

MCQOPTIONS
This section includes 50 Mcqs, each offering curated multiple-choice questions to sharpen your Microbiology knowledge and support exam preparation. Choose a topic below to get started.
1. |
If a 1:600 dilution of a test compound kills a standard population of in 10 minutes but not 5 minutes while a 1:60 dilution of phenol kills the population in the same time, what is the phenol coefficient of the test compound? |
A. | 1 |
B. | 5 |
C. | 10 |
D. | 50 |
Answer» D. 50 | |
2. |
Which disinfectant was the most effective against "? |
A. | Phenol |
B. | Cetylpyridinium chloride |
C. | Hexachlorophene |
D. | Merthiolate |
Answer» E. | |
3. |
Which disinfectant would be most effective against found in a blood spill? |
A. | Phenol |
B. | Cetylpyridinium chloride |
C. | Hexachlorophene |
D. | None of these |
Answer» E. | |
4. |
The time required to kill 90% of the microorganisms in a sample at a specific temperature is the |
A. | decimal reduction time |
B. | thermal death point |
C. | F value |
D. | D value |
Answer» E. | |
5. |
Which of the following articles can be sterilized in an autoclave? |
A. | Gloves |
B. | Culture media |
C. | Dressing material |
D. | All of these |
Answer» E. | |
6. |
Which of the following disinfectants act by disrupting microbial membranes? |
A. | Cationic detergents |
B. | Halogens |
C. | Heavy metals |
D. | Aldehydes |
Answer» B. Halogens | |
7. |
Which of the following is best to sterilize heat labile solutions? |
A. | Dry heat |
B. | Autoclave |
C. | Membrane filtration |
D. | Pasteurization |
Answer» D. Pasteurization | |
8. |
Which of the following is best used for long term storage of microbial samples when carried out properly? |
A. | Storage in a freezer at -10 C |
B. | Storage in a freezer at ultra low temperatures (-70 C) |
C. | Storage in a refrigerator on an agar slant |
D. | Storage on a petri plate at room temperature |
Answer» C. Storage in a refrigerator on an agar slant | |
9. |
Which of the following compounds are commonly used as general antiseptics to treat cuts and scratches? |
A. | Aldehydes |
B. | Ethylene oxide |
C. | Halogens |
D. | None of these |
Answer» D. None of these | |
10. |
Which of the following is a limitation of the autoclave? |
A. | It takes too long to sterilize |
B. | It lacks the ability to inactivate viruses |
C. | It lacks the ability to kill endospores |
D. | It will destroy heat labile materials |
Answer» E. | |
11. |
Which of the following has most heat resistant spores? |
A. | <i>Clostridium histolyticum</i> |
B. | <i>Clostridium perfringens </i> |
C. | <i>Bacillus stearothermophilus</i> |
D. | <i> Bacillus cereus</i> |
Answer» D. <i> Bacillus cereus</i> | |
12. |
The lowest temperature that kills all microorganisms in a liquid suspension in 10 minutes is known as the |
A. | decimal reduction time |
B. | thermal death point |
C. | thermal death temperature |
D. | thermal death time |
Answer» C. thermal death temperature | |
13. |
Which of the following tests is designed to simulate the natural conditions under which the disinfectants are used in hospitals? |
A. | Phenol coefficient test |
B. | Minimum inhibitory concentration test |
C. | Capacity test |
D. | None of these |
Answer» D. None of these | |
14. |
Which of the following was the first widely used antiseptic and disinfectant? |
A. | Chlorine |
B. | Phenol |
C. | Iodine |
D. | Alcohol |
Answer» C. Iodine | |
15. |
Steam exposure of a material at 100 C for 20 minutes for three consecutive days is known as |
A. | inspissation |
B. | tyndallization |
C. | autoclaving |
D. | none of these |
Answer» C. autoclaving | |
16. |
