Which of the following is true about Michaelis-Menten kinetics?

• A. Kₘ, the Michaelis constant, is defined as that concentration of substrate at which enzyme is working at maximum velocity
• B. It describes single substrate enzymes
• C. Kₘ, the Michaelis constant is defined as the dissociation constant of the enzyme-substrate complex
• D. It assumes covalent binding occurs between enzyme and substrate