Explore topic-wise MCQs in Biochemistry.

This section includes 195 Mcqs, each offering curated multiple-choice questions to sharpen your Biochemistry knowledge and support exam preparation. Choose a topic below to get started.

1.

In what form does the product of glycolysis enter the TCA cycle?

A. AcetylCoA
B. Pyruvate
C. NADH
D. Glucose
Answer» B. Pyruvate
2.

Which of the following takes place in substrate level phosphorylation?

A. Oxidation of one molecule of substrate is linked to synthesis of more than one ATP molecule
B. High energy intermediate compounds cannot be isolated
C. Only mitochondrial reactions participate in ATP formation
D. Substrate reacts to form a product containing a high energy bond
Answer» E.
3.

The pigment used in human vision is

A. Retinal
B. Vitamin A
C. Ferredoxin
D. Beta-carotene
Answer» B. Vitamin A
4.

The followings are the products of the light reactions of photosynthesis except

A. ATP
B. oxygen
C. NADPH
D. glucose
Answer» E.
5.

The regulation of oxidative phosphorylation depends on

A. Magnitude of ion motive force
B. Magnitude of electron motive force
C. Magnitude of proton motive force
D. None of the mentioned
Answer» D. None of the mentioned
6.

A water-soluble protein that carries one electron present in mitochondria is

A. PQʙ
B. Q
C. Cytochrome c
D. Plastocyanin
Answer» D. Plastocyanin
7.

Carbon dioxide is reduced in

A. noncyclic photophosphorylation
B. the Calvin cycle
C. the light reactions
D. both light and dark reactions
Answer» C. the light reactions
8.

Maximum energy per gram on oxidation is yielded from

A. Starch
B. Fat
C. Glycogen
D. Protein
Answer» C. Glycogen
9.

Which of the following serves as a reactant in photosynthesis and a product in cellular respiration?

A. O₂
B. CO₂
C. Sunlight
D. ATP
Answer» C. Sunlight
10.

Oxidative phosphorylation is to respiration as __________ is to photosynthesis

A. carbon fixation
B. electron transport chain
C. light capture by chlorophyll
D. reduction of NADPH
Answer» C. light capture by chlorophyll
11.

What process in cellular respiration is essentially the reverse of carbon fixation in photosynthesis?

A. Glycolysis
B. Citric acid cycle
C. Oxidative phosphorylation
D. Alcohol fermentation
Answer» C. Oxidative phosphorylation
12.

Which of the following describes the complete route by which electrons flow H₂O to NADP⁺?

A. Z scheme
B. A scheme
C. B scheme
D. X scheme
Answer» B. A scheme
13.

Oxidative stress (in humans) is not involved in the development of

A. Cancer
B. Parkinson’s disease
C. Heart failure
D. Kidney failure
Answer» E.
14.

What is the proportion of ATP produced by oxidative phosphorylation?

A. 60%
B. 70%
C. 80%
D. 90%
Answer» B. 70%
15.

Which one of the following is a product of both cyclic and noncyclic photophosphorylation?

A. NADPH
B. O₂
C. ATP
D. Carbohydrate
Answer» D. Carbohydrate
16.

The end products of noncyclic photophosphorylation are

A. O₂, ATP and NADPH
B. carbon dioxide, PGAL (phosphoglyceraldehyde), and H2
C. water, ADP and NADP+
D. carbon dioxide, ATP and water
Answer» B. carbon dioxide, PGAL (phosphoglyceraldehyde), and H2
17.

The effect of increased levels of hydrogen ions in the inter-membrane space of the mitochondria is

A. Increase ATP production
B. Decreased levels of oxidative phosphorylation
C. Increased levels of water in inter-membrane space
D. Decreased levels of chemiosmosis
Answer» B. Decreased levels of oxidative phosphorylation
18.

Which of the following accepts only one electron?

A. Cytochrome b
B. Coenzyme Q
C. FMN
D. FAD
Answer» B. Coenzyme Q
19.

NADP is a cofactor used in

A. Catabolic reactions
B. Anabolic reactions
C. Elimination reaction
D. Redox reactions
Answer» C. Elimination reaction
20.

NADP⁺ in its reduced form is

A. NAD
B. NADH
C. NADPH
D. None of the mentioned
Answer» D. None of the mentioned
21.

Which of the following is the prosthetic group of NADH dehydrogenase?

A. NADH
B. FAD
C. NADPH
D. FMN
Answer» E.
22.

If rotenone is added to the mitochondrial electron transport chain

A. Succinate oxidation remains normal
B. P: O ratio of NADH is reduced from 3:1 to 2:1
C. Oxidative phosphorylation is uncoupled at site I
D. Rate of NADH oxidation is diminished to two-thirds of its initial value
Answer» B. P: O ratio of NADH is reduced from 3:1 to 2:1
23.

The transport of acyl co-A for oxidation using a shuttle involves formation of the intermediate

A. Acyl coenzyme A
B. 3 acetyl co-A
C. Acyl cartinine
D. None of the mentioned
Answer» D. None of the mentioned
24.

What do NAD+, NADP+, and FAD+ all have in common?

A. They are reduced
B. They have a full complement of electrons
C. They are oxidized
D. They are what is used during carbon fixation in photosynthesis
Answer» D. They are what is used during carbon fixation in photosynthesis
25.

