Enzyme is immobilised in 8 mm diameter agarose beads at a concentration of 0.018 kg protein m-3 gel. Ten beads are immersed in a well-mixed solution containing 3.2 10-3 kg m-3 substrate. The effective diffusivity of substrate in agarose gel is 2.1 10-9 m2 s-1. Kinetics of the enzyme can be approximated as first order with specific rate constant 3.11 105 s 1 per kg protein. Mass transfer effects outside the particles are negligible. Estimate the volume per bead.

Enzyme is immobilised in 8 mm diameter agarose beads at a concentratio

1.	Enzyme is immobilised in 8 mm diameter agarose beads at a concentration of 0.018 kg protein m^-3 gel. Ten beads are immersed in a well-mixed solution containing 3.2 10^-3 kg m^-3 substrate. The effective diffusivity of substrate in agarose gel is 2.1 10^-9 m² s^-1. Kinetics of the enzyme can be approximated as first order with specific rate constant 3.11 10⁵ s¹ per kg protein. Mass transfer effects outside the particles are negligible. Estimate the volume per bead.
A.	2.65 10<sup>-7</sup> m<sup>3</sup>
B.	2.66 10<sup>-7</sup> m<sup>3</sup>
C.	2.67 10<sup>-7</sup> m<sup>3</sup>
D.	2.68 10<sup>-7</sup> m<sup>3</sup>
Answer» E.