acquiring DNA from the bacterial cell environment

A PCR cycle consists of

three steps, denaturation, initiation and elongation

three steps, denaturation, primer annealing and elongation

three steps, denaturation, initiation and elongation

three steps, primer annealing, elongation and termination

three steps, initiation, elongation and termination

cells in the blood that eat bacteria

medical diagnosis and forensic medicine

Southern blotting involves

electrophoresis of DNA molecules and then blotting the separated RNA bands followed by incubation

electrophoresis of DNA molecules and then blotting the separated DNA bands followed by incubation

electrophoresis of DNA molecules and then blotting the separated RNA bands followed by incubation

electrophoresis of DNA molecules followed by incubation

electrophoresis of RNA molecules and then blotting the separated RNA bands followed by incubation

To clone into a plasmid vector, both the plasmid and the foreign DNA are cut

with different restriction enzyme and mixed together

with the same restriction enzyme and mixed together

with different restriction enzyme and mixed together

with the combination of enzymes and then seperated

with the combination of enzymes and mixed together

Bactriophages absorb to a bacterial surface and inject the phage DNA through the

cell wall into endoplasmic reticulum

Additional mono saccharides are added in Golgi to produce

either fructose type monosaccharide or a complex type

either high mannose type oligosaccharide or a complex type

either fructose type monosaccharide or a complex type

either high sucrose type polysaccharide or a complex type

Specialized transduction occurs when

the bacteriophage incorporates randomly in the bacterial chromosome

the bacteriophage never incorporates into the bacterial chromosome

the bacteriophage always incorporates at the same position in the bacterial chromosome

For gene transfer to be effective, transforming DNA must be

incorporated into a viral genome

incorporated into the bacterial chromosome

incorporated into a viral genome

free in the bacterial cytoplasm

PCR amplifies millions of copies of

the entire genome (all the DNA in a sample)

allows comparison between DNA molecules but requires to determine nucleotide sequence

allows comparison between DNA molecules without the need to determine nucleotide sequence

allows comparison between DNA molecules but requires to determine nucleotide sequence

does not allow comparison between DNA molecules

widely different than southern blotting

another name for southern blotting

26 + Mcqs in Polymerase Chain Reaction in Biochemistry Page 1 McqOptions

1.	Transformation means
A.	formation of a pilus
B.	acquiring DNA from the bacterial cell environment
C.	plasmid containing a F factor
D.	F+ and F- strains of bacteria
Answer» C. plasmid containing a F factor

Discussion

2.	A PCR cycle consists of
A.	three steps, denaturation, primer annealing and elongation
B.	three steps, denaturation, initiation and elongation
C.	three steps, primer annealing, elongation and termination
D.	three steps, initiation, elongation and termination
Answer» B. three steps, denaturation, initiation and elongation

Discussion

3.	Bacteriophages are
A.	cells in the blood that eat bacteria
B.	a class of bacteria
C.	bacterial viruses
D.	none of the above
Answer» D. none of the above

Discussion

4.	PCR can be used in
A.	cloning
B.	sequencing
C.	medical diagnosis and forensic medicine
D.	all of the above
Answer» E.

Discussion

5.	PCR (Polymerase Chain Reaction) can make multiple copies of
A.	DNA
B.	RNA
C.	protein
D.	carbohydrate
Answer» B. RNA

Discussion

6.	PCR is an abbreviation for
A.	Protein Change Reaction
B.	Polymerase Chain Reaction
C.	Positive Charge Reaction:
D.	Preliminary Change Rate
Answer» C. Positive Charge Reaction:

Discussion

7.	Southern blotting involves
A.	electrophoresis of DNA molecules and then blotting the separated DNA bands followed by incubation
B.	electrophoresis of DNA molecules and then blotting the separated RNA bands followed by incubation
C.	electrophoresis of DNA molecules followed by incubation
D.	electrophoresis of RNA molecules and then blotting the separated RNA bands followed by incubation
Answer» B. electrophoresis of DNA molecules and then blotting the separated RNA bands followed by incubation

Discussion

8.	PCR is used
A.	to diagnose genetic diseases
B.	to solve crimes
C.	to study gene function
D.	all of these
Answer» E.

Discussion

9.	To clone into a plasmid vector, both the plasmid and the foreign DNA are cut
A.	with the same restriction enzyme and mixed together
B.	with different restriction enzyme and mixed together
C.	with the combination of enzymes and then seperated
D.	with the combination of enzymes and mixed together
Answer» B. with different restriction enzyme and mixed together

Discussion

10.	PCR can be used to amplify a specific fragment of DNA from which of the following?
A.	A drop of blood
B.	A hair follicle
C.	A fragment of skin
D.	All of these
Answer» E.

