Explore topic-wise MCQs in Biochemistry.

This section includes 199 Mcqs, each offering curated multiple-choice questions to sharpen your Biochemistry knowledge and support exam preparation. Choose a topic below to get started.

51.

The deficiency of which of the following enzyme leads to galactosemia?

A. Galactokinase
B. Pyruvate kinase
C. PFK-1
D. PFK-2
Answer» B. Pyruvate kinase
52.

Which out of the following is not a flavoprotein?

A. Succinate dehydrogenase
B. Cytochrome c
C. Xanthine oxidase
D. NADH dehydrogenase-Qreductase
Answer» C. Xanthine oxidase
53.

What is the main source of natural fluoride?

A. Mushrooms
B. Potatoes
C. Meat
D. Water
Answer» E.
54.

Which of the following mineral is involved in protein metabolism and oxidation reactions?

A. Molybdenum
B. Manganese
C. Magnesium
D. calcium
Answer» B. Manganese
55.

One of the following statements is correct about pentose phosphate pathway

A. It generates 6 moles of CO₂ for each mole of glucose consumed
B. It generates 38 mol of ATP per mole of glucose consumed
C. It is a reductive pathway that consumes NADH
D. Present in plants but not in animals
Answer» B. It generates 38 mol of ATP per mole of glucose consumed
56.

Which of the following statements about the control of glycogen synthesis from blood glucose in myocytes is incorrect?

A. Insulin affects three of five steps in this pathway
B. The effects on transport and hexokinase activity increase the flux towards glycogen
C. The change in glycogen synthase activity increases the flux towards glycogen
D. Hexokinase enzyme is involved
Answer» D. Hexokinase enzyme is involved
57.

Iodine is a part of thyroid hormone and is essential for the prevention of

A. goiter
B. osteoporosis
C. muscle weakness
D. diarrhea
Answer» B. osteoporosis
58.

Which of the following statements is true about the control of muscle glycogen phosphorylase?

A. It is activated by phosphorylation by an active phosphorylase kinase
B. It is allosterically activated by ATP
C. It is allosterically activated by cAMP
D. Normally it exists in active form
Answer» B. It is allosterically activated by ATP
59.

Which of the following enzyme’s gene expression is slowed by insulin?

A. Hexokinase II
B. Hexokinase IV
C. PEP carboxykinase
D. Pyruvate kinase
Answer» D. Pyruvate kinase
60.

Selenium deficiency leads to

A. liver necrosis
B. diarrhea
C. multiple sclerosis
D. Crohn's disease
Answer» B. diarrhea
61.

∆G° is defined as the

A. Residual energy present in the reactants at equilibrium
B. Residual energy present in the products at equilibrium
C. Difference in the residual energy of reactants and products at equilibrium
D. Energy required in converting one mole of reactants to one mole of products
Answer» E.
62.

Which of the following is required as a component of blood hemoglobin?

A. Iron
B. Magnesium
C. Calcium
D. Copper
Answer» B. Magnesium
63.

Which is an example for chemical to osmotic energy conversion that occurs in living organisms?

A. ATP-driven muscle contraction
B. ATP-dependent photon emission in fireflies
C. light-induced electron flow in chloroplasts
D. ATP-driven active transport across a membrane
Answer» E.
64.

Ethanol is oxidized to acetaldehyde in the liver cytoplasm by

A. Alcohol dehydrogenase
B. Alcohol carboxylase
C. Pyruvate carboxylase
D. Pyruvate kinase
Answer» B. Alcohol carboxylase
65.

The mineral which aids in the utilization of iron and in hemoglobin synthesis is

A. calcium
B. phosphorus
C. cobalt
D. copper
Answer» E.
66.

The product formed in the isomerization of mannose 6-phosphate

A. Fructose 6-phosphate
B. Glucose 6-phosphate
C. 2-phosphoglycerate
D. 3-phosphoglycerate
Answer» B. Glucose 6-phosphate
67.

Which one out of the following enzymes acts in pentose phosphate pathway?

A. Aldolase
B. Glycogen phosphorylase
C. Pyruvate kinase
D. 6-phosphogluconate dehydrogenase
Answer» E.
68.

Which of the following mineral is involved in the enzyme -controlled energy yielding reactions of metabolism?

