Explore topic-wise MCQs in Biochemistry.

This section includes 76 Mcqs, each offering curated multiple-choice questions to sharpen your Biochemistry knowledge and support exam preparation. Choose a topic below to get started.

1.

The dissociation constant of H2O at 25° C is

A. 10-14 M
B. 1014 M
C. 10-7 M
D. 107 M
Answer» B. 1014 M
2.

Which among the following statements is true about the following chemical reaction?3Fe(s) + 4H₂O → Fe₃O₄ (s) + 4H₂

A. Iron metal is getting reduced
B. Water is acting as a reducing agent
C. Water is acting as an oxidizing agent
D. Water is getting oxidized
Answer» D. Water is getting oxidized
3.

If pH of solution of NaOH is 12.0 the pH of H₂SO₄ solution of same molarity will be?

A. 2.0
B. 12.0
C. 1.7
D. 10.0387
Answer» D. 10.0387
4.

Calculate the pKₐ of lactic acid, given that when the concentration of lactic acid is 0.010M and the concentration of lactate is 0.087M, the pH is 4.80.

A. 4.0
B. 3.9
C. 3.3
D. 4.1
Answer» C. 3.3
5.

Which of the following does not react with water?

A. Barium
B. Calcium
C. Beryllium
D. Strontium
Answer» D. Strontium
6.

Identify the products formed in the following reaction.CH₃COOCH₃ + H₂O → A + B

A. CH₃COOH and CH₃OH
B. CH₃COOOH and CH₄
C. CO₂ and C₂H₆
D. No reaction
Answer» B. CH₃COOOH and CH₄
7.

Which can act as buffer?

A. NH₄Cl + HCl
B. CH₃COOH + H₂CO₃
C. 40ml of 0.1M NaCN + 20ml of 0.1M HCN
D. NaCl + NaOH
Answer» D. NaCl + NaOH
8.

Assertion A: pH of a buffer solution solution does not change on dilution.Reason R: On dilution the ration of concentration of salt and acid (or base) remains unchanged.

A. A and R are true, R is the correct explanation of A
B. A and R are true, R is not correct explanation of A
C. A is true but R is false
D. A is false but R is true
Answer» B. A and R are true, R is not correct explanation of A
9.

Hydrolysis reactions are catalyzed by enzymes called ___________

A. Oxidoreductase
B. Ligase
C. Hydrolase
D. Isomerase
Answer» D. Isomerase
10.

Calculate the pH of a mixture of 0.10M acetic acid and 0.20M sodium acetate. The pKₐ of acetic acid is 4.76.

A. 5.1
B. 4.1
C. 6.1
D. 7.1
Answer» B. 4.1
11.

Which of the following is the correct equation?

A. CO₂ + H₂O → H₂CO₃
B. CO₂ + H₂O → HCO₃
C. CO₂ + H₂O → H₃CO₃
D. CO₂ + H₂O → H₂CO₂
Answer» B. CO₂ + H₂O → HCO₃
12.

What does it mean when we say that water acts as a “heat buffer”?

A. It keeps the temperature of an organism relatively constant as the temperature of the surroundings fluctuates
B. It increases the temperature of an organism as the temperature of the surroundings increases
C. It decreases the temperature of an organism as the temperature of the surroundings increases
D. It increases the temperature of an organism as the temperature of the surroundings decreases
Answer» B. It increases the temperature of an organism as the temperature of the surroundings increases
13.

Consider the following reaction, in the presence of light2H₂O + 2X → O₂ + 2XH₂Which of the following statements is true?

A. X is electron-accepting specie
B. X is electron-donating specie
C. Water is an electron acceptor
D. Water neither donates nor accepts any electron
Answer» B. X is electron-donating specie
14.

The buffer capacity is equal to __________

A. Δn / ΔpH
B. pH / Δn
C. ± 1pKₐ
D. ± 2pKₐ
Answer» B. pH / Δn
15.

Which of the following mixture in aqueous solution of equimolar concentration acts as a buffer solution?

A. HNO₃ + NaOH
B. H₂SO₄ + KOH
C. NH₄OH(excess) + HCl
D. CH₃COOH + NaOH(excess)
Answer» D. CH₃COOH + NaOH(excess)
16.

Calculate the ratio of the concentrations of acetate and acetic acid required in a buffer system of pH 5.30.

