Cleavage of DNA by RI endonuclease results in the formation of

one 3'-OH end and one 5'-phosphate end

two 3'-OH ends and two 5'-phosphate ends

one 3'-OH end and one 5'-phosphate end

two 3'-phosphate ends and two 3'-OH ends

one 3'-OH end and two 5'-phosphate end

Cleavage of DNA by EcoRI endonuclease results in the formation of

one 3'-OH end and one 5'-phosphate end

two 3'-OH ends and two 5'-phosphate ends

one 3'-OH end and one 5'-phosphate end

two 3'-phosphate ends and two 3'-OH ends

one 3'-OH end and two 5'-phosphate end

The type II restriction endonucleases, bind to DNA sites

using non-sequence-dependent backbone interactions

Reverse transcripase from HIV does not

has a 5' to 3' polymerase activity

has a 3'- 5' exonuclease activity

Explore topic-wise MCQs in Biochemistry.

This section includes 8 Mcqs, each offering curated multiple-choice questions to sharpen your Biochemistry knowledge and support exam preparation. Choose a topic below to get started.

1.	Cleavage of DNA by RI endonuclease results in the formation of
A.	two 3'-OH ends and two 5'-phosphate ends
B.	one 3'-OH end and one 5'-phosphate end
C.	two 3'-phosphate ends and two 3'-OH ends
D.	one 3'-OH end and two 5'-phosphate end
Answer» B. one 3'-OH end and one 5'-phosphate end

Discussion

2.	Cleavage of DNA by EcoRI endonuclease results in the formation of
A.	two 3'-OH ends and two 5'-phosphate ends
B.	one 3'-OH end and one 5'-phosphate end
C.	two 3'-phosphate ends and two 3'-OH ends
D.	one 3'-OH end and two 5'-phosphate end
Answer» B. one 3'-OH end and one 5'-phosphate end

Discussion

3.	Which of the following amino acids can interact with the DNA or RNA nucleotide bases via hydrogen bonding?
A.	Asn and Gln
B.	Cys and Met
C.	Lys and Leu
D.	Ile and Val
Answer» B. Cys and Met

Discussion

4.	The type II restriction endonucleases, bind to DNA sites
A.	using H-bonds for specificity
B.	that are usually palindromic
C.	using non-sequence-dependent backbone interactions
D.	all of the above
Answer» E.

Discussion

5.	The enzyme that joins DNA cuts is called
A.	joinase
B.	ligase
C.	DNA phosphorylase
D.	reverse transcriptase
Answer» C. DNA phosphorylase

Discussion

6.	Reverse transcripase from HIV does not
A.	uses RNA as a template
B.	has a 5' to 3' polymerase activity
C.	has a 3'- 5' exonuclease activity
D.	requires a primer
Answer» D. requires a primer

Discussion

7.	Different DNA polymerases play distinct roles in DNA replication and repair in both prokaryotic and eukaryotic cells. All known DNA polymerases synthesize DNA only in the __________ by the addition of dNTPs to a performed primer strand of DNA.
A.	positive direction
B.	3' to 5' direction
C.	5' to 3' direction
D.	negative direction
Answer» D. negative direction

Discussion

8.	Which of the following amino acids interact with the DNA or RNA backbone (i.e. phosphate)?
A.	Ile and Val
B.	Lys and Arg
C.	Leu and Ala
D.	Cys and Met
Answer» C. Leu and Ala

Discussion

Explore topic-wise MCQs in Biochemistry.

Cleavage of DNA by RI endonuclease results in the formation of

Cleavage of DNA by EcoRI endonuclease results in the formation of

Which of the following amino acids can interact with the DNA or RNA nucleotide bases via hydrogen bonding?

The type II restriction endonucleases, bind to DNA sites

The enzyme that joins DNA cuts is called

Reverse transcripase from HIV does not

Different DNA polymerases play distinct roles in DNA replication and repair in both prokaryotic and eukaryotic cells. All known DNA polymerases synthesize DNA only in the __________ by the addition of dNTPs to a performed primer strand of DNA.

Which of the following amino acids interact with the DNA or RNA backbone (i.e. phosphate)?