Explore topic-wise MCQs in Microbiology.

This section includes 7 Mcqs, each offering curated multiple-choice questions to sharpen your Microbiology knowledge and support exam preparation. Choose a topic below to get started.

1.

Polymerase chain reaction basically consists of
A. two steps
B. three steps
C. four steps
D. five steps
Answer» C. four steps
Discussion
2.

The efficiency of amplification in PCR in later cycles is reduced due to
A. reduction in substrate concentration
B. insufficient enzyme and time to synthesize mass quantity of DNA
C. build up of PCR product which competes with primers for hybrid formation
D. all of these
Answer» E.
Discussion
3.

RNA populations can also be used as PCR templates after reverse transcription into
A. c DNA
B. t RNA
C. m DNA
D. r RNA
Answer» B. t RNA
Discussion
4.

The cloning step in PCR/sequencing analysis of microbial communities is necessary for
A. the amplification process
B. preventing contamination by outside DNA
C. separating the different rRNA gene sequences in the mixture
D. none of these
Answer» D. none of these
Discussion
5.

The template for PCR is
A. RNA
B. single stranded DNA
C. double stranded DNA
D. none of these
Answer» D. none of these
Discussion
6.

The PCR/sequencing approach to rRNA is most likely to give information about
A. the species to which the bacteria are most closely related
B. morphology of the bacterium
C. the type of energy metabolism the bacterium has
D. whether the bacterium is motile
Answer» B. morphology of the bacterium
Discussion
7.

Which of the following infection(s) can be diagnosed by the use of polymerase chain reaction?
A. HIV-1 and HIV-2 viruses
B. Hepatitis B virus
C. <i>Mycobacterium tuberculosis</i>
D. All of these
Answer» E.
Discussion