What would happen if serum is omitted from the ELISA, but all other st – McqOptions

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1.	What would happen if serum is omitted from the ELISA, but all other steps remain same?
A.	Anti-human Ig-conjugate would not bind and be washed away
B.	The OD values would be nearly the same as the assay control
C.	Both (a) and (b)
D.	Anti-human Ig-conjugate would bind non-specifically to the ELISA plate
Answer» D. Anti-human Ig-conjugate would bind non-specifically to the ELISA plate

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