A. Calcium
B. Phosphorus
C. Iron
D. Copper
Answer» C. Iron
69.

Glucagon and epinephrine stimulate glycogen breakdown to glucose 6-phosphate

A. Directly by binding to glycogen phosphorylase
B. Indirectly by first stimulating adenylate cyclase to make cAMP
C. Only in the liver
D. Only in muscle cells
Answer» C. Only in the liver
70.

Which of the following is involved in pentose phosphate pathway?

A. Glucose 6-phosphate dehydrogenase
B. Acyl co-A glycerol transferases
C. PFK-2/FBPase-2
D. Pyruvate dehydrogenase
Answer» B. Acyl co-A glycerol transferases
71.

When one molecule of glucose is oxidized to two molecules of lactate during anaerobic glycolysis, which of the following statements is false?

A. Glyceraldehyde 3-P dehydrogenase reaction produces 2 ATP molecules
B. Lactate dehydrogenase reaction produces no ATP
C. Pyruvate kinase reaction produces 2 ATP molecules
D. Phosphofructokinase-1 reaction uses 1 ATP molecule
Answer» B. Lactate dehydrogenase reaction produces no ATP
72.

Examples of goitrogenic foods are __________ and __________ .

A. turnips and corn
B. rutabagas and okra
C. raw cabbage and turnips
D. cassava and lettuce
Answer» D. cassava and lettuce
73.

Hydrolysis of lactose yields

A. D-galactose and D-glucose
B. D-glucose and D-glucose
C. D-galactose and D-fructose
D. D-fructose and D-glucose
Answer» B. D-glucose and D-glucose
74.

Glucose 6-phosphatase is present only in

A. Liver
B. Muscle
C. Epithelial tissues
D. Kidneys
Answer» B. Muscle
75.

Which of the following is true about the enzyme producing NADH from a triose phosphate in the glycolytic pathway?

A. It produces 1, 3-biphosphoglycerate and NADH
B. It catalyzes irreversible reaction
C. It uses NAD⁺ and dihydroxyacetone phosphate as substrates
D. It uses FADH₂ and glyceraldehyde 3-phosphate as substrates
Answer» B. It catalyzes irreversible reaction
76.

When blood sugar levels fall, glycolysis is halted in liver to allow _________

A. Homeostasis
B. Anaerobic respiration
C. Aerobic respiration
D. Gluconeogenesis
Answer» E.
77.

Which of the following statements is correct about reductive pentose phosphate pathway?

A. It is not reversible
B. Transketolase transfers 3 carbon units
C. Transaldose transfers 2 carbon units
D. Pentoses can provide glycolytic intermediates
Answer» E.
78.

Which of the following type of metabolite is used for generating glucose under severe starvation conditions?

A. Amino acids
B. Fats
C. Glycogen
D. Starch
Answer» B. Fats
79.

Anabolism and catabolism are chemically linked in the form of

A. ADP
B. ATP
C. Phosphodiester linkage
D. ASP
Answer» C. Phosphodiester linkage
80.

Which of the following enzymes is involved in the positive regulation of glycolysis/gluconeogenesis?

A. Hexokinase II
B. Hexokinase IV
C. PFK-2/FBPase-2
D. Pyruvate kinase
Answer» D. Pyruvate kinase
81.

A kinase is an enzyme that

A. removes phosphate groups of substrates
B. uses ATP to add a phosphate group to the substrate
C. uses NADH to change the oxidation state of the substrate
D. removes water from a double bond
Answer» C. uses NADH to change the oxidation state of the substrate
82.

During catabolism, only about 40% of the energy available from oxidizing glucose is used to synthesize ATP. Remaining 60%

A. is lost as heat
B. is used to reduce NADP
C. remains in the products of metabolism
D. is stored as fat.
Answer» B. is used to reduce NADP
83.

The released energy obtained by oxidation of glucose is stored as

A. a concentration gradient across a membrane
B. ADP
C. ATP
D. NAD+
Answer» D. NAD+
84.

Which trace mineral is a component of the enzyme that activates vitamin A in the eye?

