The RP13 gene of chromosome 17 codes for a protein __________ .

involved in the determination of personality

that is a component of hair and nails

involved in the determination of personality

Isoschizomers recognize

same recognition site and different recognition sequence

same recognition sequence but different recognition site

same recognition site and recognition sequence

same recognition site and different recognition sequence

different recognition site and different recognition sequence

An example of a restriction fragment length polymorphism is

cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved

an Eco RI cuts DNA at a different sequence than Hind III

different length fragments of DNA resulting from loss or gain of a restriction site

cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved

always contains an origin of replication

usually contains a gene that confers antibiotic resistance to the bacterial host

always contains DNA segments for the regulation of mRNA production

Knockout mice are created by

mutagenizing a mouse and selecting for mutant offspring

creating a chimera by fusing cells from two different cell lines

infecting the mouse with a retrovirus

transfecting embryonic stem cells with an altered gene sequence

always contains an origin of replication

usually contains one or more restriction sites

The order for the construction of a cDNA fragment from mRNA is to

treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT

bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase

treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT

digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase

bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase

the formation of thymine dimers

ionization of water in the cell

Which of the following is not true of small inbreeding populations?

They tend to loose genetic diversity

There is an increased incidence of recessive diseases

Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

PCR-amplified variable numbers of tandem repeats (VNTRs)

copies of the gene with an altered sequence so that a restriction site is inserted

short sequences that will hybridize only to a specific base sequence

PCR-amplified variable numbers of tandem repeats (VNTRs)

Under which of the following conditions would population gene frequencies remain the same?

Active migration between groups

The TP53 gene of chromosome 17 codes for a protein __________ .

that is like a white blood cell protein

that plays a role in the digestive process

involved in the regulation of the cell cycle

that is like a white blood cell protein

The transfer of antibiotic-resistant genes from genetically engineered bacteria to disease-causing bacteria __________ .

would be of no concern if it occurred.

Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because

in both cases molecules migrate to the anode

both techniques rely on a constant charge to mass ratio

both techniques utilize the sieving properties of gels

Which of the following is correct in terms of determination of location of genetic traits?

Restriction sites allow DNAs to be digested

Known protein coding sequences are too far apart to allow linkage determination for most new genes

Restriction sites allow DNAs to be digested

Protein-coding genes are always associated with a restriction pattern

protect bacteria from viral infection

Restriction enzymes are named for

the bacterium they are derived from

cannot be used to obtain human genes because it would be radioactive

contains DNA for specific human proteins

contains DNA for virtually all of the human proteins in vectors

cannot be used to obtain human genes because it would be radioactive

In order to insert a foreign gene into a plasmid, both must __________

originate from the same type of cell

be cut by the same restriction enzyme

32 + Mcqs in Recombinant DNA Technology in Biochemistry Page 1 McqOptions

1.	The RP13 gene of chromosome 17 codes for a protein __________ .
A.	involved in glucose transport
B.	that is a component of hair and nails
C.	involved in eye development
D.	involved in the determination of personality
Answer» D. involved in the determination of personality

Discussion

2.	Isoschizomers recognize
A.	same recognition sequence but different recognition site
B.	same recognition site and recognition sequence
C.	same recognition site and different recognition sequence
D.	different recognition site and different recognition sequence
Answer» C. same recognition site and different recognition sequence

Discussion

3.	The unpaired nucleotides produced by the action of restriction enzymes are referred to have
A.	sticky ends
B.	single strands
C.	restriction fragments
D.	ligases
Answer» B. single strands

Discussion

4.	Which type of restriction enzymes do not usually require ATP?
A.	Type I
B.	Type II
C.	Type III
D.	Type IV
Answer» C. Type III

Discussion

5.	An example of a restriction fragment length polymorphism is
A.	an Eco RI cuts DNA at a different sequence than Hind III
B.	different length fragments of DNA resulting from loss or gain of a restriction site
C.	cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved
D.	all of the above
Answer» C. cystic fibrosis results from a three base deletion in most cases but in other cases, other mutations are involved

Discussion

6.	An expression vector
A.	always contains an origin of replication
B.	usually contains a gene that confers antibiotic resistance to the bacterial host
C.	always contains DNA segments for the regulation of mRNA production
D.	all of the above
Answer» E.

Discussion

7.	Knockout mice are created by
A.	mutagenizing a mouse and selecting for mutant offspring
B.	creating a chimera by fusing cells from two different cell lines
C.	infecting the mouse with a retrovirus
D.	transfecting embryonic stem cells with an altered gene sequence
Answer» E.

Discussion

8.	A plasmid
A.	is a circular DNA molecule
B.	always contains an origin of replication
C.	usually contains one or more restriction sites
D.	all of the above
Answer» E.