The organisms retained in the fluids filtered by Seitz filter is |
A. | <i>Proteus</i> |
B. | <i>Staphylococcus</i> |
C. | <i>Clostridium</i> |
D. | None of these |
Answer» E. | |
17. |
For sterilization of which material gamma rays can be used? |
A. | Catheters |
B. | Plastic syringes |
C. | Canulas |
D. | None of these |
Answer» E. | |
18. |
The time in minutes at a specific temperature needed to kill a population of cells is the |
A. | decimal reduction time |
B. | thermal death point |
C. | thermal death temperature |
D. | F value |
Answer» E. | |
19. |
Which of the following is sterilized with the help of glutaraldehyde? |
A. | Thermometers |
B. | Bronchoscopes |
C. | Cystoscopes |
D. | All of these |
Answer» E. | |
20. |
Microbes can be removed from a liquid solution by the process of |
A. | filtration |
B. | freeze-drying |
C. | osmosis |
D. | desiccation |
Answer» B. freeze-drying | |
21. |
Which of the following process can be efficiently carried out by incineration? |
A. | Sterilization of scalpel blades and needles |
B. | Sterilization of all glass syringes |
C. | Sterilization of points of forceps |
D. | Destruction of contaminated materials |
Answer» E. | |
22. |
The process of making an object free from living organisms including bacterial and fungal spores and viruses is known as |
A. | pasteurization |
B. | antisepsis |
C. | disinfection |
D. | sterilization |
Answer» E. | |
23. |
Media containing spores and thermolabile constituents are sterilized by |
A. | pasteurization |
B. | UV radiation |
C. | dry heat |
D. | tyndalization |
Answer» C. dry heat | |
24. |
A(n)____is used to prevent infection by killing or inhibiting pathogen growth on animal tissues. |
A. | bacteriostatic agent |
B. | sanitizer |
C. | disinfectant |
D. | antiseptic |
Answer» E. | |
25. |
One drawback to the use of ultraviolet light as a sterilizing agent is its |
A. | failure to kill bacteria |
B. | failure to bacterial spores |
C. | failure to kill microbes in a closed environment |
D. | failure to kill microbes located in the center of an object |
Answer» E. | |
26. |
Ethylene oxide is used to destroy or kill which of the following microbes? |
A. | Bacteria |
B. | Spores |
C. | Fungi |
D. | All of these |
Answer» E. | |
27. |
Bactericidal concentration of phenol is |
A. | 0.25% |
B. | 0.5% |
C. | 0.75% |
D. | 1% |
Answer» E. | |
28. |
Which of the following skin disinfectant(s) is/are most frequently used? |
A. | Isopropyl alcohol |
B. | Ethyl alcohol |
C. | Both (a) and (b) |
D. | none of these |
Answer» D. none of these | |
29. |
Preservation of foods by using salts and sugars works by |
A. | raising pH |
B. | lowering osmotic pressure |
C. | creating a hypertonic environment |
D. | creating a hypotonic environment |
Answer» D. creating a hypotonic environment | |
30. |
Which of the following items could be sterilized by dry heat sterilization? |
A. | Intravenous (IV) solution |
B. | Plastic IV bags |
C. | Glass pipettes |
D. | rubber gloves |
Answer» D. rubber gloves | |
31. |
Which of the following does not kill endospores? |
A. | Autoclave |
B. | Incineration |
C. | Hot air sterilization |
D. | Pasteurization |
Answer» E. | |
32. |
Which of the following describes the effect of ionizing radiation on microbes? |
A. | Changes the 3-dimensional structure of proteins to a 2-dimen-sional form |
B. | Substantially decreases enzymatic activity, slowing microbial reproduction |
C. | Induces electrons and protons to jump out of the molecules of the microbe |
D. | None of the above |
Answer» D. None of the above | |
33. |
Which is the most important surface active disinfectants? |