A. Zinc
B. Iron
C. Iodine
D. Chromium
Answer» B. Iron
85.

Which of the following vitamin is essential for the absorption of calcium from the intestinal tract?

A. Vitamin D
B. Vitamin E
C. Vitamin B12
D. Vitamin B6
Answer» B. Vitamin E
86.

Which of the following enzyme catalyzes the first step of glycolysis?

A. Hexokinase
B. Pyruvate kinase
C. Glucokinase
D. Phosphofructokinase-1
Answer» B. Pyruvate kinase
87.

The rate of breakdown of metabolites is termed as

A. Metabolic state
B. Metabolism
C. Steady state
D. Homeostasis
Answer» D. Homeostasis
88.

The substrate used in the last step of glycolysis is

A. Glyceraldehyde 3-phosphate
B. Pyruvate
C. Phosphoenolpyruvate
D. 1, 3-bisphosphoglycerate
Answer» D. 1, 3-bisphosphoglycerate
89.

Glycolytic pathway regulation involves

A. allosteric stimulation by ADP
B. allosteric inhibition by ATP
C. feedback, or product, inhibition by ATP
D. all of the above
Answer» E.
90.

Which of the following enzyme catalyses the direct transfer and incorporation of O₂ into a substrate molecule is

A. Reductase
B. Oxidase
C. Oxygenase
D. Peroxidase
Answer» D. Peroxidase
91.

What does first law of thermodynamics state?

A. Energy can neither be destroyed nor created
B. Energy cannot be 100 percent efficiently transformed from one type to another
C. All living organisms are composed of cells
D. Input of heat energy increases the rate of movement of atoms and molecules
Answer» B. Energy cannot be 100 percent efficiently transformed from one type to another
92.

Which One of the following statements about the control of enzyme activity by phosphorylation is correct

A. Phosphorylation of an enzyme results in conformational change
B. Phosphorylation of an enzyme occurs only at specific tyrosine residues
C. Phosphorylation of an enzyme is carried out by phosphoprotein phosphatases
D. Enzyme control by phosphorylation is irreversible
Answer» B. Phosphorylation of an enzyme occurs only at specific tyrosine residues
93.

Which of the following ion is required for the development of sound teeth with resistance to tooth decay?

A. Fluoride ion
B. Sodium ion
C. Chloride ion
D. Magnesium ion
Answer» B. Sodium ion
94.

Which of the following is necessary for the clotting of blood, functions of certain enzymes and control of fluids through cell membranes?

A. Calcium
B. Magnesium
C. Copper
D. Cobalt
Answer» B. Magnesium
95.

The enzyme which catalyzes the conversion of pyruvate to oxaloacetate

A. Pyruvate carboxylase
B. Pyruvate dehydrogenase
C. Pyruvate kinase
D. Phosphofructokinase-1
Answer» B. Pyruvate dehydrogenase
96.

How many steps are catalyzed by different enzymes in glycolysis and gluconeogenesis?

A. 2
B. 3
C. 4
D. 5
Answer» C. 4
97.

Which of the following statements is incorrect about oxidase?

A. They catalyze reactions involving hydrogen peroxide
B. They catalyze reaction using O₂ as a hydrogen acceptor
C. They catalyze reactions using niacin as coenzyme
D. They catalyze reactions of direct incorporation of O₂ into the substrate
Answer» C. They catalyze reactions using niacin as coenzyme
98.

Hydrolysis of sucrose yields

A. D-galactose and D-glucose
B. D-glucose and D-glucose
C. D-galactose and D-fructose
D. D-fructose and D-glucose
Answer» E.
99.

Conversion of xylulose 5-phosphate to ribulose 5-phosphate is catalyzed by

A. Phosphopentose epimerase
B. Transaldolase
C. Transketolase
D. Phosphopentose isomerase
Answer» B. Transaldolase
100.

Which of the following conversions is not catalyzed by hexokinase?

A. D-Glucose to glucose 6-phosphate
B. D-Fructose to fructose 6-phosphate
C. D-Mannose to mannose 6-phosphate
D. D-Fructose to fructose 1-phosphate
Answer» E.