Tm = 69.3° + 0.41 (%)G+C -650/1 |
Answer» D. Tm = 69.3° + 0.41 (%)G+C -650/1 | |