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This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Biochemistry knowledge and support exam preparation. Choose a topic below to get started.
1. |
Which of the following is Edman reagent? |
A. | Phenylisothiocyanate |
B. | CF3 COOH |
C. | FDNB |
D. | Phenylthiocarbonyl |
Answer» B. CF3 COOH | |
2. |
What best summarizes the MALDI method by which gas phase ions are produced for mass spectrometry? |
A. | Sample is hit by a low energy xenon beam |
B. | Sample is forced through a narrow capillary tube and the solvent rapidly evaporates |
C. | Sample is embedded in a crystalline matrix and bombarded by laser beams |
D. | Sample is heated and then bombarded by electrons |
Answer» D. Sample is heated and then bombarded by electrons | |
3. |
Edman degradation is used for _________ |
A. | Identifying N-terminal amino acids |
B. | Identifying C-terminal amino acids |
C. | Identifying amino acid |
D. | Identifying carbohydrates |
Answer» B. Identifying C-terminal amino acids | |
4. |
Cleaving of peptide chain is done by _________ |
A. | Trypsin |
B. | Tyrosine |
C. | Tryptophan |
D. | Arginine |
Answer» B. Tyrosine | |
5. |
The amino acid sequences of thousands of different proteins from many species have been determined using principles first developed by? |
A. | Edman |
B. | Sanger |
C. | Mendel |
D. | Watson and Crick |
Answer» C. Mendel | |
6. |
Which of the following is a Sanger’s reagent? |
A. | 1-fluoro-2, 4-dinitrobenzene |
B. | 1-fluoro-2, 3-dinitrobenzene |
C. | 1-fluoro-2, 4-trinitrobenzene |
D. | 1-fluoro-2, 3-trinitrobenzene |
Answer» B. 1-fluoro-2, 3-dinitrobenzene | |
7. |
Amino acids sequence in DNA can be determined by the order of their _________ |
A. | rRNA |
B. | tRNA |
C. | Nucleotides |
D. | mRNA |
Answer» D. mRNA | |
8. |
Which_of_the_following_is_Edman_reagent?$ |
A. | Phenylisothiocyanate |
B. | CF<sub>3</sub> COOH |
C. | FDNB |
D. | Phenylthiocarbonyl |
Answer» B. CF<sub>3</sub> COOH | |
9. |
Edman degradation is used fo? |
A. | Identifying N-terminal amino acids |
B. | Identifying C-terminal amino acids |
C. | Identifying amino acid |
D. | Identifying carbohydrates |
Answer» B. Identifying C-terminal amino acids | |
10. |
Which of the following is the correct order of sequencing? |
A. | Cleaving, sequencing and ordering |
B. | Sequencing, ordering and cleaving |
C. | Ordering, cleaving and sequencing |
D. | Ordering, sequencing and cleaving |
Answer» B. Sequencing, ordering and cleaving | |
11. |
Cleaving of peptide chain is done by |
A. | Trypsin |
B. | Tyrosine |
C. | Tryptophan |
D. | Arginine |
Answer» B. Tyrosine | |
12. |
Which of the following compound is not involved in Edman degradation? |
A. | Phenylisothiocyanate |
B. | CF<sub>3</sub> COOH |
C. | FDNB |
D. | Phenylthiocarbonyl |
Answer» D. Phenylthiocarbonyl | |
13. |
The amino acid sequences of thousands of different proteins from many species have been determined using principles first developed by |
A. | Edman |
B. | Sanger |
C. | Mendel |
D. | Watson and Crick |
Answer» C. Mendel | |
14. |
Which of the following is a Sanger’s reagent?$ |
A. | 1-fluoro-2, 4-dinitrobenzene |
B. | 1-fluoro-2, 3-dinitrobenzene |
C. | 1-fluoro-2, 4-trinitrobenzene |
D. | 1-fluoro-2, 3-trinitrobenzene |
Answer» B. 1-fluoro-2, 3-dinitrobenzene | |
15. |
Amino acids sequence in DNA can be determined by the order of their |
A. | rRNA |
B. | tRNA |
C. | Nucleotides |
D. | mRNA |
Answer» D. mRNA | |