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This section includes 5 Mcqs, each offering curated multiple-choice questions to sharpen your Molecular Biology Questions and Answers knowledge and support exam preparation. Choose a topic below to get started.
1. |
________________ is a modification of RT – PCR. |
A. | Overlap extension PCR |
B. | Inverse PCR |
C. | Thermal cycle sequencing PCR |
D. | RACE |
Answer» E. | |
2. |
The cDNA library provides several advantages over RT – PCR. |
A. | True |
B. | False |
Answer» C. | |
3. |
The digestion of mRNA during RT – PCR is carried out by the enzyme ____________ |
A. | Exonuclease |
B. | RNase H |
C. | Bal 31 |
D. | Endonuclease |
Answer» C. Bal 31 | |
4. |
In RT – PCR the enzyme deoxynucleotydil transferase adds poly – G residues in the __________ |
A. | 5’ end of RNA |
B. | 3’ end of RNA |
C. | 3’ end of cDNA |
D. | 5’ end of cDNA |
Answer» D. 5’ end of cDNA | |
5. |
Which of the following serves as the first primer in RT – PCR for eukaryotic RNA? |
A. | Oligo A |
B. | Oligo T |
C. | Oligo G |
D. | Oligo C |
Answer» C. Oligo G | |