Discussion

9.	The order for the construction of a cDNA fragment from mRNA is to
A.	bind oligo-dT, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase
B.	treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT
C.	digest with RNase, add G residues to the 3' end, treat with reverse transcriptase, add G residues to the 3' end and treat with DNA polymerase
D.	bind oligo-dC, treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dT and treat with DNA polymerase
Answer» B. treat with reverse transcriptase, digest with RNase, add G residues to the 3' end, bind oligo-dC, treat with DNA polymerase and bind oligo-dT

Discussion

10.	Which of the following can be diagnosed by amniocentesis?
A.	Down's syndrome
B.	Cystic Fibrosis
C.	Sickle cell anemia
D.	All of these
Answer» E.

Discussion

11.	Which of the following genetic diseases would be amenable to genetic engineering?
A.	Down's syndrome
B.	Muscular dystrophy
C.	Cystic fibrosis
D.	Cri du Chat
Answer» D. Cri du Chat

Discussion

12.	X-rays cause
A.	the formation of thymine dimers
B.	ionization of water in the cell
C.	heat
D.	none of the above
Answer» C. heat

Discussion

13.	What enzyme forms covalent bonds between restriction fragments?
A.	DNA primase
B.	DNA helicase
C.	DNA polymerase
D.	DNA ligase
Answer» E.

Discussion

14.	Which of the following is not true of small inbreeding populations?
A.	They tend to loose genetic diversity
B.	There is an increased incidence of recessive diseases
C.	Alleles may become fixed
D.	Mutation is increased
Answer» E.

Discussion

15.	Which of these genes codes for a protein that plays a role in growth?
A.	DCP1
B.	SCLC6A4
C.	KRTHA1
D.	GH1
Answer» E.

Discussion

16.	Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are
A.	copies of the gene with an altered sequence so that a restriction site is inserted
B.	mutagenized copies of a gene
C.	short sequences that will hybridize only to a specific base sequence
D.	PCR-amplified variable numbers of tandem repeats (VNTRs)
Answer» D. PCR-amplified variable numbers of tandem repeats (VNTRs)

Discussion

17.	Under which of the following conditions would population gene frequencies remain the same?
A.	Selection for homozygotes
B.	Small population size
C.	Active migration between groups
D.	Random mating
Answer» E.

Discussion

18.	In gel electrophoresis, DNA molecules migrate from __________ to __________ ends of the gel.
A.	negative ... positive
B.	basic ... acidic
C.	long ... short
D.	positive to negative
Answer» B. basic ... acidic

Discussion

19.	First discovered, Type II restriction endonuclease was
A.	Hinf I
B.	Eco K
C.	Hind II
D.	EcoRI
Answer» D. EcoRI

Discussion

20.	The TP53 gene of chromosome 17 codes for a protein __________ .
A.	that plays a role in the digestive process
B.	involved in glucose transport
C.	involved in the regulation of the cell cycle
D.	that is like a white blood cell protein
Answer» D. that is like a white blood cell protein

Discussion

21.	Which of the following techniques can be used to determine the defective gene and for developing cancer?
A.	Western blot
B.	Southern blot
C.	Northern blot
D.	Eastern blot
Answer» C. Northern blot

Discussion

22.	The transfer of antibiotic-resistant genes from genetically engineered bacteria to disease-causing bacteria __________ .
A.	would be of no concern if it occurred.
B.	has occurred
C.	can never occur
D.	seems unlikely
Answer» E.

Discussion

23.	Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because
A.	in both cases molecules migrate to the anode
B.	both techniques rely on a constant charge to mass ratio
C.	both techniques utilize the sieving properties of gels
D.	all of the above
Answer» E.

Discussion

24.	Which of the following is correct in terms of determination of location of genetic traits?
A.	Known protein coding sequences are too far apart to allow linkage determination for most new genes
B.	Restriction sites allow DNAs to be digested
C.	Protein-coding genes are always associated with a restriction pattern
D.	None of the above
Answer» B. Restriction sites allow DNAs to be digested

Discussion

25.	Restriction enzymes
A.	protect bacteria from viral infection
B.	cut DNA in a staggered fashion
C.	cut DNAs producing a blunt end
D.	all of the above
Answer» E.

