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This section includes 5 Mcqs, each offering curated multiple-choice questions to sharpen your Biochemistry knowledge and support exam preparation. Choose a topic below to get started.
1. |
Which of the following statements is true about two-dimensional electrophoresis? |
A. | Separates proteins of identical molecular weight, same pI but different charge |
B. | Separates proteins of different molecular weight and different pI |
C. | Separates proteins of identical molecular weight that differ in pI |
D. | Isoelectric focusing is also termed as two-dimensional electrophoresis |
Answer» D. Isoelectric focusing is also termed as two-dimensional electrophoresis | |
2. |
Which of the following statements is true about affinity chromatography? |
A. | During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted first |
B. | During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted last |
C. | During the separation of a mixture of proteins, the protein which binds to ligand is eluted first |
D. | Unwanted proteins are eluted by ligand solution |
Answer» B. During the separation of a mixture of proteins, the protein which does not bind to ligand is eluted last | |
3. |
The pattern on paper in chromatography is called __________ |
A. | Chroming |
B. | Chroma |
C. | Chromatograph |
D. | Chromatogram |
Answer» E. | |
4. |
Mobile phase can be ___________ |
A. | Only solid |
B. | Only gas |
C. | Solid or liquid |
D. | Liquid or gas |
Answer» E. | |
5. |
The salt which produces salting out effect during extraction of proteins is? |
A. | NH<sub>4</sub> SO<sub>4</sub> |
B. | (NH<sub>4</sub>)<sub>2</sub> SO<sub>4</sub> |
C. | (NH<sub>4</sub>)<sub>3</sub> SO<sub>4</sub> |
D. | NaCl |
Answer» C. (NH<sub>4</sub>)<sub>3</sub> SO<sub>4</sub> | |