A. 3.2
B. 3.3
C. 3.4
D. 3.5
Answer» E.
17.

Which of the following is not a condensation reaction?

A. ADP + inorganic phosphate → ATP + H₂O
B. R-OH + inorganic phosphate → phosphate ester + H₂O
C. R-COOH + inorganic phosphate → Acyl phosphate + H₂O
D. 6CO₂ + 6H₂O → C₆H₁₂O₆ + 6CO₂
Answer» E.
18.

Tertiary conformation of proteins is maintained by 3 types of bonds namely ionic, hydrogen and ____________

A. Sulfide
B. Disulfide
C. Covalent
D. Peptide
Answer» C. Covalent
19.

What trend does the reactivity of group II elements follow with water?

A. Increases down the group
B. Decreases down the group
C. Does not follow any particular trend
D. No reaction
Answer» B. Decreases down the group
20.

Buffer capacity is maximum when __________

A. One mole of NH₄Cl is added to two moles of NH₄OH
B. One mole of NH₄Cl is added to one moles of NH₄OH
C. One mole of NH₄Cl is added to one mole of NaOH
D. One mole of NaCl is added to one mole of NaOH
Answer» C. One mole of NH₄Cl is added to one mole of NaOH
21.

1M NaCl and 1M HCl are present in an aqueous solution. What is the solution?

A. Not a buffer solution with pH < 7
B. Not a buffer solution with pH > 7
C. A buffer solution with pH < 7
D. A buffer solution with pH > 7
Answer» B. Not a buffer solution with pH > 7
22.

Which of the following is a hypotonic solution?

A. 10% dextrose in water
B. 0.45% sodium chloride
C. 5% dextrose in 0.9% sodium chloride
D. Oceans
Answer» C. 5% dextrose in 0.9% sodium chloride
23.

1M NaCl and 1M HCl are present in an aqueous solution. The solution is ____________

A. Not a buffer solution with pH < 7
B. Not a buffer solution with pH > 7
C. A buffer solution with pH < 7
D. A buffer solution with pH > 7
Answer» B. Not a buffer solution with pH > 7
24.

The bond dissociation energy of hydrogen bonds in a water molecule is ______________

A. 470 kJ/mole
B. 470 J/mole
C. 23 kJ/mole
D. 23 J/mole
Answer» D. 23 J/mole
25.

Which of the following is an isotonic solution?

A. 0.9% sodium chloride
B. 0.45% sodium chloride
C. Oceans
D. 10% dextrose in water
Answer» B. 0.45% sodium chloride
26.

The bond dissociation energy of O-H bond in a water molecule is _____________

A. 23 kJ/mole
B. 470 kJ/mole
C. 470 J/mole
D. 23 J/mole
Answer» C. 470 J/mole
27.

Which of the following acids has the lowest pKₐ value?

A. Acetic acid
B. Sulphuric acid
C. Dil.HCl
D. Oxalic acid
Answer» C. Dil.HCl
28.

What is the concentration of OH⁻ in a solution with a H⁺ concentration of 1.3×10⁻⁴ M?

A. 7.7×10⁻¹⁰ M
B. 7.7×10⁻⁹ M
C. 7.7×10⁻¹¹ M
D. 7.7×10⁻¹² M
Answer» D. 7.7×10⁻¹² M
29.

Which of the following bases has the highest pKₐ value?

A. NaOH
B. NaNO₃
C. KNO₃
D. KCl
Answer» B. NaNO₃
30.

K1 and K2 for oxalic acid are 6.5×10⁻² and 6.1×10⁻⁵ respectively. What will be the [OH⁻] in a 0.01M solution of sodium oxalate?

A. 9.6×10⁻⁶
B. 1.4×10⁻¹
C. 1.2×10⁻⁶
D. 1.3×10⁻⁸
Answer» D. 1.3×10⁻⁸
31.

If pKb for fluoride at 25°c is 10.83, the ionization constant of hydrofluoric acid in water at this temperature is?

