A complete restriction digestion of a circular plasmid (5000bp) was carried out with HindIII, BamHI and EcoRIindividually. Restriction digestion yielded following fragments. Plasmid + HindIII 1200bp and3800bp Plasmid + BamHI 5000bp Plasmid + EcoRI 2500bp The number of sites for EcoRI, BamHI-andHindIIIpresent on this plasmid are

A complete restriction digestion of a circular plasmid (5000bp) was ca

1.	A complete restriction digestion of a circular plasmid (5000bp) was carried out with HindIII, BamHI and EcoRIindividually. Restriction digestion yielded following fragments. Plasmid + HindIII 1200bp and3800bp Plasmid + BamHI 5000bp Plasmid + EcoRI 2500bp The number of sites for EcoRI, BamHI-andHindIIIpresent on this plasmid are
A.	EcoRI-2, BamHI-1, HindIII-2
B.	EcoRI-1, BamHI-1, HindIII-2
C.	EcoRI-3, BamHI-2, HindIII-1
D.	EcoRI-2, BamHI-2, HindIII-1
Answer» B. EcoRI-1, BamHI-1, HindIII-2

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