Discussion

11.	Bactriophages absorb to a bacterial surface and inject the phage DNA through the
A.	cell wall into cytosol
B.	cell wall into plasmamembrane
C.	both (a) and (b)
D.	cell wall into endoplasmic reticulum
Answer» B. cell wall into plasmamembrane

Discussion

12.	Additional mono saccharides are added in Golgi to produce
A.	either high mannose type oligosaccharide or a complex type
B.	either fructose type monosaccharide or a complex type
C.	either high sucrose type polysaccharide or a complex type
D.	none of the above
Answer» B. either fructose type monosaccharide or a complex type

Discussion

13.	The PCR, polymerase chain reaction is becoming the method of choice for
A.	Alteration of gene
B.	screening gene
C.	sterilization of gene
D.	all of these
Answer» C. sterilization of gene

Discussion

14.	Specialized transduction occurs when
A.	the bacteriophage incorporates randomly in the bacterial chromosome
B.	the bacteriophage never incorporates into the bacterial chromosome
C.	the bacteriophage always incorporates at the same position in the bacterial chromosome
D.	none of the above
Answer» D. none of the above

Discussion

15.	DNA fragments in a restriction digest can be separated by electrophoresis in
A.	poly acrylamide
B.	agarose gel
C.	both (a) and (b)
D.	none of these
Answer» D. none of these

Discussion

16.	Double stranded DNA denaturation with specified limit of temperature is
A.	reversible reaction
B.	irreversible reaction
C.	either (a) or (b)
D.	none of these
Answer» B. irreversible reaction

Discussion

17.	Genomic libraries are made from
A.	genomic DNA of an organism
B.	genomic RNA of an organism
C.	genomic cDNA of an organism
D.	genomic mRNA of an organism
Answer» B. genomic RNA of an organism

Discussion

18.	The viruses that can achieve neoplastic transformation are called
A.	DNA tumor viruses
B.	RNA tumor viruses
C.	retroviruses HIV
D.	none of these
Answer» B. RNA tumor viruses

Discussion

19.	From a single molecule of DNA, PCR can make
A.	one additional copy
B.	hundreds of copies
C.	thousands of copies
D.	millions of copies
Answer» E.

Discussion

20.	For gene transfer to be effective, transforming DNA must be
A.	incorporated into the bacterial chromosome
B.	incorporated into a viral genome
C.	free in the bacterial cytoplasm
D.	none of the above
Answer» B. incorporated into a viral genome

Discussion

21.	PCR amplifies millions of copies of
A.	a short fragment of DNA
B.	the entire genome (all the DNA in a sample)
C.	both (a) and (b)
D.	short chain RNA
Answer» B. the entire genome (all the DNA in a sample)

Discussion

22.	Restriction maps
A.	allows comparison between DNA molecules without the need to determine nucleotide sequence
B.	allows comparison between DNA molecules but requires to determine nucleotide sequence
C.	does not allow comparison between DNA molecules
D.	none of the above
Answer» B. allows comparison between DNA molecules but requires to determine nucleotide sequence

Discussion

23.	How many ds DNA molecule can be produced, which comprise precisely the target region in double strand form during 4th cycle of PCR?
A.	Two ds DNA molecule
B.	Three ds DNA molecule
C.	Eight ds DNA molecule
D.	Four ds DNA molecule
Answer» E.

Discussion

24.	In a PCR reaction after four cycles, each molecule of a duplex DNA will give rise to
A.	16 single strands of DNA
B.	16 double stranded DNA
C.	18 single strands of DNA
D.	18 double stranded DNA
Answer» B. 16 double stranded DNA

Discussion

25.	Northern blotting is
A.	widely different than southern blotting
B.	another name for southern blotting
C.	analogous to southern blotting
D.	none of the above
Answer» D. none of the above

Discussion

26.	What technique can be used to determine the murderer who left blood with the victim?
A.	DNA sequencing
B.	PCR amplification
C.	Western blot
D.	RFLP mapping
Answer» C. Western blot

Discussion

Explore topic-wise MCQs in Biochemistry.

Transformation means

A PCR cycle consists of

Bacteriophages are

PCR can be used in

PCR (Polymerase Chain Reaction) can make multiple copies of

PCR is an abbreviation for

Southern blotting involves

PCR is used

To clone into a plasmid vector, both the plasmid and the foreign DNA are cut

PCR can be used to amplify a specific fragment of DNA from which of the following?

Bactriophages absorb to a bacterial surface and inject the phage DNA through the

Additional mono saccharides are added in Golgi to produce

The PCR, polymerase chain reaction is becoming the method of choice for

Specialized transduction occurs when

DNA fragments in a restriction digest can be separated by electrophoresis in

Double stranded DNA denaturation with specified limit of temperature is

Genomic libraries are made from

The viruses that can achieve neoplastic transformation are called

From a single molecule of DNA, PCR can make

For gene transfer to be effective, transforming DNA must be

PCR amplifies millions of copies of

Restriction maps

How many ds DNA molecule can be produced, which comprise precisely the target region in double strand form during 4th cycle of PCR?

In a PCR reaction after four cycles, each molecule of a duplex DNA will give rise to

Northern blotting is

What technique can be used to determine the murderer who left blood with the victim?