The proposal of chemiosmotic hypothesis was by

A. Peter D. Mitchell
B. Charles Darwin
C. Mendele
D. Alfred Russell
Answer» B. Charles Darwin
26.

Complex 3 is also called _________

A. NADH dehydrogenase
B. Succinate dehydrogenase
C. Cytochrome bc1 complex
D. Cytochrome oxidase
Answer» D. Cytochrome oxidase
27.

The catabolism of sugars and fatty acids is similar because

A. both of these compounds are funnelled through the TC A/citric acid cycle
B. both of these compounds generate redox energy during catabolism
C. both of these compounds generate chemical energy during catabolism
D. all of the above
Answer» B. both of these compounds generate redox energy during catabolism
28.

Where do the protons come from that make up the proton gradient used in the light reactions of photosynthesis?

A. Glucose
B. ATP
C. H₂O
D. NADPH
Answer» D. NADPH
29.

A water-soluble protein that carries one electron present in chloroplast is

A. PQʙ
B. Q
C. Cytochrome c
D. Plastocyanin
Answer» E.
30.

A mobile, lipid soluble carrier of two electrons present in chloroplasts is

A. PQʙ
B. Q
C. Cytochrome c
D. Plastocyanin
Answer» B. Q
31.

One of the following is the source of electrons in photosynthesis

A. Carbohydrates
B. CO₂
C. Water
D. NADH
Answer» D. NADH
32.

Which of the following structures or processes are logically associated with chloroplasts?

A. Plant cells
B. Chlorophyll
C. Thylakoid membranes
D. All of these
Answer» E.
33.

Hydrogen is transferred from the light reaction to the dark reaction in photosynthesis by

A. DPN
B. NAD
C. ATP
D. NADP
Answer» B. NAD
34.

The size of plant mitochondrial DNA is

A. 16kb
B. 200-2500kb
C. 100- 2550kb
D. 100-2500kb
Answer» C. 100- 2550kb
35.

Plastoquinol formed in PSII is oxidized by

A. Cytochrome b₆f
B. Cytochrome c
C. Cytochrome f
D. None of the mentioned
Answer» B. Cytochrome c
36.

In algae, photosynthesis takes place in

A. choloroplasts
B. cell membrane
C. mitochondria
D. none of the above
Answer» B. cell membrane
37.

Assume the combined processes of photosynthesis and cellular respiration, the electrons that start as part of H2O at the beginning of the light reactions end up attaching to

A. O₂ to make new H₂O
B. NADPH to make new glucose
C. pyruvate to make ethanol
D. electron transport carriers to make O₂
Answer» B. NADPH to make new glucose
38.

Every cycle of β-oxidation produces

A. 1 FAD, 1 NAD⁺ and 2 CO₂ molecules
B. 1 FADH₂, 1 NADH and 1 acetyl co-A
C. 1 FADH₂, 1 NAD⁺ and 1 acetyl co-A
D. 1 FAD, 1 NADH and 2 CO₂ molecules
Answer» C. 1 FADH₂, 1 NAD⁺ and 1 acetyl co-A
39.

Which enzyme is involved in carbon-fixation reaction?

A. NADP reductase
B. Cytochrome reductase
C. Ribulose bisphosphate carboxylase
D. Glycerol kinase
Answer» D. Glycerol kinase
40.

Calvin cycle occurs in

A. Cytoplasm
B. Chloroplast
C. Mitochondria
D. Golgi body
Answer» B. Chloroplast
41.

Complex 2 is also called _________

A. NADH dehydrogenase
B. Succinate dehydrogenase
C. Cytochrome bc1 complex
D. Cytochrome oxidase
Answer» C. Cytochrome bc1 complex
42.

Energy that is released from glucose during respiration but not transferred to ATP bonds can be detected as

A. CO₂
B. AMP
C. ADP
D. heat
Answer» E.
43.

The size of human mitochondrial DNA is

A. 16kb
B. 200kb
C. 2500kb
D. 100kb
Answer» B. 200kb
44.

A soluble Cu-containing electron transfer protein is

A. Pheophytin
B. Cytochrome c
C. Plastocyanin
D. Ferredoxin
Answer» D. Ferredoxin
45.

During the light-dependent reactions of photosynthesis, which of the following does not occur?

A. Splitting of water
B. Carbon dioxide fixation
C. Release of oxygen
D. Absorption of light energy by photosystems
Answer» C. Release of oxygen
46.

Complex 1 is also called _________

A. NADH dehydrogenase
B. Succinate dehydrogenase
C. Cytochrome bc1 complex
D. Cytochrome oxidase
Answer» B. Succinate dehydrogenase
47.

Cholesterol can be synthesized de novo in

A. pancreas
B. intestine
C. liver
D. cell membrane
Answer» D. cell membrane
48.

Photosystem II is a

A. Pheophytin-quinone type
B. Ferredoxin type
C. Plastocyanin type
D. Plastocyanin-ferredoxin type
Answer» B. Ferredoxin type
49.

Which one of the following is true about C₄ plants?

A. They minimize rubisco oxygenase activity by fixing CO₂ into oxaloacetate
B. They mainly occur in areas of high temperature and light intensity
C. They minimize the rubisco oxygenase activity by fixing CO₂ into phosphoenolpyruvic acid
D. C₃ plants are more efficient in photosynthesis than C₄ plants
Answer» B. They mainly occur in areas of high temperature and light intensity
50.

When compared to retinal, chlorophyll can be described as a pigment that has a

A. Narrow absorption range but high efficiency
B. Narrow absorption range but low efficiency
C. Wide absorption range but high efficiency
D. Wide absorption range but low efficiency
Answer» B. Narrow absorption range but low efficiency