A. | Amphoteric compounds |
B. | Cationic compounds |
C. | Non-ionic compounds |
D. | Anionic compounds |
Answer» C. Non-ionic compounds | |
34. |
Milk is pasteurized in batch method by keeping it at |
A. | 63 C for 30 minutes |
B. | 72 C for 60 seconds |
C. | 73 C for 30 minutes |
D. | 72 C for 6 minutes |
Answer» B. 72 C for 60 seconds | |
35. |
Which disinfectant was the most effective against Salmonella"? |
A. | Phenol |
B. | Cetylpyridinium chloride |
C. | Hexachlorophene |
D. | Merthiolate |
Answer» E. | |
36. |
If a canning procedure is not properly followed, which type of microbe is most likely to grow in the canned food? |
A. | Obligate Aerobe |
B. | Acidophile |
C. | Mesophile |
D. | Obligate Anaerobe |
Answer» E. | |
37. |
Which of the following is not a disinfectant containing a heavy metal? |
A. | Silver nitrate |
B. | Mercurochrome |
C. | Copper sulfate |
D. | Chlorine |
Answer» E. | |
38. |
Which disinfectant would be most effective against Staphylococcus found in a blood spill? |
A. | Phenol |
B. | Cetylpyridinium chloride |
C. | Hexachlorophene |
D. | None of these |
Answer» E. | |
39. |
Complete destruction of the microorganisms is commonly known as |
A. | disinfection |
B. | sterilization |
C. | antisepsis |
D. | none of these |
Answer» D. none of these | |
40. |
Which of the following material is sterilized by use of red heat? |
A. | Soiled dressings |
B. | Inoculating wires and loops |
C. | Glass slides |
D. | All-glass syringes |
Answer» C. Glass slides | |
41. |
Which of the following methods will not sterilize? |
A. | Aqueous glutaraldehyde for 10 hours |
B. | Dry heat at 171 C for 1 to 2 hours |
C. | 121 C at 15 lb/in pressure for 15 to 20 minutes |
D. | 100 C boiling water for 30 minutes |
Answer» E. | |
42. |
Which of the following is/are vapour-phase disinfectant/s? |
A. | Formaldehyde |
B. | Ethylene oxide |
C. | Both (a) and (b) |
D. | None of these |
Answer» D. None of these | |
43. |
Sweet and salty foods frequently don't require refrigeration to prevent spoilage because they have |
A. | insufficient nutrients |
B. | low pH |
C. | high concentration of solutes |
D. | toxic alkaline chemicals |
Answer» D. toxic alkaline chemicals | |
44. |
Iodophores are mixture of |
A. | iodine and aldehydes |
B. | iodine and surface active agents |
C. | iodine and alcohols |
D. | iodine and phenols |
Answer» C. iodine and alcohols | |
45. |
If a 1:600 dilution of a test compound kills a standard population of Staphylococcus aureus in 10 minutes but not 5 minutes while a 1:60 dilution of phenol kills the population in the same time, what is the phenol coefficient of the test compound? |
A. | 1 |
B. | 5 |
C. | 10 |
D. | 50 |
Answer» D. 50 | |
46. |
Which of the following substances can sterilize? |
A. | Alcohol |
B. | Cetylpyridinium chloride |
C. | Ethylene oxide |
D. | Chlorine |
Answer» D. Chlorine | |
47. |
Which of the following is most effective for sterilizing mattresses and plastic petri plates? |
A. | Chlorine |
B. | Ethylene oxide |
C. | Glutaraldehyde |
D. | Ultraviolet radiation |
Answer» C. Glutaraldehyde | |
48. |
All of the following are sporicidal except |
A. | glutaraldehyde |
B. | ethylene oxide |
C. | formaldehyde |
D. | alcohol |
Answer» E. | |
49. |
Which of the following is bactericidal? |
A. | Membrane filtration |
B. | Ionizing radiation |
C. | Freeze-drying |
D. | Deep freezing |
Answer» C. Freeze-drying | |
50. |
Which of the following material is sterilized by heating at 160 C in a hot air oven for one hour? |
A. | Swab sticks |
B. | All-glass syringes |
C. | Oils and jellies |
D. | All of these |
Answer» E. | |