Discussion

26.	Which of these genes codes for a protein that plays a role in white blood cell function?
A.	DCP1
B.	MPO
C.	GLUT4
D.	RP13
Answer» C. GLUT4

Discussion

27.	Restriction enzymes are named for
A.	the person who discovered
B.	the bacterium they are derived from
C.	the viral DNA that they attack
D.	none of the above
Answer» C. the viral DNA that they attack

Discussion

28.	A human cDNA library
A.	contains DNA for specific human proteins
B.	contains DNA for virtually all of the human proteins in vectors
C.	cannot be used to obtain human genes because it would be radioactive
D.	none of the above
Answer» C. cannot be used to obtain human genes because it would be radioactive

Discussion

29.	When populations are small, gene frequencies can change from generation to generation and some alleles may become fixed in a population. This is called __________ .
A.	assortative mating
B.	inbreeding
C.	heterosis
D.	genetic drift
Answer» E.

Discussion

30.	Which of these restriction enzymes produce blunt ends?
A.	SaII
B.	EcoRV
C.	XhoI
D.	HindIII
Answer» C. XhoI

Discussion

31.	In order to insert a foreign gene into a plasmid, both must __________
A.	have identical DNA sequences
B.	originate from the same type of cell
C.	be cut by the same restriction enzyme
D.	be of the same length
Answer» D. be of the same length

Discussion

32.	Which of the following pair will produce complementary sticky ends?
A.	Eco RI & MspI
B.	Msp I & HPA II
C.	Sau 3A & Bam HI
D.	Mbo I & Sau 3A
Answer» D. Mbo I & Sau 3A

Discussion

Explore topic-wise MCQs in Biochemistry.

The RP13 gene of chromosome 17 codes for a protein __________ .

Isoschizomers recognize

The unpaired nucleotides produced by the action of restriction enzymes are referred to have

Which type of restriction enzymes do not usually require ATP?

An example of a restriction fragment length polymorphism is

An expression vector

Knockout mice are created by

A plasmid

The order for the construction of a cDNA fragment from mRNA is to

Which of the following can be diagnosed by amniocentesis?

Which of the following genetic diseases would be amenable to genetic engineering?

X-rays cause

What enzyme forms covalent bonds between restriction fragments?

Which of the following is not true of small inbreeding populations?

Which of these genes codes for a protein that plays a role in growth?

Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

Under which of the following conditions would population gene frequencies remain the same?

In gel electrophoresis, DNA molecules migrate from to ends of the gel.

First discovered, Type II restriction endonuclease was

The TP53 gene of chromosome 17 codes for a protein __________ .

Which of the following techniques can be used to determine the defective gene and for developing cancer?

The transfer of antibiotic-resistant genes from genetically engineered bacteria to disease-causing bacteria __________ .

Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because

Which of the following is correct in terms of determination of location of genetic traits?

Restriction enzymes

Which of these genes codes for a protein that plays a role in white blood cell function?

Restriction enzymes are named for

A human cDNA library

When populations are small, gene frequencies can change from generation to generation and some alleles may become fixed in a population. This is called __________ .

Which of these restriction enzymes produce blunt ends?

In order to insert a foreign gene into a plasmid, both must __________

Which of the following pair will produce complementary sticky ends?

Explore topic-wise MCQs in Biochemistry.

The RP13 gene of chromosome 17 codes for a protein __________ .

Isoschizomers recognize

The unpaired nucleotides produced by the action of restriction enzymes are referred to have

Which type of restriction enzymes do not usually require ATP?

An example of a restriction fragment length polymorphism is

An expression vector

Knockout mice are created by

A plasmid

The order for the construction of a cDNA fragment from mRNA is to

Which of the following can be diagnosed by amniocentesis?

Which of the following genetic diseases would be amenable to genetic engineering?

X-rays cause

What enzyme forms covalent bonds between restriction fragments?

Which of the following is not true of small inbreeding populations?

Which of these genes codes for a protein that plays a role in growth?

Some genetic diseases cannot be diagnosed by changes in restriction sites. Some of these can be detected by allele-specific oligonucleotide probes. These are

Under which of the following conditions would population gene frequencies remain the same?

In gel electrophoresis, DNA molecules migrate from __________ to __________ ends of the gel.

First discovered, Type II restriction endonuclease was

The TP53 gene of chromosome 17 codes for a protein __________ .

Which of the following techniques can be used to determine the defective gene and for developing cancer?

The transfer of antibiotic-resistant genes from genetically engineered bacteria to disease-causing bacteria __________ .

Both DNA gel electrophoresis and SDS-PAGE of proteins are similar because

Which of the following is correct in terms of determination of location of genetic traits?

Restriction enzymes

Which of these genes codes for a protein that plays a role in white blood cell function?

Restriction enzymes are named for

A human cDNA library

When populations are small, gene frequencies can change from generation to generation and some alleles may become fixed in a population. This is called __________ .

Which of these restriction enzymes produce blunt ends?

In order to insert a foreign gene into a plasmid, both must __________

Which of the following pair will produce complementary sticky ends?

In gel electrophoresis, DNA molecules migrate from to ends of the gel.