In an experiment, you wanted to see which mutation will have worse effect on transcription. In the 1st set you deleted 94 amino acids from the C-terminus and in the 2nd set you replace the 265th C-terminal arginine to cystine. Which in your opinion will have the worst effect?

Both will have similar negative effect

No visible effect on rate of transcription

In an experiment you add increasing amount of sigma factor to a mixture of DNA fragment and core polymerase in vitro. You also add [14C]ATP and [γ-32P]ATP in the mixture and check the rate of incorporation. Results show that the incorporation of both of this labels increase on adding more sigma factor. What will you conclude from your observation?

Sigma confers specificity and increases transcription rate

Sigma increases initiation and elongation rate

Sigma increases initiation of transcription rate

Which of these is the 1st event to take place during transcription initiation?

Formation of a closed initiation complex

Formation of open initiation complex

Formation of absorptive transcript

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1.	In an experiment, you wanted to see which mutation will have worse effect on transcription. In the 1st set you deleted 94 amino acids from the C-terminus and in the 2nd set you replace the 265th C-terminal arginine to cystine. Which in your opinion will have the worst effect?
A.	94 amino acid mutant
B.	265th amino acid mutant
C.	Both will have similar negative effect
D.	No visible effect on rate of transcription
Answer» D. No visible effect on rate of transcription

Discussion

2.	In an experiment you add increasing amount of sigma factor to a mixture of DNA fragment and core polymerase in vitro. You also add [14C]ATP and [γ-32P]ATP in the mixture and check the rate of incorporation. Results show that the incorporation of both of this labels increase on adding more sigma factor. What will you conclude from your observation?
A.	Sigma confers specificity and increases transcription rate
B.	Sigma increases initiation and elongation rate
C.	Sigma increases initiation of transcription rate
D.	Sigma causes faster elongation
Answer» C. Sigma increases initiation of transcription rate

Discussion

3.	Which of these is the 1st event to take place during transcription initiation?
A.	Formation of a closed initiation complex
B.	Formation of open initiation complex
C.	Formation of absorptive transcript
D.	Promoter clearance
Answer» C. Formation of absorptive transcript

Discussion