Explore topic-wise MCQs in Biochemistry.

This section includes 15 Mcqs, each offering curated multiple-choice questions to sharpen your Biochemistry knowledge and support exam preparation. Choose a topic below to get started.

1.

Which of the following is Edman reagent?

A. Phenylisothiocyanate
B. CF3 COOH
C. FDNB
D. Phenylthiocarbonyl
Answer» B. CF3 COOH
2.

What best summarizes the MALDI method by which gas phase ions are produced for mass spectrometry?

A. Sample is hit by a low energy xenon beam
B. Sample is forced through a narrow capillary tube and the solvent rapidly evaporates
C. Sample is embedded in a crystalline matrix and bombarded by laser beams
D. Sample is heated and then bombarded by electrons
Answer» D. Sample is heated and then bombarded by electrons
3.

Edman degradation is used for _________

A. Identifying N-terminal amino acids
B. Identifying C-terminal amino acids
C. Identifying amino acid
D. Identifying carbohydrates
Answer» B. Identifying C-terminal amino acids
4.

Cleaving of peptide chain is done by _________

A. Trypsin
B. Tyrosine
C. Tryptophan
D. Arginine
Answer» B. Tyrosine
5.

The amino acid sequences of thousands of different proteins from many species have been determined using principles first developed by?

A. Edman
B. Sanger
C. Mendel
D. Watson and Crick
Answer» C. Mendel
6.

Which of the following is a Sanger’s reagent?

A. 1-fluoro-2, 4-dinitrobenzene
B. 1-fluoro-2, 3-dinitrobenzene
C. 1-fluoro-2, 4-trinitrobenzene
D. 1-fluoro-2, 3-trinitrobenzene
Answer» B. 1-fluoro-2, 3-dinitrobenzene
7.

Amino acids sequence in DNA can be determined by the order of their _________

A. rRNA
B. tRNA
C. Nucleotides
D. mRNA
Answer» D. mRNA
8.

Which_of_the_following_is_Edman_reagent?$

A. Phenylisothiocyanate
B. CF<sub>3</sub> COOH
C. FDNB
D. Phenylthiocarbonyl
Answer» B. CF<sub>3</sub> COOH
9.

Edman degradation is used fo?

A. Identifying N-terminal amino acids
B. Identifying C-terminal amino acids
C. Identifying amino acid
D. Identifying carbohydrates
Answer» B. Identifying C-terminal amino acids
10.

Which of the following is the correct order of sequencing?

A. Cleaving, sequencing and ordering
B. Sequencing, ordering and cleaving
C. Ordering, cleaving and sequencing
D. Ordering, sequencing and cleaving
Answer» B. Sequencing, ordering and cleaving
11.

Cleaving of peptide chain is done by

A. Trypsin
B. Tyrosine
C. Tryptophan
D. Arginine
Answer» B. Tyrosine
12.

Which of the following compound is not involved in Edman degradation?

A. Phenylisothiocyanate
B. CF<sub>3</sub> COOH
C. FDNB
D. Phenylthiocarbonyl
Answer» D. Phenylthiocarbonyl
13.

The amino acid sequences of thousands of different proteins from many species have been determined using principles first developed by

A. Edman
B. Sanger
C. Mendel
D. Watson and Crick
Answer» C. Mendel
14.

Which of the following is a Sanger’s reagent?$

A. 1-fluoro-2, 4-dinitrobenzene
B. 1-fluoro-2, 3-dinitrobenzene
C. 1-fluoro-2, 4-trinitrobenzene
D. 1-fluoro-2, 3-trinitrobenzene
Answer» B. 1-fluoro-2, 3-dinitrobenzene
15.

Amino acids sequence in DNA can be determined by the order of their

A. rRNA
B. tRNA
C. Nucleotides
D. mRNA
Answer» D. mRNA