A. 3.52×10⁻³
B. 6.75×10⁻⁴
C. 5.38×10⁻²
D. 1.74×10⁻⁵
Answer» C. 5.38×10⁻²
32.

The hydrolysis constant of CH₃COONa is given by

A. Kh = Kw/Ka
B. Kh = Kw/Kb
C. Kh = Kw/Ka×Kb
D. Kh = Ka + Kb
Answer» B. Kh = Kw/Kb
33.

The degree of ionization does not depend on?

A. Temperature
B. Current
C. Nature of solvent
D. Concentration
Answer» C. Nature of solvent
34.

Which of the following acid/base pairs act as natural buffers in living systems?

A. H₂CO₃/HCO₃¯
B. H₂PO₄-/HPO₄²¯
C. Histidine+/histidine
D. All of these
Answer» E.
35.

What is the concentration, in moles/liter, of the hydrogen ion, if pH of a solution is 7?

A. 7
B. 7 x 10^-7
C. 5 x 10^-7
D. 1 x 10^-7
Answer» E.
36.

Which of the following alcohols would be most soluble in water?

A. methanol
B. ethanol
C. butanol
D. octanol
Answer» B. ethanol
37.

Which of the following is a hypertonic solution?

A. Oceans
B. Freshwater habitats
C. 0.45% sodium chloride
D. Red blood cells placed in fresh water
Answer» B. Freshwater habitats
38.

The processes i and ii are respectively.i) CuSO₄ + 5H₂O → CuSO₄.5H₂Oii) PCl₃ + 3H₂O → H₃PO₃ + 3HCl

A. Hydration and dehydration
B. Hydration and hydrolysis
C. Hydrolysis and hydrolysis
D. Hydration and hydration
Answer» C. Hydrolysis and hydrolysis
39.

Aqueous solution of the detergents are _________

A. Neutral
B. Acidic
C. Basic
D. Amphoteric
Answer» D. Amphoteric
40.

A compound that is capable of forming hydrogen bonds with water

A. is probably held together by noncovalent bonds
B. contains at least some polar covalent bonds
C. should act as a good buffer for acids and bases
D. does not dissolve well in water
Answer» C. should act as a good buffer for acids and bases
41.

For an acid buffer solution the pH is 3. The pH can be increased by ____________

A. Increasing the concentration of salt
B. Increasing the concentration of acid
C. Decreasing the concentration of salt
D. Independent of concentration of acid & salt
Answer» B. Increasing the concentration of acid
42.

What is the heat of vaporization of water?

A. 2260 J/g
B. 2260 kJ/g
C. 1260 J/g
D. 1260 kJ/g
Answer» B. 2260 kJ/g
43.

If pH of solution of NaOH is 12.0 the pH of H₂SO₄ solution of same molarity will be ____________

A. 2.0
B. 12.0
C. 1.7
D. 10.0387
Answer» D. 10.0387
44.

Most of the important functional groups in biological molecules contain

A. oxygen and/or nitrogen and are acidic
B. oxygen and a phosphate
C. nitrogen and a phosphate
D. oxygen and/or nitrogen and are polar
Answer» E.
45.

If protein is made exclusively from nonpolar amino acids, then it will this protein interact with water?

A. be repelled by water
B. It will be attracted to water
C. It will form a membrane in water
D. It will act as a buffer and stabilize the pH
Answer» B. It will be attracted to water
46.

Identify the triprotic acid from the following.

A. Carbonic acid
B. Bicarbonate
C. Glycine
D. Phosphoric acid
Answer» E.
47.

What is the factor that is responsible for salts like NaCl to dissolve in water easily?

A. Decrease in entropy
B. Increase in entropy
C. Increase in enthalpy
D. Decrease in enthalpy
Answer» C. Increase in enthalpy
48.

ΔG is negative for which of the following processes?

A. Melting of ice and evaporation of water
B. Oxidation of nitrogen
C. Oxidation of gold
D. Burning of chlorine
Answer» B. Oxidation of nitrogen
49.

What makes water a liquid at room temperature?

A. Hydrogen bonds between water molecules
B. Covalent bonding
C. Noncovalent interactions
D. Van der Waals forces of attraction
Answer» B. Covalent bonding
50.

ΔH is positive for which of the following processes?

A. Oxidation of nitrogen
B. Melting of ice and evaporation of water
C. Oxidation of gold
D. Burning of chlorine
Answer» C. Oxidation of gold